Abstract
The effects of granules-inocula on the start-up of anaerobic reactors treating dairy manure were studied in a batch-fed reactor. The effects of start-up period and ratio of granules to feed were analyzed. Results indicated that the effects of start-up period could be described by Langmuir model, while the Extended Freundlich model could be used to model the effects of ratio of granules to feed on cumulative biogas production. In addition, transmission electron microscopes (TEM) and scanning electron microscope analysis were conducted to elucidate the distribution of microbial population and micro-colonies in granules and manure. From the TEM micrographs analyses, the ratios the Syntrophobacter and methanogens in granule and manure were shown to be 1.57 ± 0.42 and 0.22 ± 0.20, respectively. These results demonstrated that granules-inocula could reduce the period required for onset of biogas by 25%.
References
USDA-SCS (1992) Agricultural waste management field handbook. United States Department of Agriculture, Washington, DC
Griffin ME, McMahon KD, Mackie RI, Raskin L (1998) Methanogenic population dynamics during start-up of anaerobic digesters treating municipal solid waste and biosolids. Biotechnol Bioeng 57:342–355
Show KY, Wang Y, Foong SF, Tay JH (2004) Accelerated start-up and enhanced granulation in upflow anaerobic sludge blanket reactors. Water Res 38:2293–2304
Vavilin VA, Angelidaki I (2005) Anaerobic degradation of solid material: importance of initiation centers for methanogenesis, mixing intensity, and 2D distributed model. Biotechnol Bioeng 89:113–122
McMahon KD, Stroot PG, Mackie RI, Raskin L (2001) Anaerobic codigestion of municipal solid waste and biosolids under various mixing conditions—II: microbial population dynamics. Water Res 35:1817–1827
Fang HHP (2000) Microbial distribution in UASB granules and its resulting effects. Water Sci Technol 42:201–208
Schmidt JE, Ahring BK (1996) Granular sludge formation in upflow anaerobic sludge blanket (UASB) reactors. Biotechnol Bioeng 49:229–246
Lettinga G, Van Velsen AFM, Hobma SW, Zeeuw W, Klapwijk A (1980) Use of the upflow sludge blanket (USB) reactor concept for biological wastewater treatment, especially for anaerobic treatment. Biotechnol Bioeng 22:699–734
Hulshoff Pol LW, De Castro Lopes SI, Lettinga G, Lens PN (2004) Anaerobic sludge granulation. Water Res 38:1376–1389
Baloch MI, Akunna JC, Kierans M, Collier PJ (2008) Structural analysis of anaerobic granules in a phase separated reactor by electron microscopy. Bioresour Technol 99:922–929
Baloch MI, Akunna JC, Collier PJ (2007) The performance of a phase separated granular bed bioreactor treating brewery wastewater. Bioresour Technol 98:1849–1855
Batstone DJ, Keller J, Blackall LL (2004) The influence of substrate kinetics on the microbial community structure in granular anaerobic biomass. Water Res 38:1390–1404
Bhatti ZI, Furkukawa K, Fujita M (1995) Comparative composition and characteristics of methanogenic granular sludges treating industrial wastes under different conditions. J Ferment Bioeng 79:273–280
Cruz CCV, Da Costa ACA, Henriques CA, Luna AS (2004) Kinetic modeling and equilibrium studies during cadmium biosorption by dead Sargassum sp. biomass. Bioresour Technol 91:249–257
Sibbesen E (1981) Some new equations to describe phosphate sorption by soils. J Soil Sci 32:67–74
Hussein H, Ibrahim SF, Kandeel K, Moawad H (2004) Biosorption of heavy metals from waste water using Pseudomonas sp. Electron J Biotechnol 7:30–37
Dianati-Tilaki RA (2003) Study on removal of cadmium from water environment by adsorption on GAC, BAC, and biofilter. In: Proceeding of the 7th international conference—diffuse pollution and basin management, Dublin, Ireland
Guibal E, Saucedo I, Jansson-Charrier M, Delanghe B, Cloirec P (1994) Uranium and vanadium sorption by chitosan and derivatives. Water Sci Technol 30:183–190
Varzakas T, Arapoglou D, Israilides C (2006) Kinetics of endoglucanase and endoxylanase uptake by soybean seeds. J Biosci Bioeng 101:111–119
Dianati-Tilaki RA, Mahvi AH, Shariat M, Nasseri S (2004) Study of cadmium removal from environmental water by biofilm covered granular activated carbon. Iranian J Publ Health 33:43–52
APHA (1995) Standard methods for the examination of water and wastewater, 19th edn. American Public Health Association, American Water Works Association, Water Environmental Federation, Washington, DC
Howgrave-Graham AR, Wallis FM (1993) Quantification of bacterial morphotypes within anaerobic digester granules from transmission electron micrographs using image analysis. Biotechnol Tech 7:143–148
Dudley BT, Howgrave-Graham AR, Bruton AG, Wallis FM (1993) The application of digital image processing to quantifying and measuring UASB digester granules. Biotechnol Bioeng 42:279–283
Benguella B, Benaissa H (2002) Cadmium removal from aqueous solution by chitin: kinetic and equilibrium studies. Water Res 36:2463–2474
Ratkowsky DA (1986) A statistical study of seven curves for describing the sorption of phosphate by soil. J Soil Sci 37:183–189
Lettinga G (1995) Anaerobic digestion and wastewater treatment systems. Antonie van Leeuwenhoek 67:3–28
McCarty PL (2001) The development of anaerobic treatment and its future. Water Sci Technol 44:149–156
