Abstract
A novel bionanocomposite, horse radish peroxidase- gold-nanoparticle–Calcium carbonate (HRP-AuNPs-CaCO3), hybrid material was encapsulated by silica sol on a glassy carbon electrode (GCE). The fabricated modified electrode was used as a novel voltammetric sensor for electrochemical sensing of anti-HIV replication drug i.e. deferiprone. The surface morphology of the modified electrode was characterized by scanning electron microscopy (SEM). Results obtained from the voltammetric measurements show that HRP-AuNPs-CaCO3 modified GCE offers a selective and sensitive electrochemical sensor for the determination of deferiprone. Under experimental conditions, the proposed voltammetric sensor has a linear response range from 0.01 to 10,000 μM with a detection limit of 0.01 μM. Furthermore, the fabricated sensor was successfully applied to determine deferiprone level in spiked urine and serum samples.
Similar content being viewed by others
Change history
11 March 2019
The Figs.��2, 4 and 5 were published wrongly in this paper, due to inadvertent compilation of figures during uploading the paper.
11 March 2019
The Figs.��2, 4 and 5 were published wrongly in this paper, due to inadvertent compilation of figures during uploading the paper.
References
Cohen MS, Hellmann N, Levy JA, DeCock K, Lange (2008) The spread, treatment, and prevention of HIV-1: evolution of a global pandemic. J Clin Invest 118:1244
Grady RW, Giardina PJ (2000) Iron chelation: rationale for combination therapy. In: Dadman DG, Bergeron RJ, Brittenham GM (eds) Iron Chelators: new development strategies. Ponte VedraBeach, Saratoga, p 293
Kontoghiorghes GJ, Pattichis K, Neocleous K, Kolnagou A (2004) The design and development of deferiprone (L1) and other iron chelators for clinical use: targeting methods and application prospects. Curr Med Chem 11:2161
Kontoghiorghes GJ, Barlett AN, Hoffbrand AV (1990) Long-term trial with the oral iron chelator 1,2-dimethyl-3-hydroxypyrid-4-one (L1) I. iron chelation and metabolic studies. Br J Haematol 76:301
Refaie FN, Wonke B, Hoffbrand AV, Wickens DG, Nortey P, Kontoghiorghes GJ (1992) Efficacy and possible adverse effects of the oral iron chelator 1, 2-dimethyl-3-hydroxypyrid-4-one (L1) in thalassemia major. Blood 80:593
Epemolu RO, Singh S, Hider RC, Damani LA (1990) Chromatography of 3-hydroxypyridin-4-ones: novel orally active iron chelators. J Chromatogr 519:171
Goddard JG, Kontoghiorghes G (1990) Chromatography of 3-hydroxypyridin-4-ones: novel orally active iron chelators. J Clin Chem 36:8
Biancamano MO, Connelly J, Villeneuve L, Caron P, Guillemette C (2009) Deferiprone glucuronidation by human tissues and recombinant UDP glucuronosyltransferase 1A6: an in vitro investigation of genetic and splice variants. Drug Meta Disp 37:329
Wang J, Chen G, Jiang H, Li Z, Wang X (2013) Advances in nano-scaled biosensors for biomedical applications. Analyst 138:4427
Narang J, Chauhan N, Pundir CS (2011) A non-enzymatic sensor for hydrogen peroxide based on polyaniline, multiwalled carbon nanotubes and gold nanoparticles modified Au electrode. Analyst 136:1938
Baghayeria M, Zare EN, Lakouraj MM (2014) A simple hydrogen peroxide biosensor based on a novel electro-magnetic poly (p-phenylenediamine)@Fe3O4 nanocomposite. Biosens Bioelectron 55:259
Baghayeri M, Zare EN, Hasanzadeh R (2014) Facile Synthesis of PSMA-g 3ABA/MWCNTs nanocomposite as a substrate for hemoglobin immobilization: application to catalysis of H2O2. Mater Sci Eng, C 39:213
Baghayeri M, Veisi H, Maleki B, Karimi Maleh H, Beitollahi H (2014) Multi-walled carbon nanotubes decorated with palladium nanoparticles as a novel platform for electrocatalytic sensing applications. RSC Advances 4:49595
Baghayeri M, Zare EN, Novel MM (2014) Superparamagnetic PFu@Fe3O4 conductive nanocomposite as a suitable host for hemoglobin immobilization. Sens Actuators B Chem 202:1200
