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Glucosinolate from leaf of Solanum nigrum L. (Solanaceae) as a new mosquito larvicide

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Abstract

The present study was carried out to investigate the biocontrol potentiality of active ingredient isolated from ethyl acetate extract of mature leaves of Solanum nigrum L. (Solanaceae) against the larval form of Culex quinquefasciatus Say. Mortality rate at a concentration of 25 mg/L of the active compound was highest (P < 0.05) amongst all tested concentrations. Result of log-probit analysis (at 95 % confidence level) revealed that LC50 and LC90 values are inversely proportional to exposure period of bioassay. A clear dose-dependent mortality was observed, as the rate of mortality (Y) was positively correlated with the concentrations of the compound (X); having regression coefficient value close to 1. The compound was found to be ecofriendly as it did not show any adverse effect to the studied nontarget organisms. Chemical characterization of the active ingredient was also carried out by infrared spectroscopic analysis (IR), mass analyses (GC-MS) and carbon-hydrogen-nitrogen-sulphur analyses (CHNS), that revealed the presence of a glucosinolate compound [1-thio-ß-d-glucopyranose-1-[(R)-3-hydroxy-2-ethyl-N-hydroxysulfonyloxy propanimidate] having the molecular formula of C11H21NO10S2.

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Acknowledgments

The authors would like to acknowledge/thank the help of Dr. Ambarish Mukherjee, professor of botany in the University of Burdwan, for the identification of the plant. The financial support provided by the DST, New Delhi, India (D.O. NO.SR/SO/HS/84/2007 dated 08/02/08) is thankfully acknowledged. The financial support provided by the University Grants Commission [PSW 001/11-12(ERO-3811)] to Dr. Anupam Ghosh is also thankfully acknowledged.

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There is no conflict of interest.

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Authors and Affiliations

  1. Department of Zoology Mosquito and Microbiology Research Units, University of Burdwan, Burdwan, West Bengal, 713104, India

    Anjali Rawani & Goutam Chandra

  2. Department of Zoology, Bankura Christian College, Bankura, West Bengal, 722101, India

    Anupam Ghosh

  3. Department of Chemistry Natural Product Laboratory, University of Burdwan, Burdwan, West Bengal, India

    Subrata Laskar

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  1. Anjali Rawani
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  2. Anupam Ghosh
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  3. Subrata Laskar
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  4. Goutam Chandra
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Correspondence to Goutam Chandra.

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Rawani, A., Ghosh, A., Laskar, S. et al. Glucosinolate from leaf of Solanum nigrum L. (Solanaceae) as a new mosquito larvicide. Parasitol Res 113, 4423–4430 (2014). https://doi.org/10.1007/s00436-014-4120-5

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  • DOI: https://doi.org/10.1007/s00436-014-4120-5

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