Sekiguchi Y, Kamagata Y, Nakamura K, Syutsubo K, Ohashi A, Harada H, Nakamura K (1998) Diversity of mesophilic and thermophilic granular sludge determined by 16S rRNA gene analysis. Microbiology 22:2655–2665
Harmsen HJM, Akkermans ADL, Stams AJM, De Vos WM (1996) Population dynamics of propionate-oxidizing bacteria under methanogenic and sulfidogenic conditions in anaerobic granular sludge. Appl Environ Microbiol 62:2163–2168
Macleod FA, Guiot SR, Costerton JW (1990) Layered structure of bacterial aggregates produced in an upflow anaerobic sludge bed and filter reactor. Appl Environ Microbiol 56:1598–1607
Morvai L, Mihaltz P, Czako L, Hollo J (1991) Application of UASB-reactors in industrial wastewater treatment: performance data and results in granulation control. Acta Biotechnol 11:409–418
Wu W, Hickey RF, Zeikus JG (1991) Characterization of metabolic performance of methanogenic granules treating brewery wastewater: role of sulfate-reducing bacteria. Appl Environ Microbiol 57:3438–3449
Sow D, Ollivier B, Viaud P, Garcia JL (1989) Mesophilic and thermophilic methane fermentation of Euphorbia tirucalli. Microb Biotechnol 5:547–550
Fiebig K, Gottshalk G (1983) Methanogenesis from choline by coculture of Desulfovibrio spp. and Methanosarcina barki. Appl Environ Microbiol 45:161–168
Whitford MF, Teather RM, Forster RJ (2001) Phylogenetic analysis of methanogens from the bovine rumen. BMC Microbiol 1:5
Jarvis GN, Strompl C, Burgess DM, Skillman LC, Moore ER, Joblin KN (2000) Isolation and identification of ruminal methanogens from grazing cattle. Curr Microbiol 40:327–332
Stewart CS, Flint HJ, Bryant MP (1997) The rumen bacteria. In: Hobson PN, Stewart CS (eds) The rumen microbial ecosystem, 2nd edn. Blackie Academic and Professional, New York
McAllister TA, Okine EK, Mathison GW, Cheng KJ (1996) Dietary, environmental and microbiological aspects of methane production in ruminants. Can J Anim Sci 76:231–243
Bergen WG (2004) Quantitative determination of rumen ciliate protozoal biomass with real-time PCR. J Nutr 134:3223–3224
Tokura M, Chagan I, Ushida K, Kojima Y (1999) Phylogenetic study of methanogens associated with rumen ciliates. Curr Microbiol 39:123–128
Coleman GS (1979) Rumen ciliate protozoa. In: Levandowsky M, Hunter SH (eds) Biochemistry and physiology of protozoa, 2nd edn. Academic Press, New York
Samsoon PALNS, Loewenthal RE, Wentzel MC, Marais G (1990) Effect of nitrogen limitation on pelletization in upflow anaerobic sludge bed (UASB) systems. Water Res 16:165–170
Fang HHP (1997) Inhibition of bioactivity of UASB biogranules by electroplating metals. Pure Appl Chem 69:2425–2429
Stroot PG, McMahon KD, Mackie RI, Raskin L (2001) Anaerobic codigestion of municipal solid waste and biosolids under various mixing conditions-I: digester performance. Water Res 35:1804–1816
Lovely DR (1985) Minimum threshold for hydrogen metabolism in methanogenic bacteria. Appl Environ Microbiol 49:1530–1531
Shea TG, Pretorius WA, Cole RD, Pearson EA (1968) Kinetics of hydrogen assimilation in the methane fermentation. Water Res 2:833–848
Ejlertsson J, Karlsson A, Lagerkvist A, Hjertberg T, Svensson BH (2003) Effects of co-disposal of wastes containing organic pollutants with municipal solid waste—a landfill simulation reactor study. Adv Environ Res 7:949–960
Raposo F, Banks CJ, Siegert I, Heaven S, Borja R (2006) Influence of inoculum to substrate ratio on the biochemical methane potential of maize in batch tests. Process Biochem 41:1444–1450
Raposo F, Borja R, Martín MA, Martín A, de la Rubia MA, Rincón B (2009) Influence of inoculum–substrate ratio on the anaerobic digestion of sunflower oil cake in batch mode: process stability and kinetic evaluation. Chem Eng J 149:70–77
Liu Guangqing, Ruihong Z, El-Mashad HamedM, Renjie D (2009) Effect of feed to inoculum ratios on biogas yields of food and green wastes. Bioresour Technol 100:5103–5108
Gonzalez-Fernandez Cristina, Pedro A, Garcia-Encina (2009) Impact of substrate to inoculum ratio in anaerobic digestion of swine slurry. Biomass Bioenerg 33:1065–1069
Barlaz MA, Schaefer DM, Ham RK (1989) Bacterial population development and chemical characteristics of refuse decomposition in a simulated sanitary landfill. Appl Environ Microbiol 55:55–65
Karim K, Hoffmann R, Thomas Klasson K, Al-Dahhan MH (2005) Anaerobic digestion of animal waste: effect of mode of mixing. Water Res 39:3597–3606
Dubourguier HC, Archer DB, Albagnac G, Prensier G (1988) Structure and metabolism of methanogenic microbial conglomerates. In: Hall ER, Hobson PN (eds) Anaerobic digestion. Pergamon Press, Oxford
Acknowledgments
This study was conducted with financial support from Dr. Shulin Chen’s Research Program (Dr. Chen is a Professor of Biological System Engineering at Washington State University).
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Pandey, P.K., Ndegwa, P.M., Alldredge, J.R. et al. Modeling effects of granules on the start-up of anaerobic digestion of dairy wastewater with Langmuir and extended Freundlich equations. Bioprocess Biosyst Eng 33, 833–845 (2010). https://doi.org/10.1007/s00449-010-0406-x
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DOI: https://doi.org/10.1007/s00449-010-0406-x