Abstract
Particle size of lignocellulose materials is an important factor for enzymatic hydrolysis efficiency. In this study, corn stover was milled and sieved into different size fractions from 1.42, 0.69, 0.34, to 0.21 mm, and the corresponding enzymatic hydrolysis yields were 24.69, 23.96, 25.34, and 26.97 %, respectively. The results indicate that the hydrolysis yield is approximately constant with changing corn stover particle sizes in the experimental range. The overall surface area and the inner pore size measurement show that the overall specific surface area was less than 2 % with the half reduction of particle size due to the greater inner pore surface area. The scanning electron microscope photographs gave direct evidence of the much greater inner pore surface area of corn stover particles. This result provided a reference when a proper size reduction of lignocellulose materials is considered in biorefining operations.
Similar content being viewed by others
References
Mansfield SD, Mooney C, Saddler JN (1999) Substrate and enzyme characteristics that limit cellulose hydrolysis. Biotechnol Prog 15:804–816
Burns DS, Ooshima H, Converse AO (1989) Surface area of pretreated lignocellulosics as a function of the extent of enzymatic hydrolysis. Appl Biochem Biotechnol 20(21):79–94
Wong KKY, Deverell KF, Mackie KL, Clark TA, Donaldson LA (1988) The relationship between fiber porosity and cellulose digestibility in steam exploded Pinus radiata. Biotechnol Bioeng 31:447–456
Dasari RK, Berson RE (2007) The effect of particle size on hydrolysis reaction rates and rheological properties in cellulosic slurries. Appl Biochem Biotechnol 289:136–140
Pedersen M, Meyer AS (2009) Influence of substrate particle size and wet oxidation on physical surface structures and enzymatic hydrolysis of wheat straw. Biotechnol Prog 25:399–408
Yeha AI, Huanga YC, Chen SH (2010) Effect of particle size on the rate of enzymatic hydrolysis of cellulose. Carbohydr Polym 79:192–199
Rivers DB, Emert GH (1987) Lignocellulose pretreatment-a comparison of wet and dry ball attrition. Biotechnol Lett 9:365–368
Ballesteros I, Oliva JM, Negro MJ, Manzanares P, Ballesteros M (2002) Enzymatic hydrolysis of steam exploded herbaceous agricultural waste (Brassica carinata) at different particle sizes. Process Biochem 38:187–192
Nahzad MM, Ramos LP, Paszner L, Saddler JN (1995) Structural constraints affecting the initial enzymatic hydrolysis of recycled paper. Enzyme Microb Tech 17:68–74
Grous WR, Converse AD, Grethlein HE (1986) The effect of steam explosion pretreatment on pore size and enzymatic hydrolysis of poplar. Enzyme Microb Technol 8:274–280
Van Soest PJ, Robertson JB, Lewis BA (1991) Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. J Dairy Sci 74:3583–3597
Adney B, Baker J (1996) Measurement of cellulase activities. NREL, Golden, CO. LAP-006
Stone JE, Scallan AM, Donefer E, Ahlgren E (1969) Digestibility as a simple function of a molecule of a similar size to a cellulase enzyme. Adv Chem Ser 95:219–241
Zhu JY, Wang GS, Pan XJ, Gleisnera R (2009) Specific surface to evaluate the efficiencies of milling and pretreatment of wood for enzymatic saccharification. Chem Eng Sci 64:474–485
Modenbach AA, Nokes SE (2013) Enzymatic hydrolysis of biomass at high solids loadings––a review. Biomass Bioenergy 56:526–544
Acknowledgments
This research was supported by the Joint Training Program of Shanghai High School and ECUST, and the National Basic Research Program of China (2011CB707406), and the National High-Tech Program of China (2012AA022301/2014AA021901).
Author information
Authors and Affiliations
Corresponding author
Additional information
H. Li, C. Ye and K. Liu have contributed equally to this work.
Rights and permissions
About this article
Cite this article
Li, H., Ye, C., Liu, K. et al. Analysis of particle size reduction on overall surface area and enzymatic hydrolysis yield of corn stover. Bioprocess Biosyst Eng 38, 149–154 (2015). https://doi.org/10.1007/s00449-014-1253-y
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00449-014-1253-y