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Ultrastructural studies on the development of Enteromyxum scophthalmi (Myxozoa), an enteric parasite of turbot (Scophthalmus maximus L.)

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Abstract

The ultrastructure of the developmental stages of Enteromyxum scophthalmi is described. Scarce intracellular, early uninucleated stages appeared within intestinal epithelial cells whereas proliferative stages were abundant both intraepithelially and in the intestinal lumen. In the proliferative stages, food reserves were abundant in the cytoplasm of P cells and consisted mostly of carbohydrates in the intraepithelial stages and lipid inclusions in the luminal stages. Sporogenesis could occur in enveloped cells or by direct division or clustering of generative cells. The abundance, shape and size of mitochondria as well as the number and shape of their cristae were very variable in the different developmental stages. The cristae were usually tubular and sometimes plate-like, discoidal or lamellar. True flat cristae were not observed. We found elements of closed (cryptomitosis) and open mitosis as well as structures reminiscent of microtubule organising centres, hitherto not described in myxosporeans. The significance of these findings is discussed in relation to the taxonomic and phylogenetic position of the Myxozoa.

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Acknowledgements

This study was funded by the European Union and the Spanish Government through the research grant FEDER 1FD97-0679-C02-01. Additional support was provided by Stolt Sea Farm S.A. We are grateful to the Technical Services at the Universities of Valencia and Barcelona, and to M. del Carmen Carreira Valle, School of Veterinary Medicine of Lugo (University of Santiago), for assistance in the processing of electron microscopy samples.

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Authors and Affiliations

  1. Instituto de Acuicultura "Torre la Sal" (CSIC), Ribera de Cabanes, 12595, Castellón, Spain

    María J. Redondo, Oswaldo Palenzuela & Pilar Alvarez-Pellitero

  2. Departamento de Anatomía Patológica, Facultad de Veterinaria, Universidad de Santiago, Campus Universitario, 27002 , Lugo, Spain

    María I. Quiroga & José M. Nieto

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  1. María J. Redondo
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  2. María I. Quiroga
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  5. Pilar Alvarez-Pellitero
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Correspondence to Pilar Alvarez-Pellitero.

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Redondo, M.J., Quiroga, M.I., Palenzuela, O. et al. Ultrastructural studies on the development of Enteromyxum scophthalmi (Myxozoa), an enteric parasite of turbot (Scophthalmus maximus L.). Parasitol Res 90, 192–202 (2003). https://doi.org/10.1007/s00436-002-0810-5

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