Abstract
Two new species of Cichlidogyrus are described from the gills of three species of African cichlids collected from the Gambia River basin in the Niokolo-Koba National Park, Senegal: Cichlidogyrus dracolemma n. sp. from Hemichromis letourneuxi and Cichlidogyrus nageus n. sp. from Sarotherodon galilaeus and Tilapia guineensis. The new species are recognized on the basis of morphological differences in the male copulatory organs (MCOs) and apparent differences in the SSU rDNA and ITS1 sequence data. C. dracolemma n. sp. resembles specimens of Cichlidogyrus from Hemichromis bimaculatus, probably misidentified as Cichlidogyrus bychowskii by Paperna in 1965, in having morphologically similar types of haptoral sclerites and MCO. The new species is characterized by possessing an MCO with a kite-shaped termination of the accessory piece and a vagina distally surrounded by a circular superficial sclerotization of the body surface. Molecular phylogenetic analyses showed a strongly supported clade including C. dracolemma n. sp. and two species collected from Hemichromis fasciatus, namely Cichlidogyrus longicirrus Paperna, 1965 and Cichlidogyrus falcifer Dossou and Birgi, 1984. Based on both morphology and molecular phylogeny, C. nageus n. sp. appears to be closely related to Cichlidogyrus acerbus Dossou, 1982 obtained from S. galilaeus. Morphologically, C. nageus n. sp. is readily separated from all known congeners by the characteristic pestle shaped termination of the accessory piece of the MCO. Molecular phylogenetic reconstruction showed that C. dracolemma n. sp. and C. nageus n. sp. cluster with the Cichlidogyrus species possessing the same haptoral configuration and host preferences (on subfamily and tribe level, respectively).
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References
Beverley-Burton M, Klassen GJ (1990) New approaches to the systematics of the ancyrocephalid Monogenea from Nearctic freshwater fishes. J Parasitol 76:1–12
Birgi E, Euzet L (1983) Monogènes parasited des poissons des eaux douces du Cameroun Présence des genre Cichlidogyrus et Dactylogyrus chez Aphyosemion (Cyprinodontidae). Bull Soc Zool Fr 108:101–106
Bukinga FM, Vanhove MPM, Van Steenberge M, Pariselle A (2012) Ancyrocephalidae (Monogenea of Lake Tanganyika: III: Cichlidogyrus infecting the world’s biggest cichlid and the non-endemic tribes Haplochromini, Oreochromini and Tylochromini (Teleostei, Cichlidae). Parasitol Res 111:2049–2061. doi:10.1007/s00436-012-3052-1
Domingues MV, Boeger WA (2008) Phylogeny and revision of Diplectanidae Monticelli, 1903 (Platyhelminthes: Monogenoidea). Zootaxa 1698:1–40
Dossou C (1982) Parasites de poissons d’eau douce du Bénin III. Espèces nouvelles du genre Cichlidogyrus (Monogenea) parasites de Cichlidae. Bulletin de l’IFAN 44:295–322
Douëllou L (1993) Monogeneans of the genus Cichlidogyrus Paperna, 1960 (Dactylogyridae: Ancyrocephalinae) from cichlid fishes of Lake Kariba (Zimbabwe) with descriptions of five new species. Syst Parasitol 25:159–186
Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791
Gillardin C, Vanhove MPM, Pariselle A, Huyse T, Volckaert FAM (2012) Ancyrocephalidae (Monogenea) of Lake Tanganyika: II: description of the first Cichlidogyrus spp. parasites from Tropheini fish hosts (Teleostei, Cichlidae). Parasitol Res 110:305–313. doi:10.1007/s00436-011-2490-5
Gussev AV (1962) Monogenoidea. In: Bychovskaya-Pavlovskaya IE et al (eds) Key to parasites of freshwater fish of the USSR. Moscow-Leningrad: Academiya Nauk SSSR [Translated from Russian by Israel Program Scientgic Translation, Ser. No. 1136, Jerusalem, 1964)]
Gussev AV (1983) The method of collecting and processing of fish parasitic monogenean material. Nauka, Leningrad, 48 p. [In Russian]
Hall TA (1999) Bioedit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98
Kritsky DC, Boeger WA (2002) Neotropical Monogenoidea. 41: New and previously described species of Dactylogyridae (Platyhelminthes) from the gills of marine and freshwater perciform fishes (Teleostei) with proposal of a new genus and a hypothesis on phylogeny. Zoosystema 24:7–40
Kritsky DC, Thatcher VE, Boeger WA (1986) Neotropical monogenea. 8. Revision of Urocleidoides (Dactylogyridae, Ancyrocephalinae). P Helm Soc Wash 53:1–37
Markevich AP (1934) Parasitic diseases of fish and their control. Koiz, Leningrad [in Russian]
Mendlová M, Pariselle A, Vyskočilová M, Šimková A (2010) Molecular phylogeny of monogeneans parasitizing African freshwater fish of Cichlidae inferred from LSU rDNA sequences. Parasitol Res 107:1405–1413. doi:10.1007/s00436-010-2008-6
Mendlová M, Desdevises Y, Civáňová K, Pariselle A, Šimková A (2012) Monogeneans of West African cichlid fish: evolution and cophylogenetic interaction. PLoS One 7:e37268. doi:10.1371/journal.pone.0037268
Mizelle JD (1936) New species of trematodes from the gills of Illinois fishes. Am Midl Nat 17:785–806
Musilová N, Řehulková E, Gelnar M (2009) Dactylogyrids (Platyhelminthes: Monogenea) from the gills of the African carp, Labeo coubie Rüppell (Cyprinidae), from Senegal, with descriptions of three new species of Dactylogyrus and the redescription of Dactylogyrus cyclocirrus Paperna, 1973. Zootaxa 2241:47–68
