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Efficient production of 1,3-propanediol from glycerol upon constitutive expression of the 1,3-propanediol oxidoreductase gene in engineered Klebsiella pneumoniae with elimination of by-product formation

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Abstract

In the present study, we developed an efficient method of 1,3-propanediol (1,3-PD) production from glycerol by genetic engineering of Klebsiella pneumoniae AK mutant strains. The proposed approach eliminated by-product formation and IPTG induction resulted in maximal production of 1,3-PD. A series of recombinant strains was designed to constitutively express the dhaB and/or dhaT genes, using the bacteriophage T5 PDE20 promoter and the rho-independent transcription termination signal of the Rahnella aquatilis levansucrase gene. Among these strains, AK/pConT expressing dhaT alone gave the highest yield of 1,3-PD. Fed-batch fermentation resulted in efficient production of 1,3-PD from either pure or crude glycerol, without by-product formation.

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Acknowledgments

This subject was supported by Korea Ministry of Environment as “Converging technology project” and by the New & Renewable Energy Technology Development Program of the Korea Institute of Energy Technology Evaluation and Panning (KETEP) Grant (2010T100100690) funded by the Korea government Ministry of Knowledge Economy.

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Authors and Affiliations

  1. Microbe-based Fusion Technology Research Center, Jeonbuk Branch Institute, KRIBB, Jeongeup, Jeonbuk, 580-185, South Korea

    Baek-Rock Oh, Jeong-Woo Seo, Sun-Yeon Heo, Lian Hua Luo, Won-Kyung Hong, Don-Hee Park & Chul-Ho Kim

  2. Interdisciplinary Program of Graduate School for Bioenergy and Biomaterials, Chonnam National University, Gwangju, 500-757, South Korea

    Baek-Rock Oh & Don-Hee Park

  3. Research Center of Bioactive Materials, Institute for Molecular Biology and Genetics, Chonbuk National University, Jeonju, Chonbuk, 561-756, South Korea

    Lian Hua Luo

Authors
  1. Baek-Rock Oh
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  2. Jeong-Woo Seo
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  3. Sun-Yeon Heo
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  4. Lian Hua Luo
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  5. Won-Kyung Hong
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  6. Don-Hee Park
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  7. Chul-Ho Kim
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Correspondence to Chul-Ho Kim.

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Oh, BR., Seo, JW., Heo, SY. et al. Efficient production of 1,3-propanediol from glycerol upon constitutive expression of the 1,3-propanediol oxidoreductase gene in engineered Klebsiella pneumoniae with elimination of by-product formation. Bioprocess Biosyst Eng 36, 757–763 (2013). https://doi.org/10.1007/s00449-013-0901-y

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  • DOI: https://doi.org/10.1007/s00449-013-0901-y

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