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Henneguya paraensis n. sp. (Myxozoa; Myxosporea), a new gill parasite of the Amazonian fish Cichla temensis (Teleostei: Cichlidae): morphological and molecular aspects

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Abstract

The present study describes light microscopy, transmission electron microscopy, and molecular analyses of a myxosporid found parasitizing the gill region of the teleost fish Cichla temensis, collected from the Tocantins River, near Cametá, Pará State, Brazil. The prevalence of infection was 60 %. The spore-containing cysts that were located in the gill lamellae were oval and whitish. The spores had a mean length of 42.3 ± 0.65 μm; fusiform body, 12.8 ± 0.42-μm long and 8.6 ± 0.32-μm wide; each of the two valves exhibited a tapering tail of 29.5 ± 0.73 μm length. The spores had two polar capsules, 7.4 ± 0.16-μm long by 2.6 ± 0.08-μm wide, containing a polar filament with 5–7 twists. The spores differ from the species previously described, and phylogenetic analysis based on spore morphology and molecular aspects indicated that the fish parasite Henneguya sp. has a strong trend to form clades mainly based on the environment and host order/family. Thus, we conclude that the species belongs to the family Myxobolidae, genus Henneguya, which comprises a new species: Henneguya paraensis n. sp.

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Acknowledgments

We are grateful to Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Fundação Amazônia Paraense deAmparo à Pesquisa (FAPESPA), and the microscopy electronic laboratory the Instituto Evandro Chagas (IEC).

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Authors and Affiliations

  1. Carlos Azevedo Research Laboratory, Federal Rural University of the Amazon (UFRA), Avenida Presidente Tancredo Neves, 2501, Montese, Belém, 66077-901, Pará, Brazil

    Michele Velasco & Edilson Matos

  2. Laboratory of Morphophysiology and Animal Health, Amapá State University (UEAP), Macapá, Amapá, Brazil

    Marcela Videira

  3. Laboratory of Biomolecular Technology, Federal University of Pará (UFPA), Belém, Pará, Brazil

    Luciana de Cássia Silva do Nascimento & Evonnildo Costa Gonçalves

  4. Edilson Matos Research Laboratory (UFPA), Belém, Pará, Brazil

    Patrícia Matos

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  1. Michele Velasco
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  2. Marcela Videira
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  3. Luciana de Cássia Silva do Nascimento
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  4. Patrícia Matos
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  5. Evonnildo Costa Gonçalves
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  6. Edilson Matos
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Correspondence to Edilson Matos.

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Velasco, M., Videira, M., Nascimento, L.d.C.S.d. et al. Henneguya paraensis n. sp. (Myxozoa; Myxosporea), a new gill parasite of the Amazonian fish Cichla temensis (Teleostei: Cichlidae): morphological and molecular aspects. Parasitol Res 115, 1779–1787 (2016). https://doi.org/10.1007/s00436-016-4916-6

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