N’Douba V, Thys van den Audenaerde DFE, Pariselle A (1997) Description d’une espèce nouvelle de Monogène ectoparasite branchial de Tilapia guineensis (Bleeker, 1862) (Cichlidae) en Côte d’Ivoire. J Afr Zool 111:429–433
Paperna I (1960) Studies on monogenetic trematodes in Israel. 2. Monogenetic trematodes of cichlids. Bamidgeh. Bull Fish Cult Israel 12:20–33
Paperna I (1964) Parasitic helminths of inland-water fishes in Israel. Israel J Zool 13:1–26
Paperna I (1965) Monogenetic trematodes collected from fresh water fish in southern Ghana. Bamidgeh Bull Fish Cult Israel 17:107–115
Paperna I (1968) Monogenetic trematodes collected from fresh water fish in Ghana. Second report. Bamidgeh. Bull Fish Cult Israel 20:88–99
Paperna I (1969) Monogenetic trematodes of the fish of the Volta basin and South Ghana. Bulletin de l’IFAN 31:840–880
Paperna I (1979) Monogenea of inland water fish in Africa. Ann Musee Roy Afr Centr Ser 8 Sci Zool 226:1–131
Paperna I, Thurston JP (1969) Monogenetic trematodes collected from cichlid fish in Uganda; including the description of five new species of Cichlidogyrus. Rev Zool Bot Afr 79:15–33
Pariselle A, Euzet L (1995) Gill parasites of the genus Cichlidogyrus Paperna, 1960 (Monogenea, Ancyrocephalidae) from Tilapia guineensis (Bleeker, 1862), with descriptions of six new species. Syst Parasitol 30:187–198
Pariselle A, Euzet L (1996) Cichlidogyrus Paperna, 1960 (Monogenea, Ancyrocephalidae): gill parasites from West African Cichlidae of the subgenus Coptodon Regan, 1920 (Pisces), with descriptions of six new species. Syst Parasitol 34:109–124
Pariselle A, Euzet L (2003) Four new species of Cichlidogyrus (Monogenea: Ancyrocephalidae), gill parasites of Tilapia cabrae (Teleostei: Cichlidae), with discussion on relative length of haptoral sclerites. Folia Parasit 50:195–201
Pariselle A, Euzet L (2009) Systematic revision of dactylogyridean parasites (Monogenea) from cichlid fishes in Africa, the Levant and Madagascar. Zoosystema 31:849–898
Pariselle A, Bilong Bilong CF, Euzet L (2003) Four new species of Cichlidogyrus Paperna, 1960 (Monogenea, Ancyrocephalidae) all gill parasites from African mouthbreeder tilapias of the genera Sarotherodon and Oreochromis (Pisces, Cichlidae), with a re-description of C. thurstonae Ergens, 1981. Syst Parasitol 56:201–210
Posada D, Crandal AK (1998) Modeltest: testing the model of DNA substitution. Bioinformatics 14:817–818
Pouyaud L, Desmarais E, Deveney M, Pariselle A (2006) Phylogenetic relationships among monogenean gill parasites (Dactylogyridae, Ancyrocephalidae) infesting tilapiine hosts (Cichlidae): Systematic and evolutionary implications. Mol Phylogenet Evol 38:241–249. doi:10.1016/j.ympev.2005.08.013
Šimková A, Plaisance L, Matějusová I, Morand S, Verneau O (2003) Phylogenetic relationships of the Dactylogyridae Bychowsky, 1933 (Monogenea: Dactylogyridea): the need for the systematic revision of the Ancyrocephalinae Bychowsky, 1937. Syst Parasitol 54:1–11. doi:10.1017/S0031182006009942
Šimková A, Verneau O, Gelnar M, Morand S (2006) Specificity and specialization of congeneric monogeneans parasitizing cyprinid fish. Evolution 60:1023–1037
Swofford DL (2003) PAUP* Phylogenetic analysis using parsimony (*and other methods). Version 4.0b10. Sunderland. Sinauer Associates, Inc, Sunderland
Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTALW: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680
Vanhove MPM, Volckaert FAM, Pariselle A (2011) Ancyrocephalidae (Monogenea) of Lake Tanganyika: I: Four new species of Cichlidogyrus from Ophthalmotilapia ventralis (Teleostei, Cichlidae), the first record of this family in the basin. Zoologia 28:253–263. doi:10.1590/S1984-46702011000200016
Vignon M, Pariselle A, Vanhove MPM (2011) Modularity in attachment organs of African Cichlidogyrus (Platyhelminthes, Monogenea, Ancyrocephalidae) reflects phylogeny rather than host specificity or geographic distribution. Biol J Linn Soc 102:694–706
Whittington ID, Chisholm LA (2008) Diseases caused by Monogenea. In: Eiras J, Segner H, Wahli T, Kapoor BG (eds) Fish diseases. Science Publishers, Enfield, pp 683–816
Acknowledgments
Thanks are due to Radim Blažek, Naďa Musilová and Martina Dávidová (Masaryk University, Czech Republic) for their participation in collecting the parasites. We are also indebted to Martin Reichard and Barbora Bímová (Academy of Sciences of the Czech Republic) for fish collection and identification. This study (including all preparation and analyses) was supported by the Czech Science Foundation (project no. P505/12/G112).
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Řehulková, E., Mendlová, M. & Šimková, A. Two new species of Cichlidogyrus (Monogenea: Dactylogyridae) parasitizing the gills of African cichlid fishes (Perciformes) from Senegal: morphometric and molecular characterization. Parasitol Res 112, 1399–1410 (2013). https://doi.org/10.1007/s00436-013-3291-9
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DOI: https://doi.org/10.1007/s00436-013-3291-9