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Proteomic analysis of Spirogyra varians mutant with high starch content and growth rate induced by gamma irradiation

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Abstract

This study was conducted to develop a high-efficiency strain of Spirogyra varians for the production of biomass by radiation breeding. The characteristics of wild-type and mutant S. varians were analyzed through phenomenological and proteomic observations. The results of our phenomenological observations of the S. varians mutant demonstrated increases in growth rate and content of chlorophyll a, b, and a + b; in particular, a significant threefold increase was observed in starch accumulation. Proteomic analysis to investigate the differences in expression between wild-type and mutant proteins identified 18 proteins with significantly different expressions. From the literature review, it was confirmed that the up-regulated proteins were mainly involved in photosynthesis, carbohydrate biosynthesis, and energy metabolism. These results suggest the possibility of algae development by radiation breeding for the production of biofuel.

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References

  1. Ahloowalia BS, Maluszynski M (2001) Induced mutations—a new paradigm in plant breeding. Euphytica 118:167–173

    Article  CAS  Google Scholar 

  2. Baek D, Jin Y, Jeong JC, Lee HJ, Moon H, Lee J, Shin D, Kang CH, Kim DH, Nam J, Lee SY, Yun DJ (2008) Suppression of reactive oxygen species by glyceraldehyde-3-phosphate dehydrogenase. Phytochemistry 69:333–338

    Article  CAS  Google Scholar 

  3. Bischoff HW, Bold HC (1963) Phycological studies IV. Some soil algae from enchanted rock and related algal species. The University of Texas Publications, Austin, p 95

    Google Scholar 

  4. Caspar T, Huber SC, Somerville C (1985) Alterations in growth, photosynthesis, and respiration in a starchless mutant of Arabidopsis thaliana (L) deficient in chloroplast phosphoglucomutase activity. Plant Physiol 79:11–17

    Article  CAS  Google Scholar 

  5. Chen P, Min M, Chen Y, Wang L, Li Y, Chen Q, Wang C, Wan Y, Wang X, Cheng Y, Deng S, Hennessy K, Lin X, Liu Y, Wang Y, Martinez B, Ruan R (2009) Review of the biological and engineering aspects of algae to fuels approach. Int J Agric Biol Eng 2:1–30

    Google Scholar 

  6. Contreras L, Moenne A, Gaillard F, Potin P, Correa JA (2010) Proteomic analysis and identification of copper stress-regulated proteins in the marine alga Scytosiphon gracilis (Phaeophyceae). Aquat Toxicol 96:85–89

    Article  CAS  Google Scholar 

  7. Cross JM, von Korff M, Altmann T, Bartzetko L, Sulpice R, Gibon Y, Palacios N, Stitt M (2006) Variation of enzyme activities and metabolite levels in 24 Arabidopsis accessions growing in carbon-limited conditions. Plant Physiol 142:1574–1588

    Article  CAS  Google Scholar 

  8. Fischer R (1998) Isolation of mutants, a key for the analysis of complex pathways and for strain improvement. In: Verma A (ed) Microbes for health, wealth and sustainable environment. Malhotra Publishing House, New Delhi, pp 739–751

    Google Scholar 

  9. Grandbastien M (1998) Activation of plant retrotransposons under stress conditions. Trends Plant Sci 3:181–187

    Article  Google Scholar 

  10. Gibon Y, Blaesing OE, Palacios-Rojas N, Pankovic D, Hendriks JHM, Fisahn J, Hoehne M, Guenther M, Stitt M (2004) Adjustment of diurnal starch turnover to short days: depletion of sugar during the night leads to a temporary inhibition of carbohydrate utilization, accumulation of sugars and post-translational activation of ADP-glucose pyrophosphorylase in the following light period. Plant J 39:847–862

    Article  CAS  Google Scholar 

  11. Gouveia L, Oliveira AC (2009) Microalgae as a raw material for biofuels production. J Ind Microbiol Biotechnol 36:269–274

