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Characterization of high molecular weight glutenin subunit genes from the Ns genome of Psathyrostachys juncea

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Abstract

The Ns genome of the genus Psathyrostachys possesses superior traits useful for wheat improvement. However, very little is known about the high molecular weight (HMW) subunits of glutenin encoded by the Ns genome. In this paper, we report the isolation of four alleles of HMW glutenin subunit gene from Psathyrostachys juncea. Sequence alignment data shows the four alleles have similar primary structure with those in wheat and other wheat-related grasses, with some unique modifications. All four sequences more closely resemble y-type, rather than x-type, glutenins. However, our results show three of the subunits (1Ns2-4) contain an extra glutamine residue in the N-terminal region not found on typical y-type subunits, as well as the x-type subunit specific sequence LAAQLPAMCRL. These three subunits likely represent an intermediate state in the divergence between x- and y-type subunits. Results also indicate that the Ns genome is more closely related to the St genome of Pseudoroegneria than any other Triticeae genomes.

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References

  • Allaby RG, Banerjee M, Brown TA (1999) Evolution of the high molecular weight glutenin loci of the A, B, D, and G genomes of wheat. Genome 42:296–307

    Article  CAS  PubMed  Google Scholar 

  • Anderson OD, Greene FC (1997) The α-gliadin gene family. II. DNA and protein sequence variation, subfamily structure, and origins of pseudogenes. Theor Appl Genet 95:59–65

    Article  CAS  Google Scholar 

  • Anderson OD, Kuhl JC, Tam A (1996) Construction and expression of a synthetic wheat storage protein gene. Gene 174:51–58

    Article  CAS  PubMed  Google Scholar 

  • Belton PS (1999) On the elasticity of wheat gluten. J Cereal Sci 29:103–107

    Article  CAS  Google Scholar 

  • Blanco A, Resta P, Simeone R, Parmar S, Shewry PR, Sabelli PW, Lafiandra D (1991) Chromosomal location of seed storage protein genes in the genome of Daspyrum villosum (L.) Candargy. Theor Appl Genet 82:358–362

    Article  CAS  PubMed  Google Scholar 

  • Branlard G, Dardevet M (1985) Diversity of grain protein and bread wheat quality. II Correlation between high molecular weight subunits of glutenin and flour quality characteristics. J Cereal Sci 3:345–354

    Article  CAS  Google Scholar 

  • De Bustos A, Rubio P, Jouve N (2000) Molecular characterization of the inactive allele of the gene Glu-A1 and the development of a set of AS-PCR markers for HMW glutenins of wheat. Theor Appl Genet 100:1085–1094

    Article  Google Scholar 

  • Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure from small quantities of fresh leaf tissues. Phytochem Bull 19:11–15

    Google Scholar 

  • Forde J, Malpica J-M, Halford NG, Shewry PR, Anderson OD, Greene FC, Miflin BJ (1985) The nucleotide sequence of a HMW glutenin subunit gene located on chromosome 1A of wheat (Triticum aestivum L.). Nucleic Acids Res 13:6817–6832

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Graveland A, Bosveld P, Lichtendonk WJ, Marseille JP, Moonen JHE, Scheepstra A (1985) A model for the molecular structure of the glutenins from wheat flour. J Cereal Sci 3:1–16

    Article  CAS  Google Scholar 

  • Guo ZF, Yan ZH, Wang JR, Wei YM, Zheng YL (2005) Characterization of HMW prolamines and their coding sequences from Crithopsis delileana. Hereditas 142:56–64

    Article  PubMed  Google Scholar 

  • Jiang QT, Wei YM, Lu ZX, Pu ZE, Lan XJ, Zheng YL (2010) Structural variation and evolutionary relationship of novel HMW glutenin subunits from Elymus glaucus. Hereditas 147:136–141

    Article  PubMed  Google Scholar 

  • Lafiandra D, D’Ovidio R, Porceddu E, Margiotta B, Colaprico G (1993) New data supporting high Mr glutenin subunit 5 as the determinant of quality differences among the pairs 5 + 10 vs 2 + 12. J Cereal Sci 18:197–205

    Article  CAS  Google Scholar 

  • Lawrence GJ, Shepherd KW (1981) Inheritance of glutenin protein subunits of wheat. Theor Appl Genet 60:333–337

    Article  CAS  PubMed  Google Scholar 

  • Li F, Jiang XL, Wei YF, Xia GM, Liu SW (2012) Characterization of a novel type of HMW subunit of glutenin from Australopyrum retrofractum. Gene 492:65–70

