Development Genes and Evolution

, Volume 206, Issue 4, pp 260–276

Mutations affecting pigmentation and shape of the adult zebrafish

Authors

  • P. Haffter
    • Max-Planck-Institut für Entwicklungsbiologie, Abteilung Genetik, Spemannstrasse 35, D-72076 Tübingen, Germany
  • Jörg Odenthal
    • Max-Planck-Institut für Entwicklungsbiologie, Abteilung Genetik, Spemannstrasse 35, D-72076 Tübingen, Germany
  • M. C. Mullins
    • Max-Planck-Institut für Entwicklungsbiologie, Abteilung Genetik, Spemannstrasse 35, D-72076 Tübingen, Germany
  • Shuo Lin
    • Massachusetts Institute of Technology, Center for Cancer Research, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA
  • Michael J. Farrell
    • Massachusetts Institute of Technology, Center for Cancer Research, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA
  • E. Vogelsang
    • Max-Planck-Institut für Entwicklungsbiologie, Abteilung Genetik, Spemannstrasse 35, D-72076 Tübingen, Germany
  • F. Haas
    • Max-Planck-Institut für Entwicklungsbiologie, Abteilung Genetik, Spemannstrasse 35, D-72076 Tübingen, Germany
  • M. Brand
    • Max-Planck-Institut für Entwicklungsbiologie, Abteilung Genetik, Spemannstrasse 35, D-72076 Tübingen, Germany
  • Fredericus J. M. van Eeden
    • Max-Planck-Institut für Entwicklungsbiologie, Abteilung Genetik, Spemannstrasse 35, D-72076 Tübingen, Germany
  • Makoto Furutani-Seiki
    • Max-Planck-Institut für Entwicklungsbiologie, Abteilung Genetik, Spemannstrasse 35, D-72076 Tübingen, Germany
  • Michael Granato
    • Max-Planck-Institut für Entwicklungsbiologie, Abteilung Genetik, Spemannstrasse 35, D-72076 Tübingen, Germany
  • M. Hammerschmidt
    • Max-Planck-Institut für Entwicklungsbiologie, Abteilung Genetik, Spemannstrasse 35, D-72076 Tübingen, Germany
  • Carl-Philipp Heisenberg
    • Max-Planck-Institut für Entwicklungsbiologie, Abteilung Genetik, Spemannstrasse 35, D-72076 Tübingen, Germany
  • Yun-Jin Jiang
    • Max-Planck-Institut für Entwicklungsbiologie, Abteilung Genetik, Spemannstrasse 35, D-72076 Tübingen, Germany
  • D. A. Kane
    • Max-Planck-Institut für Entwicklungsbiologie, Abteilung Genetik, Spemannstrasse 35, D-72076 Tübingen, Germany
  • R. N. Kelsh
    • Max-Planck-Institut für Entwicklungsbiologie, Abteilung Genetik, Spemannstrasse 35, D-72076 Tübingen, Germany
  • Nancy Hopkins
    • Massachusetts Institute of Technology, Center for Cancer Research, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA
  • Christiane Nüsslein-Volhard
    • Max-Planck-Institut für Entwicklungsbiologie, Abteilung Genetik, Spemannstrasse 35, D-72076 Tübingen, Germany
ORIGINAL ARTICLE

DOI: 10.1007/s004270050051

Cite this article as:
Haffter, P., Odenthal, J., Mullins, M. et al. Dev Gene Evol (1996) 206: 260. doi:10.1007/s004270050051

Abstract

 Mutations causing a visible phenotype in the adult serve as valuable visible genetic markers in multicellular genetic model organisms such as Drosophila melanogaster, Caenorhabditis elegans and Arabidopsis thaliana. In a large scale screen for mutations affecting early development of the zebrafish, we identified a number of mutations that are homozygous viable or semiviable. Here we describe viable mutations which produce visible phenotypes in the adult fish. These predominantly affect the fins and pigmentation, but also the eyes and body length of the adult. A number of dominant mutations caused visible phenotypes in the adult fish. Mutations in three genes, long fin, another long fin and wanda affected fin formation in the adult. Four mutations were found to cause a dominant reduction of the overall body length in the adult. The adult pigment pattern was found to be changed by dominant mutations in wanda, asterix, obelix, leopard, salz and pfeffer. Among the recessive mutations producing visible phenotypes in the homozygous adult, a group of mutations that failed to produce melanin was assayed for tyrosinase activity. Mutations in sandy produced embryos that failed to express tyrosinase activity. These are potentially useful for using tyrosinase as a marker for the generation of transgenic lines of zebrafish.

Key words Pigment patternFinFish skeletonTyrosinaseZebrafish

Copyright information

© Springer-Verlag Berlin Heidelberg 1996