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Synthesis of a hybrid polymer-inorganic biomimetic support incorporating in situ pectinase from Aspergillus niger ATCC 9642

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Abstract

The hybrid alginate/gelatin/calcium oxalate (AGOCa) support was successfully synthesized through the biomimetic mineralization method for immobilization in situ of a pectinolytic extract from Aspergillus niger ATCC 9642 via entrapment technique. The efficiency of immobilization reached 72.7 %. Sodium oxalate buffer (100 mM, pH 5.5) was selected as adjuvant of the immobilization process by allowing the formation of a calcified shell around the calcium alginate capsule, significantly increasing the stability to storage, thermal and recycling of the enzymatic immobilized pectinolytic extract. The pH and temperature for maximum activity were from 5.0 to 6.0 and 60 to 80 °C, respectively. The new hybrid support can be a potential alternative to obtain immobilized pectinases with properties for advantageous industrial applications.

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References

  1. Pinelo M, Zeuner B, Meyer AS (2010) Juice clarification by protease and pectinase treatments indicates new roles of pectin and protein in cherry juice turbidity. Food Bioprod Process 88:259–265

    Article  CAS  Google Scholar 

  2. Sandri IG, Fontana RC, Barfknecht DM, Da Silveira MM (2011) Clarification of fruit juices by fungal pectinases. Lwt Food Sci Technol 44:2217–2222

    Article  CAS  Google Scholar 

  3. Uenojo M, Pastore GM (2007) Pectinolytic enzymes. Industrial applications and future perspectives. Quím Nova 30:388–394

    Article  CAS  Google Scholar 

  4. Seenuvasan M, Malar CG, Preethi S, Balaji N, Iyyappan J, Kumar MA, Kumar KS (2013) Immobilization of pectinase on co-precipitated magnetic nanoparticles for enhanced stability and activity. Res J Biotechnol 8:24–30

    CAS  Google Scholar 

  5. Abdelmajeed N, Kheli O, Danial E (2012) Immobilization technology for enhancing bio-products industry. Afr J Biotechnol 11:13528–13539

    Article  CAS  Google Scholar 

  6. Tischer W, Wedekind F (1999) Immobilized enzymes: methods and applications. Top Curr Chem 200:95–126

    Article  CAS  Google Scholar 

  7. Datta S, Christena L, Rajaram YR (2013) Enzyme immobilization: an overview on techniques and support materials. 3 Biotech 3:1–9

    Article  Google Scholar 

  8. Talbert JN, Goddart JM (2012) Enzymes on material surfaces. Colloid Surf B 93:8–19

    Article  CAS  Google Scholar 

  9. Lu Z, Zhang J, Ma Y, Song S, Gu W (2012) Biomimetic mineralization of calcium carbonate/carboxymethylcellulose. Mater Sci Eng 32:1982–1987

    Article  CAS  Google Scholar 

  10. Gao Q, Wang C, Tong Z (2010) Facile and high efficient fabrication of hybrid microcapsules for urease encapsulation and their use as biomimetic reactors. Res Chin Univ 26(5):842–846

    CAS  Google Scholar 

  11. Lai JK, Chuang TH, Jan JS, Wang SS (2010) Efficient and stable enzyme immobilization in a block copolypeptide vesicle-templated biomimetic silica support. Colloids Surf B 80:51–58

    Article  CAS  Google Scholar 

  12. Zhang L, Jiang Y, Jiang Z, Sun X, Shi J, Cheng W, Sun Q (2009) Immobilized transglucosidase in biomimetic polymer-inorganic hybrid capsules for efficient conversion of maltose to isomaltooligosaccharides. Biochem Eng J 46:186–192

    Article  CAS  Google Scholar 

  13. Lee JH, Kim SB, Park C, Kim SW (2010) Effect of a buffer mixture system on the activity of lipases during immobilization process. Biores Technol 101:S66–S70

