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Immunolocalization and expression of Na+/K+ -ATPase in embryos, early larval stages and adults of the freshwater shrimp Palaemonetes argentinus (Decapoda, Caridea, Palaemonidae)

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Abstract

The euryhaline shrimp Palaemonetes argentinus exemplifies an evolutionary transition from brackish to freshwater habitats that requires adequate osmoregulatory capacities. Hyperosmoregulation is functional at hatching and it likely begins during the embryonic phase allowing this species to develop entirely in fresh water. Here, we investigated the Na+/K+-ATPase α-subunit gene (nka-α) expression using quantitative real-time PCR and localized Na+/K+-ATPase (NKA) in ion-transporting epithelia through immunofluorescence microscopy. We reared shrimps from spawning to juvenile stages at two salinities (1, 15 ‰) and maintained adults for 3 weeks at three salinity treatments (1, 15, 25 ‰). nka-α gene expression was measured in: (1) embryos at an early (SI), intermediate (SII) and late (SIII) stage of embryonic development; (2) newly hatched larvae (Zoea I, ZI); and (3) isolated gill tissue of adults. The nka-α expression was low in SI and SII embryos and reached maximum levels prior to hatching (SIII), which were similar to expression levels detected in the ZI. The nka-α expression in SIII and ZI was highest at 15 ‰, whereas salinity did not affect expression in earlier embryos. In SIII, in ZI and in a later zoeal stage ZIV, NKA was localized in epithelial cells of pleurae, in the inner-side epithelium of branchiostegite and in the antennal glands. Gills appeared in the ZIV but NKA immunolabeling of the cells of the gill shaft occurred in a subsequent developmental larval stage, the decapodid. Extrabranchial organs constitute the main site of osmoregulation in early ontogenetic stages of this freshwater shrimp.

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Acknowledgments

We wish to thank Ms E. Grousset for valuable assistance with classical histology and Ms E. Blondeau-Bidet for her technical help in molecular biology. This research was supported by PICT 0048–2010 from Agencia Nacional de Promoción Científica y Tecnológica (Argentina) and by a post-doctoral fellowship from Consejo Nacional de Investigaciones Científicas y Técnicas de la República Argentina (CONICET). RBI wishes to thank The Crustacean Society award (2008) and special thanks all the members of AEO team for their assistance.

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Authors and Affiliations

  1. Grupo Zoología Invertebrados, Instituto de Investigaciones Marinas y Costeras, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional de Mar del Plata, Dean Funes 3250, 7600, Mar del Plata, Argentina

    Romina Belén Ituarte & Eduardo Spivak

  2. Groupe Fonctionnel Adaptation Ecophysiologique et Ontogenèse, UMR 9190 MARBEC, UM-CNRS-IRD-Ifremer, Université Montpellier, cc 092, Place E. Bataillon, 34095, Montpellier cedex 05, France

    Jehan-Hervé Lignot, Guy Charmantier & Catherine Lorin-Nebel

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  1. Romina Belén Ituarte
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  2. Jehan-Hervé Lignot
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  3. Guy Charmantier
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  4. Eduardo Spivak
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  5. Catherine Lorin-Nebel
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Correspondence to Romina Belén Ituarte.

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Ituarte, R.B., Lignot, JH., Charmantier, G. et al. Immunolocalization and expression of Na+/K+ -ATPase in embryos, early larval stages and adults of the freshwater shrimp Palaemonetes argentinus (Decapoda, Caridea, Palaemonidae). Cell Tissue Res 364, 527–541 (2016). https://doi.org/10.1007/s00441-015-2351-0

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