Bioprocess and Biosystems Engineering

, Volume 26, Issue 6, pp 385–391

Performance prediction of industrial centrifuges using scale-down models

  • M. Boychyn
  • S. S. S. Yim
  • M. Bulmer
  • J. More
  • D. G. Bracewell
  • M. Hoare
Original Paper

DOI: 10.1007/s00449-003-0328-y

Cite this article as:
Boychyn, M., Yim, S.S.S., Bulmer, M. et al. Bioprocess Biosyst Eng (2004) 26: 385. doi:10.1007/s00449-003-0328-y

Abstract

Computational fluid dynamics was used to model the high flow forces found in the feed zone of a multichamber-bowl centrifuge and reproduce these in a small, high-speed rotating disc device. Linking the device to scale-down centrifugation, permitted good estimation of the performance of various continuous-flow centrifuges (disc stack, multichamber bowl, CARR Powerfuge TM) for shear-sensitive protein precipitates. Critically, the ultra scale-down centrifugation process proved to be a much more accurate predictor of production multichamber-bowl performance than was the pilot centrifuge.

Keywords

CentrifugationScale-downShearClarification

List of symbols

BL

caulk width, m

C

centrifugation correction factor

Ca

Camp number

Clar

clarification, %

FL

correction factor for caulks on discs in centrifuge

mean velocity gradient, s−1

g

gravitational constant, m/s2

L

length, m

N

rotational speed, r/s

n

number of discs

Q

flow rate, m3/s

R

radius, m

r

radius, m

Re

Reynolds number

t

time, s

V

volume, m3

x

fractional acceleration time

y

fractional deceleration time

ZL

number of caulks on a disc in the centrifuge

Greek letters

ε

energy dissipation rate, W/kg

θ

half disc angle, rad

ρ

density, kg/m

Σ

equivalent settling area, m2

ω

angular velocity, rad/s

Subscripts

ds

disc-stack centrifuge

i

inner

L

liquid

mc

multichamber-bowl centrifuge

n

number

o

outer

ref

reference

tb

tubular-bowl centrifuge

Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • M. Boychyn
    • 1
    • 4
  • S. S. S. Yim
    • 1
  • M. Bulmer
    • 2
  • J. More
    • 2
  • D. G. Bracewell
    • 1
    • 3
  • M. Hoare
    • 1
    • 3
  1. 1.The Advanced Centre for Biochemical EngineeringUniversity College LondonLondonUK
  2. 2.Bio Products LaboratoryElstreeUK
  3. 3.The EPSRC IMRC for BioprocessingUniversity College LondonLondonUK
  4. 4.Eli Lilly and Co.IndianapolisUSA