Skip to main content
SpringerLink
Log in

Description and phylogeny of a new microsporidium from the elm leaf beetle, Xanthogaleruca luteola Muller, 1766 (Coleoptera: Chrysomelidae)

  • Original Paper
  • Published:
Parasitology Research Aims and scope Submit manuscript

Abstract

This study describes a new genus and species of microsporidia which is a pathogen of the elm leaf beetle, Xanthogaleruca luteola Muller, 1776 (Coleoptera: Chrysomelidae). The beetles were collected from Istanbul in Turkey. All developmental stages are uninucleate and in direct contact with the host cell cytoplasm. Giemsa-stained mature spores are oval in shape and measured 3.40 ± 0.37 μm in length and 1.63 ± 0.20 μm in width. These uninucleate spores have an isofilar polar filament with 11 turns. The spore wall was trilaminar (75 to 115 nm) with a rugose, electron-dense exospore (34 to 45 nm) and a thickened, electron-lucent endospore (65 to 80 nm) overlaying the plasmalemma. Morphological, ultrastructural, and molecular features indicate that the described microsporidium is dissimilar to all known microsporidian taxa and confirm that it has different taxonomic characters than other microsporidia infecting X. luteola and is named here as Rugispora istanbulensis n. gen., n. sp.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Andreadis TG, Hanula JL (1987) Ultrastructural study and description of Ovavesicula popilliae N. G., N. Sp. (Microsporidia: Pleistophoridae) from the Japanese beetle, Popillia japonica (Coleoptera: Scarabaeidae). J Protozool 34:15–21. doi:10.1111/j.1550-7408.1987.tb03123.x

    Article  Google Scholar 

  • Balbiani EG (1882) Sur les microsporidies ou psorospermies des Articulés. C R Acad Sci Paris Ser D 95:1168–1171

    Google Scholar 

  • Becnel JJ, Andreadis TG (2014) Microsporidia in Insects. In: Microsporidia: pathogens of opportunity. 1st ed. John Wiley & Sons, Inc., pp 521–570.

  • Becnel JJ, Jeyaprakash A, Hoy MA, Shapiro A (2002) Morphological and molecular characterization of a new microsporidian species from the predatory mite Metaseiulus occidentalis (Nesbitt) (Acari, Phytoseiidae). J Invertebr Pathol 79:163–172. doi:10.1016/S0022-2011(02)00032-0

    Article  CAS  PubMed  Google Scholar 

  • Becnel JJ, Takvorian PM, Cali A (2014) Checklist of available generic names for microsporidia with type species and type hosts. In: Weiss LM, Becnel JJ (eds) Microsporidia: pathogens of opportunity. Wiley-Blackwell, USA, pp 671–687

    Google Scholar 

  • Boucias DG, Pendland JC (1998) Phylum Microsporidia. In: Principles of insect pathology. Springer Science + Business Media, Llc, New York, pp 399–414

    Chapter  Google Scholar 

  • Brooks WM, Becnel JJ, Kennedy GG (1988) Establishment of Endoreticulatus n. g. for Pleistophora fidelis (Hostounsky and Weiser, 1975) (Microsporidia: Pleistophoridae) based on the ultrastructure of a microsporidium in the Colorado potato beetle, Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae). J Protozool 35:481–488

    Article  Google Scholar 

  • Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791

    Article  Google Scholar 

  • Hajek AE, Delalibera JI (2010) Fungal pathogens as classical biological control agents against arthropods. BioControl 55:147–158. doi:10.1007/s10526-009-9253-6

    Article  Google Scholar 

  • Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98

    CAS  Google Scholar 

  • Hylis M, Weiser J, Oborník M, Vávra J (2005) DNA isolation from museum and type collection slides of microsporidia. J Invertebr Pathol 88:257–260. doi:10.1016/j.jip.2005.02.004

    Article  CAS  PubMed  Google Scholar 

  • Kimura M (1980) A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120

    Article  CAS  PubMed  Google Scholar 

  • Larsson JIR (2014) The primitive microsporidia. In: Microsporidia: pathogens of opportunity. First ed. (Eds: Weiss L.M. and Becnel J.J.). John Wiley & Sons, Inc., pp. 605–635

  • O’Mahony EM, Tay WT, Paxton RJ (2007) Multiple rRNA variants in a single spore of the microsporidian Nosema bombi. J Eukaryot Microbiol 54:103–109. doi:10.1111/j.1550-7408.2006.00232.x

    Article  PubMed  Google Scholar 

  • Reynolds ES (1963) The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol 17:208–212. doi:10.1083/jcb.17.1.208

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Solter LF, Becnel JJ, David HO (2012) Microsporidian Entomopathogens. In: Vega, F.E., Kaya, H.K. (Eds.), Insect Pathology. Elsevier Inc., pp. 221–263

