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Expression of miRNAs in adenoid cystic carcinomas of the breast and salivary glands

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Abstract

Despite their similar histomorphologic appearance, adenoid cystic carcinomas of the breast and salivary glands (bACCs and sACCs, respectively) are clinically and pathologically diverse. We studied the expression levels of 18 microRNAs (miRNAs) in bACCs and sACCs and control normal breast and salivary gland tissues (bNs and sNs, respectively) by quantitative real-time polymerase chain reaction on formalin-fixed paraffin embedded tissues. miRNAs showing significant differences between the study groups were selected for target prediction. Increased expression of miR-17 and miR-20a was found in bACCs compared with bNs (p miR-17 = 0.017 and p miR-20a = 0.024, respectively), while the expression level of let-7b and miR-193b was lower in sACCs compared with normal sNs (p let-7b = 0.032 and p miR-193b = 0.023, respectively). Expression of miR-23b and miR-27b differed between normal breast and normal salivary gland tissue (p miR-23b = 0.007 and p miR-27b = 0.024, respectively), but not between bACCs and sACCs. The potential target mRNAs CCND1 and BCL2 were identified as reported targets of let-7b, miR-193b, miR-17, and miR-20a. Expression of their corresponding proteins cyclin D1 and Bcl-2 was studied by immunohistochemistry. We found both proteins overexpressed in bACCs as well as sACCs in comparison with corresponding normal tissues. However, expression of cyclin D1 and Bcl-2 proteins was not significantly different between bACCs and sACCs or between bNs and sNs. Although no differences in miRNA levels were found between bACCs and sACCs, in both organs, miRNA expression level was highly different between tumor tissue and control tissue.

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Acknowledgments

The authors thank Anna Szilágyi, Norbert Lippai, and Márta Jäckel for sending bACC cases for consultation purposes, Erzsebet Azumah for performing immunohistochemical assays, Krisztina Schlachter and Miklós Gergely Oláh for Adobe Photoshop management, Benedek Gyöngyösi for the qRT-PCR evaluation, and Barnabás Tóth for the statistical analysis.

Support grants: TÁMOP-4.2.2/B-10/1-2010-0013; TÁMOP-4.2.1/B-09/1/KMR-2010-0001; TÁMOP 4.2.4.A/2-11-1-2012-0001. Ethical approval: TUKEB 101/2012

Conflict of interest

The authors declare that they have no competing interests.

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Authors and Affiliations

  1. 2nd Department of Pathology, Semmelweis University, Budapest, Hungary

    Orsolya Kiss, A. Marcell Szász & Janina Kulka

  2. MTA-SE, Tumor Progression Research Group, 2nd Department of Pathology, Semmelweis University, Budapest, Hungary

    Anna-Mária Tőkés

  3. Department of Pathology, Clinical Center of the University of Sarajevo, Sarajevo, Bosnia Herzegovina

    Semir Vranic

  4. Caris Life Sciences, Phoenix, AZ, USA

    Zoran Gatalica

  5. Flór Ferenc Hospital, Kistarcsa, Hungary

    László Vass

  6. National Institute of Oncology, Budapest, Hungary

    Nóra Udvarhelyi

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  1. Orsolya Kiss
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Supplementary 1

Calculated ΔCT values of 18 microRNAs in the investigated study groups (sACCs: salivary-gland-derived adenoid cystic carcinomas; sNs: normal salivary gland tissues; bACCs: breast-derived adenoid cystic carcinomas; bNs: normal breast tissues) (XLS 44 kb)

Supplementary 2

Results of Tukey test performed on ΔCT values of microRNA expression of the investigated study groups (sACCs: salivary-gland-derived adenoid cystic carcinomas; sNs: normal salivary gland tissues; bACCs: breast-derived adenoid cystic carcinomas; bNs: normal breast tissues) (XLSX 30 kb)

Supplementary 3

Hirsch-score values of cyclin D1 and Bcl-2 immunohistochemical analysis (bN: normal breast tissue; bACC: breast-derived adenoid cystic carcinoma; sN: normal salivary gland tissue; sACC: salivary-gland-derived adenoid cystic carcinoma) (DOCX 12 kb)

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Kiss, O., Tőkés, AM., Vranic, S. et al. Expression of miRNAs in adenoid cystic carcinomas of the breast and salivary glands. Virchows Arch 467, 551–562 (2015). https://doi.org/10.1007/s00428-015-1827-3

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