Abstract
Echinostoma caproni metacercarial cysts often need to be stored for several months to a year to meet laboratory requirements. This study examined the bacteria associated with encysted metacercariae stored at 4°C. Bacterial assemblages from the kidney-pericardial region of uninfected snails and assemblages from the site of metacercarial infection in Biomphalaria glabrata were determined. Four species of Gram-negative rods were isolated and identified from the stored cysts to species or genus as Pseudomonas sp., Alcaligenes sp., Enterobacter cloacae, and Aeromonas hydrophila. Gram-positive rods were also isolated and included small slender rods (alpha hemolytic), small coccobacilli (gamma hemolytic), and small rods that exhibited gamma hemolysis. Accumulation of these bacteria on the surface of encysted metacercariae probably contributed to a decreased cyst viability of E. caproni.
References
Aho JM, Uglem GL, Moore JP, Larson OR (1991) Bacteria associated with the tegument of Clinostomum marginatum (Digenea). J Parasitol 77:784–786
Cheng TC (1986) Biological control studies: bacteria associated with moribund Biomphalaria glabrata (Mollusca) in the laboratory. J Invertebr Pathol 47:219–224
Fried B, Huffman JE (1996) The biology of the intestinal trematode Echinostoma caproni. Adv Parasitol 38:311–368
Irwin SWB, Fried B (1990) Scanning and transmission electron microscopic observations metacercariae of Echinostoma trivolvis and Echinostoma caproni during in vitro excystation. J Helminthol Soc Wash 57:79–83
Li S, Kang HW, Choi MH, Hong ST (2006) Long-term storage of Clonorchis sinensis metacercariae in vitro. Parasitol Res 100:25–29
Machtiger NA, O’Leary WM (1971) Nutritional requirements of Arizona, Citrobacter, and Providencia. J Bacteriol 108:948–950
Nollen PM, Nadakavukaren K (1974) Megalodiscus temperatus: Scanning electron microscopy of the tegumental surfaces. Exp Parasitol 36:123–130
Rossi ML, Balfour CD, Fried B (2001) Excystation and infectivity of Echinostoma caproni metacercariae after prolonged storage. Comp Parasitol 68:126–129
Ursone RL, Fried B (1995) Light microscopic observations of Echinostoma caproni metacercariae during in vitro excystation. J Helminthol 69:253–257
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Keeler, S.P., Fried, B. & Huffman, J.E. Association of bacteria with metacercarial cysts of Echinostoma caproni . Parasitol Res 101, 1131–1133 (2007). https://doi.org/10.1007/s00436-007-0557-0
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DOI: https://doi.org/10.1007/s00436-007-0557-0