  • Sprague V, Becnel JJ, Hazard EI (1992) Taxonomy of phylum Microspora. Crit Rev Microbiol 18:285–395

    Article  CAS  PubMed  Google Scholar 

  • Spurr AR (1969) A low-viscosity epoxy resin embedding medium for electron microscopy. Clin Microbiol Res 3:197–218

    Google Scholar 

  • Toguebaye BS, Bouix G (1989) Nosema galerucellae n. sp. Microsporidian (Protozoa, Microspora), parasite of Galerucella luteola Müller (Chrysomelidae, Coleoptera): development cycle and ultrastructure. Eur J Protistol 24:346–353. doi:10.1016/S0932-4739(89)80005-7

    Article  CAS  PubMed  Google Scholar 

  • Undeen AH, Vávra J (1997) Research methods for entomopathogenic protozoa. In: Lacey L (ed) Manual of techniques in insect pathology. Biological techniques series. Academic Press, London, pp 117–151

    Chapter  Google Scholar 

  • Vossbrinck CR, Debrunner-Vossbrinck BA (2005) Molecular phylogeny of the microsporidia: ecological, ultrastructural and taxonomic considerations. Folia Parasitol 52:131–142. doi:10.14411/fp.2005.017

    Article  CAS  PubMed  Google Scholar 

  • Vossbrinck CR, Maddox JV, Frideman S, Debrunner-Vossbrinck BA, Woese CR (1987) Ribosomal RNA sequence suggests microsporidia are extremely ancient eukaryotes. Nature 326:411–414. doi:10.1038/326411a0

    Article  CAS  PubMed  Google Scholar 

  • Vossbrinck CR, Baker MD, Didier ES, Debrunner-Vossbrinck BA, Shadduck JA (1993) Ribosomal DNA sequences of Encephalitozoon hellem and Encephalitozoon cuniculi: species identification and phylogenetic construction. J Eukaryot Micorbiol 40:354–362. doi:10.1111/j.1550-7408.1993.tb04928.x

    Article  CAS  Google Scholar 

  • Wang Y, Liu W, Jiang Y, Huang L, Irfan M, Shi S, Yang R, Qin L (2015) Morphological and molecular characterization of Nosema pernyi a microsporidian parasite in Antheraea pernyi. Parasitol Res 114:3327–3336. doi:10.1007/s00436-015-4558-0

    Article  PubMed  Google Scholar 

  • Weiser J, Wegensteiner R, Zizka Z (1995) Canningia spinidentis gen. et sp. n. (Protista: Microspora), a new pathogen of the fir bark beetle Pityokteines spinidens. Folia Parasitol 42:1–10

    Google Scholar 

  • Wu Z, Jamieson S, Kielbaso J (1991) Urban forest pest management. J Arbor 17:150–158

    Google Scholar 

  • Yaman M, Bekircan Ç, Radek R, Linde A (2014) Nosema pieriae sp. n. (Microsporida, Nosematidae): a new microsporidian pathogen of the Cabbage Butterfly Pieris brassicae L. (Lepidoptera: Pieridae). Acta Protozool 53:223–232. doi:10.4467/16890027AP.14.019.1600

    Google Scholar 

Download references

Acknowledgments

The TEM analysis of this study was carried out in Eskişehir Osmangazi University (ESOGU) Central Research Laboratory, Research and Application Centre (ARUM). We are thankful to Assoc. Prof. İlknur Dağ and her team for making this study possible and her valuable comments.

Author information

Authors and Affiliations

  1. Department of Biology, Faculty of Sciences, Karadeniz Technical University, 61080, Trabzon, Turkey

    Çağrı Bekircan & Ufuk Bülbül

  2. Department of Molecular Biology and Genetic, Faculty of Sciences, Karadeniz Technical University, 61080, Trabzon, Turkey

    Halil İ. Güler

  3. United States Department of Agriculture, Agriculture Research Service, Center for Medical, Agricultural and Veterinary Entomology (CMAVE), 1600 SW 23rd Drive, Gainesville, FL, USA

    James J. Becnel

Authors
  1. Çağrı Bekircan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ufuk Bülbül
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Halil İ. Güler
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. James J. Becnel
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Çağrı Bekircan.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Bekircan, Ç., Bülbül, U., Güler, H.İ. et al. Description and phylogeny of a new microsporidium from the elm leaf beetle, Xanthogaleruca luteola Muller, 1766 (Coleoptera: Chrysomelidae). Parasitol Res 116, 773–780 (2017). https://doi.org/10.1007/s00436-016-5349-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00436-016-5349-y

Keywords

Search

Navigation