Abstract
The Rf3 gene restores the pollen fertility disturbed by S male sterile cytoplasm. In order to develop molecular markers tightly linked to Rf3, we used amplified fragment length polymorphism (AFLP) technique with near isogenic lines (NILs) and bulk segregant analysis (BSA). A BC1F1 population from a pair of NILs with different Rf3 locus was constructed and 528 primer combinations was screened. A linkage map was constructed around the Rf3 locus, which was mapped on the distal region of chromosome 2 long arm with the help of SSR marker UMC2184. The closest marker E7P6 was 0.9 cM away from Rf3. Marker E3P1, 2.4 cM from Rf3, and E12M7, 1.8 cM from Rf3, were converted into a codominant CAPS and a dominant SCAR marker, and designated as CAPSE3P1 and SCARE12M7, respectively. These markers are useful for marker-assisted selection and map-based cloning of the Rf3 gene.
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Abbreviations
- AFLP:
-
Amplified fragment length polymorphism
- BF:
-
Fertile DNA bulks
- BS:
-
Sterile DNA bulks
- BSA:
-
Bulk segregant analysis
- CAPS:
-
Cleaved amplified polymorphic sequence
- CMS:
-
Cytoplasmic male sterility
- E/M :
-
EcoRI/MseI
- P/M :
-
PstI/MseI
- P/E :
-
EcoRI/PstI
- MAS:
-
Marker-assisted selection
- NILs:
-
Near isogenic lines
- ORF:
-
Open reading frames
- PC:
-
Primer combinations
- PPR:
-
Pentatricopeptide repeat
- RAPD:
-
Random amplified polymorphic DNA
- RFLP:
-
Restriction fragment length polymorphism
- SCAR:
-
Sequence characterized amplified regions
- SNP:
-
Singe nucleotide polymorphism
- SSR:
-
Simple sequence repeat, microsatellite
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Acknowledgments
The authors thank Prof. Neil Forsberg for critical review of this manuscript. This research was supported by the National High Technique Foundation of China “863” (2004AA222160), “948” Fund in Ministry of Agronomy of China (201010), the State Key Basic Research and Development Plan of China “973” (2001CB1088).
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Zhang, Z.F., Wang, Y. & Zheng, Y.L. AFLP and PCR-based markers linked to Rf3, a fertility restorer gene for S cytoplasmic male sterility in maize. Mol Genet Genomics 276, 162–169 (2006). https://doi.org/10.1007/s00438-006-0131-y
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DOI: https://doi.org/10.1007/s00438-006-0131-y