Original Paper

Bioprocess and Biosystems Engineering

, Volume 32, Issue 6, pp 825-835

Production and characterization of lipopeptide biosurfactant by a sponge-associated marine actinomycetes Nocardiopsis alba MSA10

  • R. GandhimathiAffiliated withDepartment of Microbiology, Bharathidasan University
  • , G. Seghal KiranAffiliated withDepartment of Biotechnology, Bharathidasan University
  • , T. A. HemaAffiliated withDepartment of Microbiology, Bharathidasan University
  • , Joseph SelvinAffiliated withDepartment of Microbiology, Bharathidasan University Email author 
  • , T. Rajeetha RavijiAffiliated withDepartment of Microbiology, Bharathidasan University
  • , S. ShanmughapriyaAffiliated withDepartment of Microbiology, Bharathidasan University

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Abstract

A sponge-associated marine actinomycetes Nocardiopsis alba MSA10 was screened and evaluated for the production of biosurfactant. Biosurfactant production was confirmed by conventional screening methods including hemolytic activity, drop collapsing test, oil displacement method, lipase production and emulsification index. The active compound was extracted with three solvents including ethyl acetate, diethyl ether and dichloromethane. The diethyl ether extract was fractionated by TLC and semi-preparative HPLC to isolate the pure compound. In TLC, a single discrete spot was obtained with the R f 0.60 and it was extrapolated as valine. Based on the chemical characterization, the active compound was partially confirmed as lipopeptide. The optimum production was attained at pH 7, temperature 30°C, and 1% salinity with glucose and peptone supplementation as carbon and nitrogen sources, respectively. Considering the biosurfactant production potential of N. alba, the strain could be developed for large-scale production of lipopeptide biosurfactant.

Keywords

Biosurfactants Lipopeptide Marine actinomycetes Optimization Nocardiopsis