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Antibacterial, anti-biofilm and anticancer potentials of green synthesized silver nanoparticles using benzoin gum (Styrax benzoin) extract

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Abstract

This study described a simple and green approach for the synthesis of silver nanoparticles (AgNPs) employing benzoin gum water extract as a reducing and capping agent and their applications. The AgNPs were characterized by ultraviolet–visible spectrophotometer, X-ray diffraction pattern, field emission transmission electron microscopy, dynamic light scattering, zeta potential and fourier transform infrared spectroscopy. The AgNPs showed promising antimicrobial activity against various pathogens (Gram-negative, Gram-positive and fungus) and possessed high free radical scavenging activity (104.5 ± 7.21 % at 1 mg/ml). In addition, the AgNPs exhibited strong cytotoxicity towards human cervical cancer and human lung cancer cells as compared to the normal mouse macrophage cells. Moreover, the AgNPs possessed anti-biofilm activity against Escherichia coli, and compatibility to human keratinocyte HaCaT cells, which suggests the use of dressing with the AgNPs in chronic wound treatment. Therefore, AgNPs synthesized by benzoin gum extract are comparatively green and may have broad spectrum potential application in biomedicine.

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References

  1. Zhang L, Wang X, Miao Y, Chen Z, Qiang P, Cui L, Jing H, Guo Y (2016) Magnetic ferroferric oxide nanoparticles induce vascular endothelial cell dysfunction and inflammation by disturbing autophagy. J Hazard Mater 304:186–195

    Article  CAS  Google Scholar 

  2. Lara HH, Garza-Trevino EN, Ixtepan-Turrent L, Singh DK (2011) Silver nanoparticles are broad-spectrum bactericidal and virucidal compounds. J Nanobiotechnol 9:30

    Article  CAS  Google Scholar 

  3. Jayaprakash N, Vijaya JJ, Kennedy LJ, Priadharsini K, Palani P (2015) Antibacterial activity of silver nanoparticles synthesized from serine. Mater Sci Eng C Mater Biol Appl 49:316–322

    Article  CAS  Google Scholar 

  4. Mishra PM, Sahoo SK, Naik GK, Parida K (2015) Biomimetic synthesis, characterization and mechanism of formation, of stable silver nano particles using Averrhoa carambola L. leaf extract. Mater Lett 160:566–571

    Article  Google Scholar 

  5. Otari SV, Patil RM, Ghosh SJ, Pawar SH (2014) Green phytosynthesis of silver nanoparticles using aqueous extract of Manilkara zapota (L.) seeds and its inhibitory action against Candida species. Mater Lett 116:367–369

    Article  CAS  Google Scholar 

  6. Lu Z, Rong K, Li J, Yang H, Chen R (2013) Size-dependent antibacterial activities of silver nanoparticles against oral anaerobic pathogenic bacteria. J Mater Sci Mater Med 24:1465–1471

    Article  CAS  Google Scholar 

  7. Xu R, Wang D, Zhang J, Li Y (2006) Shape-dependent catalytic activity of silver nanoparticles for the oxidation of styrene. Chem Asian J 1:888–893

    Article  CAS  Google Scholar 

  8. Ahamed M, Alsalhi MS, Siddiqui MK (2010) Silver nanoparticle applications and human health. Clin Chim Acta 411:1841–1848

    Article  CAS  Google Scholar 

  9. Danese PN (2002) Antibiofilm approaches: prevention of catheter colonization. Chem Biol 9:873–880

    Article  CAS  Google Scholar 

  10. Perez-Diaz M, Alvarado-Gomez E, Magana-Aquino M, Sanchez-Sanchez R, Velasquillo C, Gonzalez C, Ganem-Rondero A, Martinez-Castanon G, Zavala-Alonso N, Martinez-Gutierrez F (2016) Anti-biofilm activity of chitosan gels formulated with silver nanoparticles and their cytotoxic effect on human fibroblasts. Mater Sci Eng C Mater Biol Appl 60:317–323

    Article  CAS  Google Scholar 

  11. Clinton L, Carter T (2015) Chronic wound biofilms: pathogenesis and potential therapies. Labmedicine 46:277–284

    Google Scholar 

  12. Filippi JJ, Castel C, Fernandez X, Rouillard M, Gaysinski M, Lavoine-Hanneguelle S (2009) An unusual acenaphthylene-type sesquiterpene hydrocarbon from Siam and Sumatra benzoin gum. Phytochem Lett 2:216–219

    Article  CAS  Google Scholar 

  13. Castel C, Fernandez X, Lizzani-Cuvelier L, Loiseau AM, Perichet C, Delbecque C, Arnaudo JF (2006) Volatile constituents of benzoin gums: Siam and Sumatra, part 2. Study of headspace sampling methods. Flavour Fragr J 21:59–67

    Article  CAS  Google Scholar 

  14. Fernandez X, Lizzani-Cuvelier L, Loiseau A, Perichet C, Delbecque C (2003) Volatile constituents of benzoin gums: Siam and Sumatra. Part 1. Flavour Fragr J 18:328–333

    Article  CAS  Google Scholar 

  15. Eustis S, el-Sayed MA (2006) Why gold nanoparticles are more precious than pretty gold: noble metal surface plasmon resonance and its enhancement of the radiative and nonradiative properties of nanocrystals of different shapes. Chem Soc Rev 35:209–217

    Article  CAS  Google Scholar 

  16. Magudapathy P, Gangopadhyay P, Panigrahi BK, Nair KGM, Dhara S (2001) Electrical transport studies of Ag nanoclusters embedded in glass matrix. Phys B 299:142–146

