Parasitology Research

, Volume 112, Issue 2, pp 751–759

The microsporidian parasites Nosema ceranae and Nosema apis are widespread in honeybee (Apis mellifera) colonies across Scotland

Authors

  • Karen A Bollan
    • Division of Neuroscience, Medical Research Institute, Ninewells Medical SchoolUniversity of Dundee
  • J. Daniel Hothersall
    • Division of Neuroscience, Medical Research Institute, Ninewells Medical SchoolUniversity of Dundee
  • Christopher Moffat
    • Division of Neuroscience, Medical Research Institute, Ninewells Medical SchoolUniversity of Dundee
  • John Durkacz
    • Division of Neuroscience, Medical Research Institute, Ninewells Medical SchoolUniversity of Dundee
  • Nastja Saranzewa
    • Division of Neuroscience, Medical Research Institute, Ninewells Medical SchoolUniversity of Dundee
  • Geraldine A. Wright
    • Institute of Neuroscience, School of Biology, Ridley BuildingNewcastle University
  • Nigel E. Raine
    • School of Biological Sciences, Royal HollowayUniversity of London
  • Fiona Highet
    • Science and Advice for Scottish Agriculture
    • Division of Neuroscience, Medical Research Institute, Ninewells Medical SchoolUniversity of Dundee
Original Paper

DOI: 10.1007/s00436-012-3195-0

Cite this article as:
Bollan, K.A., Hothersall, J.D., Moffat, C. et al. Parasitol Res (2013) 112: 751. doi:10.1007/s00436-012-3195-0

Abstract

Nosema ceranae is spreading into areas where Nosema apis already exists. N. ceranae has been reported to cause an asymptomatic infection that may lead, ultimately, to colony collapse. It is thought that there may be a temperature barrier to its infiltration into countries in colder climates. In this study, 71 colonies from Scottish Beekeeper’s Association members have been screened for the presence of N. apis and N. ceranae across Scotland. We find that only 11 of the 71 colonies tested positive for spores by microscopy. However, 70.4 % of colonies screened by PCR revealed the presence of both N. ceranae and N. apis, with only 4.2 or 7 % having either strain alone and 18.3 % being Nosema free. A range of geographically separated colonies testing positive for N. ceranae were sequenced to confirm their identity. All nine sequences confirmed the presence of N. ceranae and indicated the presence of a single new variant. Furthermore, two of the spore-containing colonies had only N. ceranae present, and these exhibited the presence of smaller spores that could be distinguished from N. apis by the analysis of average spore size. Differential quantification of the PCR product revealed N. ceranae to be the dominant species in all seven samples tested. In conclusion, N. ceranae is widespread in Scotland where it exists in combination with the endemic N. apis. A single variant, identical to that found in France (DQ374655) except for the addition of a single nucleotide polymorphism, is present in Scotland.

Supplementary material

436_2012_3195_MOESM1_ESM.doc (340 kb)
Supplementary Figure 1Size distribution of Nosema spores identified in Scotland using larger dataset. a Samples were distributed into two groups [group A (221 spores); samples 31, 34 and 66] and [group B (171 spores); samples15, 17, 18, and 42] based on size differences determined by ANOVA (Kruskal–Wallis; Dunnett’s post hoc test) and analyzed for length, width and square area. Data were analyzed using a Mann–Whitney test (*P < 0.001). b The scatter of the length, width and square area of the spores in each sample were plotted to illustrate their size distribution. Red lines represent the average of each dataset. (DOC 339 kb). (DOC 339 kb)

Copyright information

© Springer-Verlag Berlin Heidelberg 2012