    Article  CAS  Google Scholar 

  14. Gomes J, Zeni J, Cence K, Toniazzo G, Treichel H, Valduga E (2011) Evaluation of production and characterization of polygalacturonase by Aspergillus niger ATCC 9642. Food Bioprod Process 89:281–287

    Article  CAS  Google Scholar 

  15. Boanini E, Bigi A (2011) Biomimetic gelatin–octacalcium phosphate core–shell microspheres. J Colloid Interface Sci 362:594–599

    Article  CAS  Google Scholar 

  16. Shen Q, Yanga R, Hua X, Ye F, Zhang W, Zhao W (2011) Gelatin-templated biomimetic calcification for β-galactosidase immobilization. Process Biochem 46:1565–1571

    Article  CAS  Google Scholar 

  17. Miller GL (1959) Use of dinitrosalicyclic acid reagent for determination of reducing sugar. Anal Chem 37:426–428

    Article  Google Scholar 

  18. Al-Wahsh IA, Wu Y, Liebman M (2012) A comparison of two extraction methods for food oxalate assessment. J Food Res 1:233–239

    Article  CAS  Google Scholar 

  19. Hoffman AS (2012) Hydrogels for biomedical applications. Adv Drug Deliv Rev 64:18–23

    Article  Google Scholar 

  20. Pasparakis G, Bouropoulos N (2006) Swelling studies and in vitro release of verapamil from calcium alginate and calcium alginate–chitosan beads. Int J Pharm 323:34–42

    Article  CAS  Google Scholar 

  21. Ouwerx C, Velings N, Mestdagh M, Axelos M (1998) Physicochemical properties and rheology of alginate gel beads formed with various divalent cations. Polym Gels Netw 6:393–408

    Article  CAS  Google Scholar 

  22. Fundueanu G, Nastruzzi C, Carpov A, Desbrieres J, Rinaudo M (1999) Physico-chemical characterization of Ca-alginate microparticles produced with different methods. Biomaterials 20:1427–1435

    Article  Google Scholar 

  23. Yoo SH, Song YB, Chang PS, Lee HG (2006) Microencapsulation of α-tocopherol using sodium alginate and its controlled release properties. Int J Biol Macromol 38:25–30

    Article  CAS  Google Scholar 

  24. Zhang L, Jiang Y, Shi J, Sun X, Li J, Jiang Z (2008) Biomimetic polymer-inorganic hybrid microcapsules for yeast alcohol dehydrogenase encapsulation. React Funct Polym 68:1507–1515

    Article  CAS  Google Scholar 

  25. Reham HU, Aman A, Silipo A, Qader SA, Molinaro A, Ansari A (2013) Degradation of complex carbohydrate: immobilization of pectinase from Bacillus licheniformis KIBGE-IB21 using calcium alginate as a support. Food Chem 139:1081–1086

    Article  Google Scholar 

  26. Shukla S, Saxena S, Thakur J, Gupta R (2010) Immobilization of polygalacturonase from Aspergillus niger onto glutaraldehyde activated nylon-6 and its application in apple juice clarification. Acta Aliment Hung 39:277–299

    Article  CAS  Google Scholar 

  27. Li T, Li S, Wa N, Tain L (2008) Immobilization and stabilization of pectinase by multipoint attachment onto an activated agar-gel support. Food Chem 109:703–708

    Article  CAS  Google Scholar 

  28. Ramirez H, Briones A, Úbeda J, Arevalo M (2013) Immobilization of pectinase by adsorption on an alginate-coated chitin support. Biotechnol Appl 30:101–104

    CAS  Google Scholar 

  29. Pifferi P, Spagna G (1987) The immobilization of endopolygalacturonase on γ-alumina. J Mol Catal B Enzym 42:137–149

    Article  CAS  Google Scholar 

  30. Esawy MA, Gamal AA, Kamel Z, Ismail AS, Abdel-Fattah AF (2013) Evaluation of free and immobilized Aspergillus niger NRC1ami pectinase applicable in industrial processes. Carbohyd Polym 92:1463–1469