    Article  CAS  Google Scholar 

  17. Kharat SN, Mendhulkar VD (2016) Synthesis, characterization and studies on antioxidant activity of silver nanoparticles using Elephantopus scaber leaf extract. Mater Sci Eng C Mater 62:719–724

    Article  CAS  Google Scholar 

  18. Xue M, Zhang X, Wu ZF, Wang H, Ding X, Tian XY (2013) Preparation and flame retardancy of polyurethane/POSS nanocomposites. Chin J Chem Phys 26:445–450

    Article  CAS  Google Scholar 

  19. Fan HB, Li XM, Liu YL, Yang RJ (2013) Thermal curing and degradation mechanism of polyhedral oligomeric octa(propargylaminophenyl)silsesquioxane. Polym Degrad Stabil 98:281–287

    Article  CAS  Google Scholar 

  20. Baicea CM, Luntraru VI, Vaireanu DI, Vasile E, Trusca R (2013) Composite membranes with poly(ether ether ketone) as support and polyaniline like structure, with potential applications in fuel cells. Cent Eur J Chem 11:438–445

    CAS  Google Scholar 

  21. Jiang FY, Wang XD, Wu DZ (2014) Design and synthesis of magnetic microcapsules based on n-eicosane core and Fe3O4/SiO2 hybrid shell for dual-functional phase change materials. Appl Energy 134:456–468

    Article  CAS  Google Scholar 

  22. Guan B, Latif PA, Yap T (2013) Physical preparation of activated carbon from sugarcane bagasse and corn husk and its physical and chemical characteristics. Int J Eng Res Sci Technol 2:1–14

    Google Scholar 

  23. Yang HP, Yan R, Chen HP, Lee DH, Zheng CG (2007) Characteristics of hemicellulose, cellulose and lignin pyrolysis. Fuel 86:1781–1788

    Article  CAS  Google Scholar 

  24. Rai M, Yadav A, Gade A (2009) Silver nanoparticles as a new generation of antimicrobials. Biotechnol Adv 27:76–83

    Article  CAS  Google Scholar 

  25. Jakubovics NS, Kolenbrander PE (2010) The road to ruin: the formation of disease-associated oral biofilms. Oral Dis 16:729–739

    Article  CAS  Google Scholar 

  26. Arunachalam KD, Arun LB, Annamalai SK, Arunachalam AM (2015) Potential anticancer properties of bioactive compounds of Gymnema sylvestre and its biofunctionalized silver nanoparticles. Int J Nanomed 10:31–41

    CAS  Google Scholar 

  27. Lin J, Huang ZH, Wu H, Zhou W, Jin PP, Wei PF, Zhang YJ, Zheng F, Zhang JQ, Xu J, Hu Y, Wang YH, Li YJ, Gu N, Wen LP (2014) Inhibition of autophagy enhances the anticancer activity of silver nanoparticles. Autophagy 10:2006–2020

    Article  Google Scholar 

  28. Ahmed KBA, Mahapatra SK, Raja MRC, Subramaniam S, Sengan M, Rajendran N, Das SK, Haldar K, Roy S, Sivasubramanian A, Anbazhagan V (2016) Jacalin-capped silver nanoparticles minimize the dosage use of the anticancer drug, shikonin derivatives, against human chronic myeloid leukemia. Rsc Adv 6:18980–18989

    Article  Google Scholar 

  29. Piao MJ, Kang KA, Lee IK, Kim HS, Kim S, Choi JY, Choi J, Hyun JW (2011) Silver nanoparticles induce oxidative cell damage in human liver cells through inhibition of reduced glutathione and induction of mitochondria-involved apoptosis. Toxicol Lett 201:92–100

    Article  CAS  Google Scholar 

  30. Banerjee J, Narendhirakannan RT (2011) Biosynthesis of silver nanoparticles from Syzygium cumini (L.) seed extract and evaluation of their in vitro antioxidant activities. Dig J Nanomater Biosci 6:961–968

    Google Scholar 

  31. Babu S, Velez A, Wozniak K, Szydlowska J, Seal S (2007) Electron paramagnetic study on radical scavenging properties of ceria nanoparticles. Chem Phys Lett 442:405–408

    Article  CAS  Google Scholar 

  32. Flora SJS (2007) Role of free radicals and antioxidants in health and disease. Cell Mol Biol 53:1–2

    CAS  Google Scholar 

  33. Niraimathi KL, Sudha V, Lavanya R, Brindha P (2013) Biosynthesis of silver nanoparticles using Alternanthera sessilis (Linn.) extract and their antimicrobial, antioxidant activities. Colloids Surf B Biointerfaces 102:288–291

    Article  CAS  Google Scholar 

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Acknowledgments

This work was conducted under the industrial infrastructure program (No. N0000888) for fundamental technologies which is funded by the Ministry of Trade, Industry & Energy (MOTIE, Korea).

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Authors and Affiliations

  1. Department of Oriental Medicinal Biotechnology, College of Life Science, Kyung Hee University, Global Campus, 1732 Deokyoungdaero, Giheung-gu, Yongin-si, Gyeonggi-do, 446-701, Republic of Korea

    Juan Du, Hina Singh & Tae-Hoo Yi

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  1. Juan Du
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  2. Hina Singh
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  3. Tae-Hoo Yi
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Correspondence to Tae-Hoo Yi.

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Du, J., Singh, H. & Yi, TH. Antibacterial, anti-biofilm and anticancer potentials of green synthesized silver nanoparticles using benzoin gum (Styrax benzoin) extract. Bioprocess Biosyst Eng 39, 1923–1931 (2016). https://doi.org/10.1007/s00449-016-1666-x

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  • DOI: https://doi.org/10.1007/s00449-016-1666-x

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