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Characterization of ethanol fermentation waste and its application to lactic acid production by Lactobacillus paracasei

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Abstract

In this study, an ethanol fermentation waste (EFW) was characterized for use as an alternative to yeast extract for bulk fermentation processes. EFW generated from a commercial plant in which ethanol is produced from cassava/rice/wheat/barley starch mixtures using Saccharomyces cerevisiae was used for lactic acid production by Lactobacillus paracasei. The effects of temperature, pH, and duration on the autolysis of an ethanol fermentation broth (EFB) were also investigated. The distilled EFW (DEFW) contained significant amounts of soluble proteins (2.91 g/l), nitrogen (0.47 g/l), and amino acids (24.1 mg/l). The autolysis of the EFB under optimum conditions released twice as much amino acids than in the DEFW. Batch fermentation in the DEFW increased the final lactic acid concentration, overall lactic acid productivity, and lactic acid yield on glucose by 17, 41, and 14 %, respectively, in comparison with those from comparable fermentation in a lactobacillus growth medium (LGM) that contained 2 g/l yeast extract. Furthermore, the overall lactic acid productivity in the autolyzed then distilled EFW (ADEFW) was 80 and 27 % higher than in the LGM and DEFW, respectively.

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Acknowledgments

This work was supported by the Industrial Strategic Technology Development Program (No. 10035241) and funded by the Ministry of Knowledge Economy (MKE, Korea). This study was also supported by the Energy Efficiency & Resources Program (No. 201010094C) of the Korea Institute of Energy Technology Evaluation and Planning (KETEP) grant funded by the MKE.

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Authors and Affiliations

  1. Changhae Institute of Cassava and Ethanol Research, Changhae Ethanol Co., Jeonju, 561-203, Republic of Korea

    Se-Kwon Moon & Gi-Wook Choi

  2. Research and Development Center, GS Caltex Corporation, Daejeon, 305-380, Republic of Korea

    Julia Lee, Hyohak Song, Jung-Hee Cho & Doyoung Seung

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  1. Se-Kwon Moon
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  2. Julia Lee
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  3. Hyohak Song
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Correspondence to Hyohak Song.

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S.-K. Moon and J. Lee contributed equally to this work.

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Moon, SK., Lee, J., Song, H. et al. Characterization of ethanol fermentation waste and its application to lactic acid production by Lactobacillus paracasei . Bioprocess Biosyst Eng 36, 547–554 (2013). https://doi.org/10.1007/s00449-012-0810-5

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  • DOI: https://doi.org/10.1007/s00449-012-0810-5

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