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Excretory system of representatives from family Diplozoidae (Monogenea)

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Abstract

Diplozoons are representatives of blood-feeding ectoparasites from the family Diplozoidae (Polyopisthocotylea, Monogenea). Although these worms have been the subject of numerous taxonomical, phylogenetic, and ecological studies, the detailed study of their excretory system has remained relatively neglected. Our observations focused on the morphological and ultrastructural features of the excretory apparatus of four diplozoid species: Diplozoon paradoxum, Eudiplozoon nipponicum, Paradiplozoon bliccae, and Paradiplozoon homoion. Observations were obtained using two microscope methods: light microscopy, equipped with differential interference contrast (Nomarski DIC) and transmission electron microscopy (TEM). The ultrastructure of two basic compartments which forms the excretory apparatus, flame cells with filtration apparatus, and canal cells forming the protonephridial ducts is revealed in this study. A unique consecutive sequence of longitudinal semi-thin sections of the excretory pore of E. nipponicum is visualized there for the first time.

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Acknowledgments

The authors would like to thank the Faculty of Science, Masaryk University Brno. A big thank you goes to Professor Iva Dyková for her support with TEM observations, helpful comments, and kind approach. The authors also thank the Laboratory of Electron Microscopy, Biology Centre of ASCR, České Budějovice, Czech Republic, and Petra Masařová for TEM sample preparation. Great thanks go to Gabriela Vágnerová and Eliška Šrámová for their help with fish autopsies and to Peter Daniel Stuart for English correction of this text. VK, BK, and MG were supported by the Czech Science Foundation, no. GAP506/12/1258. MK and JI were supported by the Czech Science Foundation, projects to promote excellence in basic research, no. GBP505/12/G112.

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Authors and Affiliations

  1. Department of Botany and Zoology, Faculty of Science, Masaryk University, Kotlářská 2, 611 37, Brno, Czech Republic

    V. Konstanzová, B. Koubková, M. Kašný, J. Ilgová & M. Gelnar

  2. Department of Parasitology, Faculty of Science, Charles University in Prague, Viničná 7, 128 44, Prague, Czech Republic

    M. Kašný

  3. Department of Medical Biology, Faculty of Medical Sciences, Warmia and Mazury University, Żolnierska 14c, 10-561, Olsztyn, Poland

    E. Dzika

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  1. V. Konstanzová
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  2. B. Koubková
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  3. M. Kašný
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  6. M. Gelnar
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Correspondence to V. Konstanzová.

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Konstanzová, V., Koubková, B., Kašný, M. et al. Excretory system of representatives from family Diplozoidae (Monogenea). Parasitol Res 115, 1493–1500 (2016). https://doi.org/10.1007/s00436-015-4882-4

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  • DOI: https://doi.org/10.1007/s00436-015-4882-4

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