    Article  CAS  Google Scholar 

  12. Han JW, Lee KP, Yoon M, Kang SH, Kim GH (2009) Cold stress regulation of a bi-functional 3-dehydroquinate dehydratase/shikimate dehydrogenase (DHQ/SDH)-like gene in the freshwater green alga Spirogyra varians. Bot Mar 52:178–185

    Article  CAS  Google Scholar 

  13. Hancock JT, Henson D, Nyirenda M, Desikan R, Harrison J (2005) Proteomic identification of glyceraldehyde 3-phosphate dehydrogenase as an inhibitory target of hydrogen peroxide in Arabidopsis. Plant Physiol Biochem 43:828–835

    Article  CAS  Google Scholar 

  14. Horling F, Lamkemeyer P, König J, Finkemeier I, Kandlbinder A, Baier M, Dietz KJ (2003) Divergent light-, ascorbate-, and oxidative stress-dependent regulation of expression of the peroxiredoxin gene family in Arabidopsis. Plant Physiol 131:317–325

    Article  CAS  Google Scholar 

  15. Ilag LL, Kumar AM, Soll D (1994) Light regulation of chlorophyll biosynthesis at the level of 5-aminolevulinate formation in Arabidopsis. Plant Cell 6:265

    CAS  Google Scholar 

  16. Jiao S, Hilaire EJ, Guikema A (2004) Identification and differential accumulation of two isoforms of the CF1-β subunit under high light stress in Brassica rapa. Plant Physiol Bioch 42:883–890

    Article  CAS  Google Scholar 

  17. Martin C, Smith AM (1995) Starch biosynthesis. Plant cell 7:971–985

    CAS  Google Scholar 

  18. Meyer RC, Steinfath M, Lisec J, Becher M, Witucka-Wall H, Törjék O, Fiehn O, Eckardt A, Willmitzer L, Selbig J, Altmann T (2007) The metabolic signature related to high plant growth rate in Arabidopsis thaliana. Proc Natl Acad Sci USA 104:4759–4764

    Article  CAS  Google Scholar 

  19. Mohr G, Lambowitz AM (2003) Putative proteins related to group II intron reverse transcriptase/maturases are encoded by nuclear genes in higher plants. Nucleic Acids Res 31:647–652

    Article  CAS  Google Scholar 

  20. Molendijk AJ, Ruperti BK (2004) Palme Small GTPases in vesicle trafficking. Curr Opin Plant Biol 7:694–700

    Article  CAS  Google Scholar 

  21. Morrish TA, Gilbert N, Myers JS, Vincent BJ, Stamato TD, Taccioli GE, Batzer MA, Moran JV (2002) DNA repair mediated by endonuclease-independent LINE-1 retrotransposition. Nat Genet 31:159–165

    Article  CAS  Google Scholar 

  22. Ni Z, Kim ED, Ha M, Lackey E, Liu J, Zhang Y, Sun Q, Chen ZJ (2009) Altered circadian rhythms regulate growth vigour in hybrids and allopolyploids. Nature 457:327–331

    Article  CAS  Google Scholar 

  23. Niwa K, Hayashi Y, Abe T, Aruga Y (2009) Induction and isolation of pigmentation mutants of Porphyra yezoensis (Bangiales, Rhodophyta) by heavy-ion beam irradiation. Phycol Res 57:194–202

    Article  CAS  Google Scholar 

  24. Nowitzki U, Wyrich R, Westhoff P, Henze K, Schnarrenberger C, Martin W (1995) Cloning of the amphibolic Calvin cycle/OPPP enzyme o-ribulose-5-phosphate 3-epimerase (EC 5.1.3.1) from spinach chloroplasts: functional and evolutionary aspects. Plant Mol Biol 29:1279–1291

    Article  CAS  Google Scholar 

  25. Posewitz MC, Smolinski SL, Kanakagiri S, Melis A, Seibert M, Ghirardi ML (2004) Hydrogen photoproduction is attenuated by disruption of an isoamylase gene in Chlamydomonas reinhardtii. Plant Cell 16:2151–2163

