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Identification, characterization and interaction of HAP family genes in rice

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Abstract

A HAP complex, which consists of three subunits, namely HAP2 (also called NF-YA or CBF-B), HAP3 (NF-YB/CBF-A) and HAP5 (NF-YC/CBF-C), binds to CCAAT sequences in a promoter to control the expression of target genes. We identified 10 HAP2 genes, 11 HAP3 genes and 7 HAP5 genes in the rice genome. All the three HAP family genes encode a protein with a conserved domain in each family and various non-conserved regions in both length and amino acid sequence. These genes showed various expression patterns depending on genes, and various combinations of overlapped expression of the HAP2, HAP3 and HAP5 genes were observed. Furthermore, protein interaction analyses showed interaction of OsHAP3A, a ubiquitously expressed HAP3 subunit of rice, with specific members of HAP5. These results indicate that the formation of specific complex with various HAP subunits combinations can be achieved by both tissue specific expression of three subunit genes and specific interaction of three subunit proteins. This may suggest that the HAP complexes may control various aspects of rice growth and development through tissue specific expression and complex formation of three subunit members.

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Acknowledgments

Full-length cDNAs were developed by the Rice Genome Project of the National Institute of Agrobiological Sciences, Japan, and provided from the Rice Genome Resource Center. We thank T. Makino for her technical assistance.

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Correspondence to Nori Kurata.

Additional information

Communicated by S. Hohmann.

Nucleotide sequence data reported are available in the DDBJ/EMBL/GenBank databases under the accession numbers AB288027 to AB288048 and BR000373 to BR000375.

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Thirumurugan, T., Ito, Y., Kubo, T. et al. Identification, characterization and interaction of HAP family genes in rice. Mol Genet Genomics 279, 279–289 (2008). https://doi.org/10.1007/s00438-007-0312-3

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  • DOI: https://doi.org/10.1007/s00438-007-0312-3

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