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Control of grain protein contents through SEMIDWARF1 mutant alleles: sd1 increases the grain protein content in Dee-geo-woo-gen but not in Reimei

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Abstract

A new possibility for genetic control of the protein content of rice grains was suggested by the allele differences of the SEMIDWARF1 (SD1) mutation. Two quantitative trait loci—qPROT1 and qPROT12—were found on chromosomes 1 and 12, respectively, using backcrossed inbred lines of Sasanishiki/Habataki//Sasanishiki///Sasanishiki. One of them, qPROT1, increased almost all grain proteins instead of only certain proteins in the recessive Habataki allele. Fine mapping of qPROT1 revealed that two gene candidates—Os01g0883800 and Os01g0883900—were included in this region. Os01g0883800 encoded Gibberellin 20 oxidase 2 as well as SD1, the dwarf gene used in the so-called ‘Green Revolution’. Mutant analyses as well as sequencing analysis using the semi-dwarf mutant cultivars Dee-geo-woo-gen and Calrose 76 revealed that the sd1 mutant showed significantly higher grain protein contents than their corresponding wild-type cultivars, strongly suggesting that the high protein contents were caused by sd1 mutation. However, the sd1 mutant Reimei did not have high grain protein contents. It is possible to control the grain protein content and column length separately by selecting for sd1 alleles. From this finding, the genetic control of grain protein content, as well as the column length of rice cultivars, might be possible. This ability might be useful to improve rice nutrition, particularly in areas where the introduction of semi-dwarf cultivars is not advanced.

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Abbreviations

CAPS:

Cleaved amplified polymorphic sequence

CTAB:

Cetyl trimethyl ammonium bromide

GLM:

General linear model

LOD:

Logarithm of odds

NaOH:

Sodium hydroxide

PCR:

Polymerase chain reaction

QTL:

Quantitative trait locus

RFLP:

Restriction fragment length polymorphism

SDS:

Sodium dodecyl sulfate

SSR:

Simple sequence repeat

STS:

Sequence-tagged sites

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Acknowledgments

This work was partly supported by JSPS KAKENHI Grant Number 24580030. We are grateful to Mr. Tokuya Genba, Mr. Tuyoshi Kotake, Mr. Masanori Ichihashi, Mr. Shinobu Yuminamochi, Mr. Koushi Yazaki, Mr. Susumu Saitou, Mr. Ken-ichi Koide, Ms. Keiko Nozaki and Ms. Setsuko Hayashi for their excellent technical assistance.

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The authors declare that they have no conflicts of interest.

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Authors and Affiliations

  1. Hokuriku Research Center, NARO Agricultural Research Center, 1-2-1, Inada, Joetsu, Niigata, 943-0193, Japan

    Tomio Terao & Tatsuro Hirose

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  1. Tomio Terao
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  2. Tatsuro Hirose
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Correspondence to Tomio Terao.

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Communicated by S. Hohmann.

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Terao, T., Hirose, T. Control of grain protein contents through SEMIDWARF1 mutant alleles: sd1 increases the grain protein content in Dee-geo-woo-gen but not in Reimei. Mol Genet Genomics 290, 939–954 (2015). https://doi.org/10.1007/s00438-014-0965-7

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  • DOI: https://doi.org/10.1007/s00438-014-0965-7

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