    Article  CAS  Google Scholar 

  31. Bahrami A, Hejazi P (2013) Electrostatic immobilization of pectinase on negatively charged AOT-Fe3O4 nanoparticles. J Mol Catal B Enzym 93:1–7

    Article  CAS  Google Scholar 

  32. Csanádi Z, Sisak C (2006) Immobilization of pectinex Ultra SP-L pectinase and its application to production of fructooligosaccharides. Acta Aliment Hung 35:205–212

    Article  Google Scholar 

  33. Wang B, Cheng F, Lu Y, Ge W, Zhang M, Yue B (2013) Immobilization of pectinase from Penicillium oxalicum F67 onto magnetic corn starch microspheres: characterization and application in juice production. J Mol Catal B Enzym 97:137–143

    Article  CAS  Google Scholar 

  34. Simões AS, Mori RY, Faria R, De Castro HF, Mendes AA (2011) Performance of hybrid matrix SiO2-chitosan to immobilize microbial lipase from Candida rugosa. Quim Nova 34:33–38

    Article  Google Scholar 

  35. Lei Z, Bi S (2007) The silica-coated chitosan particle from a layer-by-layer approach for pectinase immobilization. Enzym Microb Technol 40:1442–1447

    Article  CAS  Google Scholar 

  36. Li T, Wang N, Li S, Zhao Q, Guo M, Zhang C (2007) Optimization of covalent immobilization of pectinase on sodium alginate support. Biotechnol Lett 29:1413–1416

    Article  CAS  Google Scholar 

  37. Buga M, Ibrahim S, Nok A (2010) Physico-chemical characteristics of immobilized polygalacturonase from Aspergillus niger (AS6). Afr J Biotechnol 9:8934–8943

    CAS  Google Scholar 

  38. Spagna G, Pifferi P, Tramontini M (1995) Immobilization and stabilization of pectinlyase on sinthetic polymers for application in the beverage industry. J Mol Catal B Enzym 101:99–105

    Article  CAS  Google Scholar 

  39. Wu R, He B, Zhao G, Li X (2014) Immobilization of pectinase on polyethyleneimine-coated pulp fiber for treatment of whitewater from papermaking. J Mol Catal B Enzym 99:163–168

    Article  CAS  Google Scholar 

  40. Won K, Sangbum K, Kwang-Je K, Hong P, Sang-Ji M (2005) Optimization of lipase entrapment in Ca-alginate gel bead. Process Biochem 40:2149–2154

    Article  CAS  Google Scholar 

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Acknowledgments

The authors thank URI-Erechim, CNPq, FAPERGS and CAPES for the infrastructure and financial support.

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Authors and Affiliations

  1. Departamento de Ciências Agrárias, Universidade Regional do Alto Uruguai e das Missões, URI-Erechim, Erechim, RS, 99700-000, Brazil

    Cindy Elena Bustamante-Vargas, Marcelo Luis Mignoni, Luciana Dornelles Venquiaruto, Eunice Valduga, Geciane Toniazzo & Rogério Marcos Dallago

  2. Departamento de Engenharia Química e de Alimentos, Universidade Federal de Santa Catarina, UFSC, PO Box 476, Florianópolis, SC, 88040-900, Brazil

    Débora de Oliveira

Authors
  1. Cindy Elena Bustamante-Vargas
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  2. Marcelo Luis Mignoni
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  3. Débora de Oliveira
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  4. Luciana Dornelles Venquiaruto
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  5. Eunice Valduga
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  6. Geciane Toniazzo
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  7. Rogério Marcos Dallago
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Correspondence to Débora de Oliveira.

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Bustamante-Vargas, C.E., Mignoni, M.L., de Oliveira, D. et al. Synthesis of a hybrid polymer-inorganic biomimetic support incorporating in situ pectinase from Aspergillus niger ATCC 9642. Bioprocess Biosyst Eng 38, 1569–1577 (2015). https://doi.org/10.1007/s00449-015-1399-2

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  • DOI: https://doi.org/10.1007/s00449-015-1399-2

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