ORIGINAL ARTICLE

Development Genes and Evolution

, Volume 206, Issue 4, pp 260-276

Mutations affecting pigmentation and shape of the adult zebrafish

  • P. HaffterAffiliated withMax-Planck-Institut für Entwicklungsbiologie, Abteilung Genetik, Spemannstrasse 35, D-72076 Tübingen, Germany
  • , Jörg OdenthalAffiliated withMax-Planck-Institut für Entwicklungsbiologie, Abteilung Genetik, Spemannstrasse 35, D-72076 Tübingen, Germany
  • , M. C. MullinsAffiliated withMax-Planck-Institut für Entwicklungsbiologie, Abteilung Genetik, Spemannstrasse 35, D-72076 Tübingen, Germany
  • , Shuo LinAffiliated withMassachusetts Institute of Technology, Center for Cancer Research, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA
  • , Michael J. FarrellAffiliated withMassachusetts Institute of Technology, Center for Cancer Research, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA
  • , E. VogelsangAffiliated withMax-Planck-Institut für Entwicklungsbiologie, Abteilung Genetik, Spemannstrasse 35, D-72076 Tübingen, Germany
  • , F. HaasAffiliated withMax-Planck-Institut für Entwicklungsbiologie, Abteilung Genetik, Spemannstrasse 35, D-72076 Tübingen, Germany
  • , M. BrandAffiliated withMax-Planck-Institut für Entwicklungsbiologie, Abteilung Genetik, Spemannstrasse 35, D-72076 Tübingen, Germany
  • , Fredericus J. M. van EedenAffiliated withMax-Planck-Institut für Entwicklungsbiologie, Abteilung Genetik, Spemannstrasse 35, D-72076 Tübingen, Germany
    • , Makoto Furutani-SeikiAffiliated withMax-Planck-Institut für Entwicklungsbiologie, Abteilung Genetik, Spemannstrasse 35, D-72076 Tübingen, Germany
    • , Michael GranatoAffiliated withMax-Planck-Institut für Entwicklungsbiologie, Abteilung Genetik, Spemannstrasse 35, D-72076 Tübingen, Germany
    • , M. HammerschmidtAffiliated withMax-Planck-Institut für Entwicklungsbiologie, Abteilung Genetik, Spemannstrasse 35, D-72076 Tübingen, Germany
    • , Carl-Philipp HeisenbergAffiliated withMax-Planck-Institut für Entwicklungsbiologie, Abteilung Genetik, Spemannstrasse 35, D-72076 Tübingen, Germany
    • , Yun-Jin JiangAffiliated withMax-Planck-Institut für Entwicklungsbiologie, Abteilung Genetik, Spemannstrasse 35, D-72076 Tübingen, Germany
    • , D. A. KaneAffiliated withMax-Planck-Institut für Entwicklungsbiologie, Abteilung Genetik, Spemannstrasse 35, D-72076 Tübingen, Germany
    • , R. N. KelshAffiliated withMax-Planck-Institut für Entwicklungsbiologie, Abteilung Genetik, Spemannstrasse 35, D-72076 Tübingen, Germany
    • , Nancy HopkinsAffiliated withMassachusetts Institute of Technology, Center for Cancer Research, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA
    • , Christiane Nüsslein-VolhardAffiliated withMax-Planck-Institut für Entwicklungsbiologie, Abteilung Genetik, Spemannstrasse 35, D-72076 Tübingen, Germany

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Abstract

 Mutations causing a visible phenotype in the adult serve as valuable visible genetic markers in multicellular genetic model organisms such as Drosophila melanogaster, Caenorhabditis elegans and Arabidopsis thaliana. In a large scale screen for mutations affecting early development of the zebrafish, we identified a number of mutations that are homozygous viable or semiviable. Here we describe viable mutations which produce visible phenotypes in the adult fish. These predominantly affect the fins and pigmentation, but also the eyes and body length of the adult. A number of dominant mutations caused visible phenotypes in the adult fish. Mutations in three genes, long fin, another long fin and wanda affected fin formation in the adult. Four mutations were found to cause a dominant reduction of the overall body length in the adult. The adult pigment pattern was found to be changed by dominant mutations in wanda, asterix, obelix, leopard, salz and pfeffer. Among the recessive mutations producing visible phenotypes in the homozygous adult, a group of mutations that failed to produce melanin was assayed for tyrosinase activity. Mutations in sandy produced embryos that failed to express tyrosinase activity. These are potentially useful for using tyrosinase as a marker for the generation of transgenic lines of zebrafish.

Key words Pigment pattern Fin Fish skeleton Tyrosinase Zebrafish