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Investigation of deactivation thermodynamics of lipase immobilized on polymeric carrier

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Abstract

In the present work, we have investigated biochemical thermo-kinetic stability of lipases immobilized on a biocompatible polymeric material. Immobilization of lipase Candida rugosa (CRL) was carried out on biocompatible blend of poly vinyl alcohol (PVA) and chitosan (CHY) support via entrapment and glutardehyde (Glu) cross-linking method to produce PVA:CHY:CRL and PVA:CHY:Glu:CRL as robust biocatalyst. These immobilized lipases were characterized by various physico-biochemical characterization techniques. Later on, thermal and solvent stability of polymer immobilized lipase was determined in term of half-life time (t 0.5), D values, enthalpy (ΔH°), entropy (ΔS°), and free energy (ΔG°) of deactivation at different temperatures and in various solvents. The thermodynamic deactivation stability trend was found as: cross-linked lipase CRL > entrapped lipase CRL > free lipase CRL. Moreover, kinetic parameters, such as K m, V max, and catalytic efficiency, were also determined to understand the kinetic features. The polymer immobilized enzyme was reused to investigate the economic viability of the developed biocatalyst.

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Abbreviations

PVA:

Polyvinyl alcohol

CHY:

Chitosan

PVA:CHY:

Control matrix

PVA:CHY:Glu:

Activated glutaraldehyde matrix

PVA:CHY:CRL:

Entrapped lipase

PVA:CHY:CRL:Glu:

Immobilized cross-linked lipase

t 0.5 :

Half life time

K d :

Deactivation rate constant

D-value:

Decimal reduction value

ΔH°:

Enthalpy of deactivation

ΔG°:

Gibb’s free energy of deactivation

ΔS°:

Entropy of deactivation

Ea:

Activation energy

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Acknowledgements

Author Kirtikumar is grateful to the Council of Scientific & Industrial Research (CSIR), India (File No. 09/991(0015)/2011-EMR-I) for providing research fellowship and fund.

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Authors and Affiliations

  1. Department of Chemistry, Institute of Chemical Technology, Mumbai, 400019, India

    Kirtikumar C. Badgujar & Bhalchandra M. Bhanage

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  1. Kirtikumar C. Badgujar
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  2. Bhalchandra M. Bhanage
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Correspondence to Bhalchandra M. Bhanage.

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Badgujar, K.C., Bhanage, B.M. Investigation of deactivation thermodynamics of lipase immobilized on polymeric carrier. Bioprocess Biosyst Eng 40, 741–757 (2017). https://doi.org/10.1007/s00449-017-1740-z

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