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A specific indel marker for the Philippines Schistosoma japonicum revealed by analysis of mitochondrial genome sequences

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Abstract

In the present study, near-complete mitochondrial (mt) genome sequences for Schistosoma japonicum from different regions in the Philippines and Japan were amplified and sequenced. Comparisons among S. japonicum from the Philippines, Japan, and China revealed a geographically based length difference in mt genomes, but the mt genomic organization and gene arrangement were the same. Sequence differences among samples from the Philippines and all samples from the three endemic areas were 0.57–2.12 and 0.76–3.85 %, respectively. The most variable part of the mt genome was the non-coding region. In the coding portion of the genome, protein-coding genes varied more than rRNA genes and tRNAs. The near-complete mt genome sequences for Philippine specimens were identical in length (14,091 bp) which was 4 bp longer than those of S. japonicum samples from Japan and China. This indel provides a unique genetic marker for S. japonicum samples from the Philippines. Phylogenetic analyses based on the concatenated amino acids of 12 protein-coding genes showed that samples of S. japonicum clustered according to their geographical origins. The identified mitochondrial indel marker will be useful for tracing the source of S. japonicum infection in humans and animals in Southeast Asia.

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Acknowledgments

This work is supported, in part, by the Pearl River Science and Technology New Star Project (Grant No. 2014J2200096), the President’s Foundation of Guangdong Academy of Agricultural Sciences (Grant No. 201413), the Science Fund for Creative Research Groups of Gansu Province (Grant No. 1210RJIA006), and the “Special Fund for Agro-scientific Research in the Public Interest” (Grant No. 201303037).

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Authors and Affiliations

  1. Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, Guangdong Province, People’s Republic of China

    Juan Li

  2. State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, CAAS, Lanzhou, 730046, Gansu Province, People’s Republic of China

    Juan Li & Xing-Quan Zhu

  3. Department of Basic Medicine, Xiangnan University, Chenzhou, 423000, Hunan Province, People’s Republic of China

    Fen Chen

  4. Department of Parasitology, National Institute of Infectious Diseases, Tokyo, 113-8421, Japan

    Hiromu Sugiyama

  5. School of Marine and Tropical Biology, James Cook University, Townsville, Queensland, 4811, Australia

    David Blair

  6. College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, Guangdong Province, People’s Republic of China

    Rui-Qing Lin

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  1. Juan Li
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  2. Fen Chen
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  3. Hiromu Sugiyama
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Correspondence to Xing-Quan Zhu.

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Li, J., Chen, F., Sugiyama, H. et al. A specific indel marker for the Philippines Schistosoma japonicum revealed by analysis of mitochondrial genome sequences. Parasitol Res 114, 2697–2704 (2015). https://doi.org/10.1007/s00436-015-4475-2

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  • DOI: https://doi.org/10.1007/s00436-015-4475-2

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