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Application of polymerase chain reaction on cerebrospinal fluid for diagnosis of cerebral coenurosis in small ruminants

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Abstract

Sheep and goats serve as intermediate hosts for the canine tapeworm Taenia multiceps. The cysts produced by the intermediate stage of parasite are usually found in the cerebral hemispheres of small ruminants, and the resulting disease is commonly known as coenurosis. Coenurosis is clinically manifested in the form of various nervous symptoms, depending on the exact location of the cyst. The variety of neurological symptoms contributes to the complexity of clinical diagnosis and reinforces the need for a more specific and acceptable diagnostic approach. We demonstrated here, for the first time, that the T. multiceps DNA is present in the cerebrospinal fluid (CSF) of the infected sheep and goats. In addition, the molecular genetic marker of the mitochondrial DNA was applied phylogenetically to show that our isolates together with other T. multiceps strains comprised a monophyletic group that is a sister to Taenia krabbei. Pairwise comparison between the cox1 sequences of our study and other T. multiceps genotypes existing in the GenBank showed similarity ranging from 98 to 100 %. Accordingly, the polymerase chain reaction (PCR) can be used for amplification of DNA of the parasite originated from the CSF and provides a valuable method for accurate identification of coenurosis cases.

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Authors and Affiliations

  1. Department of Pathology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran

    Ahmad Oryan & Omidreza Amrabadi

  2. Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran

    Hassan Sharifiyazdi & Mohsen Ghane

  3. Department of Parasitology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran

    Mohammad Moazeni & Maryam Akbari

Authors
  1. Ahmad Oryan
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  2. Omidreza Amrabadi
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  3. Hassan Sharifiyazdi
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  4. Mohammad Moazeni
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  5. Maryam Akbari
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  6. Mohsen Ghane
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Correspondence to Ahmad Oryan.

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Oryan, A., Amrabadi, O., Sharifiyazdi, H. et al. Application of polymerase chain reaction on cerebrospinal fluid for diagnosis of cerebral coenurosis in small ruminants. Parasitol Res 114, 3741–3746 (2015). https://doi.org/10.1007/s00436-015-4603-z

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  • DOI: https://doi.org/10.1007/s00436-015-4603-z

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