    Article  CAS  Google Scholar 

  26. Smith AM, Zeeman SC (2006) Quantification of starch in plant tissues. Nat Protoc 1:1342–1345

    Article  CAS  Google Scholar 

  27. Stark DM, Timmerman KP, Barry GF, Preiss J, Kishore GM (1992) Regulation of the amount of starch in plant tissues by ADP glucose pyrophosphorylase. Science 258:287–292

    Article  CAS  Google Scholar 

  28. Suda Y, Yoshikawa T, Okuda Y, Tsunemoto M, Tanaka S, Ikeda K, Miyasaka H, Watanabe M, Sasaki K, Harada K (2009) Isolation and characterization of a novel antistress gene from Chlamydomonas sp. W80. J Biosci Bioeng 107:352–354

    Article  CAS  Google Scholar 

  29. Talapatra S, Wagner JD, Thompson CB (2002) Elongation factor-1 alpha is a selective regulator of growth factor withdrawal and ER stress-induced apoptosis. Cell Death Differ 9:856–861

    Article  CAS  Google Scholar 

  30. Tammam AA, Allam MM, Osman M (2005) Mutagenesis of Dunaliella salina. Int J Agric Biol 7:477–481

    CAS  Google Scholar 

  31. Tucker WC, Du Z, Hein R, Gromet-Elhanan Z, Richter ML (2001) Role of the ATP synthase α-subunit in conferring sensitivity to tentoxin. Biochemistry 40:7542–7548

    Article  CAS  Google Scholar 

  32. Ueda R, Hirayama S, Sugata K, Nakayama H (1996) Process for the production of ethanol from microalgae. US Patent 5,578,472

  33. Wang L, Pan ZY, Guo WW (2010) Proteomic analysis of leaves from a diploid cybrid produced by protoplast fusion between Satsuma mandarin and pummel. Plant Cell Tissue Organ Cult 103:165–174

    Article  CAS  Google Scholar 

  34. Wang SJ, Zheng YZ, Ma LB, Xu P, Zhu JY (2000) Gamma-rays induction of mutation in conchocelis of Porphyra yezoensis. Chin J Oceanol Limnol 18:47–53

    Article  Google Scholar 

  35. Wood ZA, Schröder E, Robin Harris J, Poole LB (2003) Structure, mechanism and regulation of peroxiredoxins. Trends Biochem Sci 28:32–40

    Article  CAS  Google Scholar 

  36. Zabawinski C, Van Den Koornhuyse N, D’Hulst C, Schlichting R, Giersch C, Delrue B, Lacroix J-M, Preiss J, Ball S (2001) Starchless mutants of Chlamydomonas reinhardtii lack the small subunit of a heterotetrameric ADP-glucose pyrophosphorylase. J Bacteriol 183:1069–1077

    Article  CAS  Google Scholar 

  37. Zeeman SC, Northrop F, Smith AM, Rees T (1998) A starch-accumulating mutant of Arabidopsis thaliana deficient in a chloroplastic starch-hydrolysing enzyme. Plant J 15:357–365

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, 580-185, South Korea

    Minchul Yoon & Dong-Ho Kim

  2. Department of Biology, Kongju National University, Kongju, 314-701, South Korea

    Gwang Hoon Kim

  3. Department of Biotechnology and Bioengineering, Chonnam National University, Gwangju, 500-757, South Korea

    Jong-il Choi & Don-Hee Park

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  1. Minchul Yoon
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  2. Jong-il Choi
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  3. Gwang Hoon Kim
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  4. Dong-Ho Kim
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Correspondence to Jong-il Choi.

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Yoon, M., Choi, Ji., Kim, G.H. et al. Proteomic analysis of Spirogyra varians mutant with high starch content and growth rate induced by gamma irradiation. Bioprocess Biosyst Eng 36, 765–774 (2013). https://doi.org/10.1007/s00449-013-0902-x

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  • DOI: https://doi.org/10.1007/s00449-013-0902-x

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