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Multilocus genetic analysis of Cryptosporidium parvum from Egypt

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Abstract

Cryptosporidium parvum is a ubiquitous zonootic parasite causing enteritis in man and animals. Cryptosporidium infection was confirmed microscopically in neonatal calves (less than 6 weeks of age) at Kafr El Sheikh Province, Egypt. Multilocus analysis using a wide array of genetic markers was carried out to assess genetic diversity of C. parvum isolates. PCR amplification and partial sequence analysis of 70 kDa heat shock protein, dihydrofolate reductase, alpha-tubulin, elongation factor 1 alpha as well as thrombospondin-related anonymous protein of Cryptosporidium-1, and thrombospondin-related anonymous protein of Cryptosporidium-2 gene markers were achieved. Data indicated that the analyzed isolates belong to C. parvum genotype II with obvious sequence heterogeneity compared with counterparts deposited in Genebank.

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Authors and Affiliations

  1. Laboratory of Sustainable Environmental Biology,Field Centre studies, Graduate School of Agricultural Science, Tohoku University, 232-3 Yomogida, Naruko-onsen, Osaki, Miyagi, 989-6711, Japan

    Said Amer, Hajime Honma, Yasuhiro Fukuda, Chica Tada & Yutaka Nakai

  2. Department of Zoology, Faculty of Science, Kafr El Sheikh University, Kafr El Sheikh, 33516, Egypt

    Said Amer

  3. Department of Anatomy, Faculty of Veterinary Medicine, Kafr El Sheikh University, Kafr El Sheikh, 33516, Egypt

    Masoud Fayed

Authors
  1. Said Amer
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  2. Masoud Fayed
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  3. Hajime Honma
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  4. Yasuhiro Fukuda
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  5. Chica Tada
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  6. Yutaka Nakai
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Correspondence to Yutaka Nakai.

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Amer, S., Fayed, M., Honma, H. et al. Multilocus genetic analysis of Cryptosporidium parvum from Egypt. Parasitol Res 107, 1043–1047 (2010). https://doi.org/10.1007/s00436-010-1967-y

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  • DOI: https://doi.org/10.1007/s00436-010-1967-y

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