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Continuous degradation of maltose: improvement in stability and catalytic properties of maltase (α-glucosidase) through immobilization using agar-agar gel as a support

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Abstract

Maltose degrading enzyme was immobilized within agar-agar support via entrapment method due to its industrial utilization. The maximum immobilization efficiency (82.77 %) was achieved using 4.0 % agar-agar keeping the diameter of bead up to 3.0 mm. The matrix entrapment showed maximum catalytic activity at pH 7.0 and temperature 65 °C. Substrate saturation kinetics showed that the K m of immobilized enzyme increased from 1.717 to 2.117 mM ml−1 where as Vmax decreased from 8,411 to 7,450 U ml−1 min−1 as compared to free enzyme. The immobilization significantly increased the stability of maltase against various temperatures and immobilized maltase retain 100 % of its original activity after 2 h at 50 °C, whereas the free maltase only showed 60 % residual activity under same condition. The reusability of entrapped maltase showed activity up to 12 cycles and retained 50 % of activity even after 5th cycle. Storage stability of agar entrapped maltase retain 73 % of its initial activity even after 2 months when stored at 30 °C while free enzyme showed only 37 % activity at same storage conditions.

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Authors and Affiliations

  1. The Karachi Institute of Biotechnology and Genetic Engineering (KIBGE), University of Karachi, Karachi, 75270, Pakistan

    Muhammad Asif Nawaz, Asad Karim, Afsheen Aman & Shah Ali Ul Qader

  2. Department of Chemical Sciences, University of Napoli Federico II, Complesso Universitario Monte Santangelo, Via Cintia 4, 80126, Naples, Italy

    Roberta Marchetti & Antonio Molinaro

Authors
  1. Muhammad Asif Nawaz
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  2. Asad Karim
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  3. Afsheen Aman
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  4. Roberta Marchetti
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  5. Shah Ali Ul Qader
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  6. Antonio Molinaro
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Correspondence to Shah Ali Ul Qader.

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Nawaz, M.A., Karim, A., Aman, A. et al. Continuous degradation of maltose: improvement in stability and catalytic properties of maltase (α-glucosidase) through immobilization using agar-agar gel as a support. Bioprocess Biosyst Eng 38, 631–638 (2015). https://doi.org/10.1007/s00449-014-1302-6

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  • DOI: https://doi.org/10.1007/s00449-014-1302-6

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