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Interstitial cells of Cajal mediate excitatory sympathetic neurotransmission in guinea pig prostate

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Abstract

Morphological and functional studies have confirmed that interstitial cells of Cajal (ICCs) are involved in many enteric motor neurotransmission pathways. Recent investigations have demonstrated that human and guinea pig prostate glands possess a distinct cell type with morphological and immunological similarities to ICCs. These prostate ICCs have a close relationship with nerve bundles and smooth muscle cells. Prostate smooth muscle tone is largely induced by stimulation from the sympathetic nervous system, which releases excitatory norepinephrine (NE) to act on the α1-adrenoceptor. We have performed morphological and functional experiments to determine the role of ICCs in sympathetic neurotransmission in the guinea pig prostate based on the hypothesis that prostate ICCs act as mediators of sympathetic neurotransmission. Immunohistochemistry revealed many close points of contact between ICCs and sympathetic nerve bundles and smooth muscle cells. Double-labeled sections revealed that α1-adrenoceptor and the gap junction protein connexin 43 were expressed in prostate ICCs. Surprisingly, prostate ICCs co-expressed tyrosine hydroxylase and dopamine β-hydroxylase, two markers of sympathetic neurons. Functionally, the application of NE evoked a large single inward current in isolated prostate ICCs in a dose-dependent manner. The inward current evoked by NE was mediated via the activation of α1-adrenoceptors, because it was abolished by the non-specific α-adrenoceptor antagonist, phentolamine and the specific α1-adrenoceptor antagonist, prazosin. Thus, ICCs in the guinea pig prostate are target cells for prostate sympathetic nerves and possess the morphological and functional characteristics required to mediate sympathetic signals.

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Authors and Affiliations

  1. Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People’s Republic of China

    Jiang-ping Wang & Qin-zhang Wang

  2. Department of Urology, The First Affiliated Hospital of Medical College, Shihezi University, Shihezi, People’s Republic of China

    Jiang-ping Wang, Guo-fu Ding & Qin-zhang Wang

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  1. Jiang-ping Wang
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  2. Guo-fu Ding
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  3. Qin-zhang Wang
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Correspondence to Qin-zhang Wang.

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This work was funded by The National Natural Science Foundation of China, project no. 81060064.

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Wang, Jp., Ding, Gf. & Wang, Qz. Interstitial cells of Cajal mediate excitatory sympathetic neurotransmission in guinea pig prostate. Cell Tissue Res 352, 479–486 (2013). https://doi.org/10.1007/s00441-013-1572-3

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  • DOI: https://doi.org/10.1007/s00441-013-1572-3

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