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Sarcocystis oreamni, n. sp. (Apicomplexa: Sarcocystidae) from the mountain goat (Oreamnos americanus)

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An Erratum to this article was published on 29 October 2015

Abstract

Numerous species of Sarcocystis have been reported from wild ruminants, but none has been named from the Rocky Mountain goat (Oreamnos americanus). Mature sarcocysts were found in frozen muscle samples of three of seven mountain goats from Alaska, USA. Two morphological types of sarcocysts were found; one had Sarcocystis cornagliai-like sarcocysts, previously named from the Alpine ibex (Capra ibex) from Europe. Two other goats were infected with a new species, Sarcocystis oreamni. Sarcocystis oreamni sarcocysts were microscopic with 2 μm-thick sarcocyst wall. By transmission electron microscopy, the sarcocyst wall had 1.7 μm-thick with unusual molar tooth-like villar protrusions (vp), type 29. The vp had an electron dense core and two disc-shaped plaques at the tip with fine microtubules. Bradyzoites were 8.6–9.1 μm long. Single nucleotide polymorphism (SNP) identified in 18S rRNA, and 28S rRNA loci of rDNA regions that suggested S. oreamni molecularly apart from related species. The phylogenetic analysis based on 18S rRNA and 28S rRNA sequences suggested S. oreamni is related with Sarcocystis species that employ members of the Canidae family as their definitive host.

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Acknowledgements

The authors thank Mr. Efrain Pérez and Joseph Madary, Joint Pathology Center, Veterinary Services, U.S. Army, Silver Spring, Maryland for excellent technical help with electron microscopy. Jeff Jemison, Alaska Department of Fish and Game, Juneau, AK archived tissue samples. R. Calero-Bernal is a postdoctoral fellow (ref. PO12010) funded by the Department of Employment and Innovation of the Regional Government of Extremadura (Spain) and the European Social Fund.

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Authors and Affiliations

  1. United States Department of Agriculture, Agricultural Research Service, Beltsville Agricultural Research Center, Animal Parasitic Diseases Laboratory, Beltsville, MD, 20705-2350, USA

    Rafael Calero-Bernal, Shiv K. Verma, Camila K. Cerqueira-Cézar & Jitender P. Dubey

  2. Alaska Department of Fish and Game, Division of Wildlife Conservation, Juneau, AK, 99811, USA

    Erna Van Wilpe

  3. Department of Anatomy and Physiology, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, 0110, South Africa

    Kevin White

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  1. Rafael Calero-Bernal
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  2. Erna Van Wilpe
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  3. Kevin White
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  4. Shiv K. Verma
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  5. Camila K. Cerqueira-Cézar
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  6. Jitender P. Dubey
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Correspondence to Jitender P. Dubey.

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Calero-Bernal, R., Van Wilpe, E., White, K. et al. Sarcocystis oreamni, n. sp. (Apicomplexa: Sarcocystidae) from the mountain goat (Oreamnos americanus). Parasitol Res 114, 4135–4141 (2015). https://doi.org/10.1007/s00436-015-4644-3

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  • DOI: https://doi.org/10.1007/s00436-015-4644-3

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