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The jmjN and jmjC domains of the yeast zinc finger protein Gis1 interact with 19 proteins involved in transcription, sumoylation and DNA repair

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Abstract

The jumonji domain is a highly conserved bipartite domain made up of two subdomains, jmjN and jmjC, which is found in many eukaryotic transcription factors. The jmjC domain was recently shown to possess the histone demethylase activity. Here we show that the jmjN and jmjC domains of the yeast zinc finger protein Gis1 interact in a two-hybrid system with 19 yeast proteins that include the RecQ helicase Sgs1, the silencing factors Esc1 and Sir4, the URI-type prefoldin Bud27 and the PIAS type SUMO ligase Nfi1/Siz2. Extensive interaction cross dependencies further suggest that the proteins form a larger complex. Consistent with this, 16 of the proteins also interact with a Bud27 two-hybrid bait, and three of them co-precipitate with TAP-tagged Gis1. The Gis1 jumonji domain can repress transcription when recruited to a promoter as a lexA fusion. This effect is dependent on both the jmjN and jmjC subdomains, as were all 19 two-hybrid interactions, indicating that the two subdomains form a single functional unit. The human Sgs1 homolog WRN also interacts with the Gis1 jumonji domain. Finally, we note that several jumonji domain interactors are related to proteins that are found in mammalian PML nuclear bodies.

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Acknowledgments

We thank Pernilla Bjerling, Stefan Björklund, Claes Gustafsson, Magnus Hallberg, Natalie von der Lehr, Ann Mutvei and Claudio de Virgilio for helpful advice and Roger Brent and Jan Olof Nehlin for generous gifts of strains and plasmids. This work was supported by grants from the Swedish Research Council, the Erik and Mai Pehrsson Foundation and the Agrifungen program at SLU.

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  1. Darius Balciunas

    Present address: Department of Genetics, Cell Biology and Development, University of Minnesota, 6-160 Jackson Hall, 321 Church St. SE, Minneapolis, MN, 55455, USA

Authors and Affiliations

  1. Department of Plant Biology and Forest Genetics, Swedish University of Agricultural Sciences, P.O. Box 7080, 75007, Uppsala, Sweden

    Susanna Tronnersjö, Christine Hanefalk, Darius Balciunas, Guo-Zhen Hu, Eva Murén & Hans Ronne

  2. Department of Medical Biochemistry and Microbiology, Uppsala University, P.O. Box 582, 75123, Uppsala, Sweden

    Susanna Tronnersjö, Darius Balciunas, Guo-Zhen Hu, Niklas Nordberg, Eva Murén & Hans Ronne

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  1. Susanna Tronnersjö
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Correspondence to Hans Ronne.

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Tronnersjö, S., Hanefalk, C., Balciunas, D. et al. The jmjN and jmjC domains of the yeast zinc finger protein Gis1 interact with 19 proteins involved in transcription, sumoylation and DNA repair. Mol Genet Genomics 277, 57–70 (2007). https://doi.org/10.1007/s00438-006-0171-3

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