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Comparative survival analysis of 12 histidine kinase mutants of Deinococcus radiodurans after exposure to DNA-damaging agents

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Abstract

Bacteria are able to adapt to changes in the environment using two-component signal transduction systems (TCSs) composed of a histidine kinase (HK) and a response regulator (RR). Deinococcus radiodurans, one of the most resistant organisms to ionizing radiation, has 20 putative HKs and 25 putative RRs. In this study, we constructed 12 D. radiodurans mutant strains lacking a gene encoding a HK and surveyed their resistance to γ-radiation, UV-B radiation (302 nm), mitomycin C (MMC), and H2O2. Five (dr0860 −, dr1174 −, dr1556 −, dr2244 −, and dr2419 −) of the 12 mutant strains showed at least a one-log cycle reduction in γ-radiation resistance. The mutations (1) dr1174, dr1227, and dr2244 and (2) dr0860, dr2416, and dr2419 caused decreases in resistance to UV radiation and MMC, respectively. Only the dr2416 and dr2419 mutant strains showed higher sensitivity to H2O2 than the wild-type. Reductions in the resistance to γ-radiation and H2O2, but not to UV and MMC, were observed in the absence of DR2415, which seems to be a cognate RR of DR2416. This result suggests that DR2415/DR2416 (DrtR/S: DNA damage response TCS) may be another TCS responsible for the extreme resistance of D. radiodurans to DNA-damaging agents.

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Acknowledgments

This study was supported by Nuclear R&D program of Ministry of Education, Science and Technology (MEST), Republic of Korea.

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Authors and Affiliations

  1. Research Division for Biotechnology, Korea Atomic Energy Research Institute, Jeongeup, 580-185, Republic of Korea

    Seonghun Im, Dusup Song, Minho Joe, Dongho Kim & Sangyong Lim

  2. Interdisciplinary Program of Graduate School for Bioenergy and Biomaterials, Chonnam National University, Gwangju, 500-757, Republic of Korea

    Seonghun Im & Don-Hee Park

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  1. Seonghun Im
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  2. Dusup Song
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  4. Dongho Kim
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Correspondence to Sangyong Lim.

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Im, S., Song, D., Joe, M. et al. Comparative survival analysis of 12 histidine kinase mutants of Deinococcus radiodurans after exposure to DNA-damaging agents. Bioprocess Biosyst Eng 36, 781–789 (2013). https://doi.org/10.1007/s00449-013-0904-8

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  • DOI: https://doi.org/10.1007/s00449-013-0904-8

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