Baghayeri M, Maleki B, Zarghani R (2014) Voltammetric behavior of tiopronin on carbon paste electrode modified with nanocrystalline Fe50Ni50 alloys. Mater Sci Eng C 44:175
Baghayeri M, Zare EN, Namadchian M (2013) Direct electrochemistry and electrocatalysis of hemoglobin immobilized on biocompatible poly (styrene-alternative-maleic acid)/functionalized multi-wall carbon nanotubes blends. Sens Actuators B Chem 188:227
Chauhan N, Singh A, Narang J, Dahiya S, Pundir CS (2012) Development of amperometric lysine biosensors based on Au nanoparticles/multiwalled carbon nanotubes/polymers modified Au electrodes. Analyst 20:1
Rosu R, Iwasaki R, Shimizu N, Doisaki N, Yamane T (1998) Intensification of lipase performance in a transesterification reaction by immobilization on CaCO3 powder. J Biotechnol 66:51
Xu Q, Cai WY, Zhu JJ, Zhao XN, Chen HY (2006) Porous gold-nanoparticle-CaCO3 hybrid material: preparation, characterization, and application for horseradish peroxidase assembly and direct electrochemistry. Chem matt 18:279
Shchukin D, Shutava T, Sukhorukov G, Lvov YM (2004) Modified polyelectrolyte microcapsules as smart defense systems. Chem Mater 16:3446
Volodkin DV, Larionova NI, Sukhorukov GB (2004) Protein encapsulation via porous CaCO3 microparticles templating. Biomacromol 5:1962
Wang Q, Lu G, Yang B (2004) Direct electrochemistry and electrocatalysis of hemoglobin immobilized on carbon paste electrode by silica sol-gel film. Biosens Bioelectron 19:1269
Chauhan N, Narang J, Pundir CS (2011) Immobilization of rat brain acetylcholinesterase on porous gold-nanoparticle–CaCO3 hybrid material modified Au electrode for detection of organophosphorous insecticides. Int J Biol Macromol 49:923–929
Xie C, Li H, Li S, Wu J, Zhang Z (2010) Surface molecular self-assembly for organophosphate pesticide imprinting in electropolymerized poly (p-aminothiophenol) membranes on a gold nanoparticles. Anal Chem 82:241
Yadegari H, Jabbari A, Heli H, Moosavi-Movahedi AA, Karimian K, Chem (2008) Electro-oxidation and determination of deferiprone on a glassy carbon electrode. Anal (Warsaw) 53:5–28
Bard AJ, Faulkner LR, (2001) Electrochemical methods: fundamentals and application ed. Wiley, New York, p 236
Yadegari H, Jabbari A, Heli H, Moosavi-Movahedi AA, Majdia S (2008) Electrooxidation and determination of etidronate using copper nanoparticles and microparticles-modified carbon paste electrodes. J Braz Chem Soc 19:1017
Lashkara JM, Amjadia M, Soleymani J, Tamizi E, Panahi-Azard V, Jouybane A (2012) Development and validation of a terbium-sensitized luminescence analytical method for deferiprone. I J Pharma Res 11:771
Yadegari H, Jabbari A, Heli H, Moosavi-Movahedi AA, Karimian K, Khodadadi A (2008) Electrocatalytic oxidation of deferiprone and its determination on a carbon nanotube-modified glassy carbon electrode. Electrochim Acta 53:2907
Hajjizadeh M, Jabbari A, Heli H, Moosavi-Movahedi AA, Shafiee A, Karimian K (2008) Electrocatalytic oxidation and determination of deferasirox and deferiprone on a nickel oxyhydroxide-modified electrode. Anal Biochem 373:337
Acknowledgments
In the present work, one of the author (Jagriti Narang) was supported by SERB, Department of Science and Technology (DST), India. Thanks to all scientists referenced throughout the paper whose valuable work has guided the way through to this research work.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Narang, J., Malhotra, N., Singh, G. et al. Voltammetric detection of anti-HIV replication drug based on novel nanocomposite gold-nanoparticle–CaCO3 hybrid material. Bioprocess Biosyst Eng 38, 815–822 (2015). https://doi.org/10.1007/s00449-014-1323-1
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00449-014-1323-1