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Urinary levels of TGF β-1 and of cytokines in patients with prenatally detected nephrouropathies

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Abstract

This study aimed to identify noninvasive biomarkers of clinically significant nephrouropathies in patients with antenatal renal and/or urinary tract alterations. Spot-urine levels of interleukin-6 (IL-6), transforming growth factor-β1 (TGF-β1) and tumor necrosis factor-α (TNF-α) were measured in 100 patients with antenatal detected nephrouropathies. Patients were divided in idiopathic hydronephrosis (n = 47), urinary tract malformations (n = 35), and dysplastic kidneys (n = 18). Urinary concentrations of TGF-β1, IL-6, and TNF-α were compared between groups according to clinical and image findings. Receiver-operating characteristic (ROC) curves were analyzed for the overall diagnostic accuracy of TGF-β1, IL-6, and TNF-α levels in discriminating infants with nephrouropathies. No significant differences in urinary TGF- β1, IL-6, and TNF-α levels were found in the comparison between the groups. TGF-β1 levels tended to be higher in patients with renal hypodysplasia compared to idiopathic hydronephrosis (p = 0.07). Twenty-nine patients had reduced DMSA uptake. In these cases, absolute urinary concentration of TGF-β1 and levels standardized for creatinine were significantly higher than in patients with normal DMSA uptake, while IL6 and TNF-α did not differ between groups. Urinary cytokine measurements were not useful as a screening test for clinically significant nephrouropathies. Conversely, increased concentrations of TGF-β1 pointed out to renal damage as indicated by reduced DMSA uptake.

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Acknowledgements

This study was partially supported by CNPq (Brazilian National Research Council) and FAPEMIG. LRM, FFS, JMO and FFC were recipients of CNPq fellowships. Dr. E.A. Oliveira, Dr. A.C. Simões e Silva and Dr. M.M. Teixeira received a research grant from the Brazilian Research Council (CNPq).

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Authors and Affiliations

  1. Pediatric Nephrourology Unit, Department of Pediatrics, Hospital das Clínicas, Federal University of Minas Gerais, Rua Engenheiro Amaro Lanari 389/501, Belo Horizonte, MG, 30.310.580, Brazil

    Mariana A. Vasconcelos, Maria Candida F. Bouzada, Leticia R. Moura, Fabiana F. Santos, Juliana M. Oliveira, Flavia F. Carvalho, Ana Cristina Simões e Silva & Eduardo A. Oliveira

  2. Immunopharmacology Laboratory, Department of Pediatrics, Hospital das Clínicas, Federal University of Minas Gerais, Rua Engenheiro Amaro Lanari 389/501, Belo Horizonte, MG, 30.310.580, Brazil

    Katia D. Silveira & Mauro M. Teixeira

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  1. Mariana A. Vasconcelos
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  2. Maria Candida F. Bouzada
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  3. Katia D. Silveira
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Correspondence to Eduardo A. Oliveira.

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Vasconcelos, M.A., Bouzada, M.C.F., Silveira, K.D. et al. Urinary levels of TGF β-1 and of cytokines in patients with prenatally detected nephrouropathies. Pediatr Nephrol 26, 739–747 (2011). https://doi.org/10.1007/s00467-011-1802-4

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  • DOI: https://doi.org/10.1007/s00467-011-1802-4

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