    Article  CAS  PubMed  Google Scholar 

  • Liu Z, Yan Z, Wang Y, Liu K, Zheng Y, Wang D (2003) Analysis of HMW glutenin subunits and their coding sequences in two diploid Aegilops species. Theor Appl Genet 106:1368–1378

    CAS  PubMed  Google Scholar 

  • Liu SW, Zhao F, Gao X, Chen FG, Xia GM (2010) A novel high molecular weight glutenin subunit from Australopyrum retrofractum. Amino Acids 39:385–392

    Article  CAS  PubMed  Google Scholar 

  • Liu ST, Zhu XL, Tan Y, Liu SW (2012) Isolation and characterization of Glu-1 genes from the St genome of Pseudoroegneria libanotica. Gene 499:154–159

    Article  CAS  PubMed  Google Scholar 

  • Payne PI, Law CN, Mudd EE (1980) Control by homoeologous group 1 chromosomes of the high-molecular-weight subunits of glutenin, a major protein of wheat endosperm. Theor Appl Genet 58:113–120

    Article  CAS  PubMed  Google Scholar 

  • Payne PI, Nightingale MA, Krattiger AF, Holt LM (1987) The relationship between HMW glutenin subunit composition and the bread making quality of British grown wheat varieties. J Sci Food Agric 40:51–65

    Article  CAS  Google Scholar 

  • Shewry PR, Tatham AS (1997) Disulphide bonds in wheat gluten proteins. J Cereal Sci 25:207–227

    Article  CAS  Google Scholar 

  • Shewry PR, Halford NG, Belton PS, Tatham AS (2002) The structure and properties of gluten: an elastic protein from wheat grain. Philos Trans R Soc Lond B 357:133–142

    Article  CAS  Google Scholar 

  • Shewry PR, Halford NG, Lafiandra D (2003a) Genetics of wheat gluten proteins. Adv Genet 49:111–184

    Article  CAS  PubMed  Google Scholar 

  • Shewry PR, Halford NG, Tatham AS, Popineau Y, Lafiandra D, Belton PS (2003b) The high molecular weight subunits of wheat glutenin and their role in determining wheat processing properties. Adv Food Nutr Res 45:219–302

    Article  CAS  PubMed  Google Scholar 

  • Sun X, Hu SL, Liu X, Qian WQ, Hao ST, Zhang AM, Wang DW (2006) Characterization of the HMW glutenin subunits from Aegilops searsii and identification of a novel variant HMW glutenin subunit. Theor Appl Genet 113:631–641

    Article  CAS  PubMed  Google Scholar 

  • Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol 24:1596–1599

    Article  CAS  PubMed  Google Scholar 

  • Wan Y, Wang D, Shewry PR, Halford NG (2002) Isolation and characterization of five novel high molecular weight subunit of glutenin genes from Triticum timopheevi and Aegilops cylindrica. Theor Appl Genet 104:828–839

    Article  CAS  PubMed  Google Scholar 

  • Wang RRC, Jensen KB (1994) Absence of the J genome in Leymus species (Poaceae: Triticeae): evidence from DNA hybridization and meiotic paring. Genome 37:231–235

    Article  CAS  PubMed  Google Scholar 

  • Yan ZH, Wei YM, Wang JR, Liu DC, Dai SF, Zheng YL (2006) Characterization of two HMW glutenin subunit genes from Taenitherum Nevski. Genetica 127:267–276

    Article  CAS  PubMed  Google Scholar 

  • Zhang HB, Dvorak J (1991) The genome origin of tetraploid species of Leymus (Poaceae: Triticeae) inferred from variation in repeated nucleotide sequences. Am J Bot 78:871–884

    Article  Google Scholar 

Download references

Acknowledgments

This work was supported by funds from the Special Fund for Forest Scientific Research in the Public Welfare (No. 201204402), the Natural Science Foundation of China (No. 31271707; 31000568), and the Independent Innovation Foundation of Shandong University (2012TS009). We thank Dr. Austin Cape for careful reading and language editing.

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Authors and Affiliations

  1. The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, School of Life Sciences, Shandong University, Jinan, 250100, People’s Republic of China

    Lina Kong, Lumin Qin, Guangmin Xia & Shuwei Liu

  2. Shandong Academy of Forestry, Jinan, 250014, People’s Republic of China

    Yu Liang & Lei Sun

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  1. Lina Kong
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  2. Yu Liang
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  3. Lumin Qin
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  4. Lei Sun
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  5. Guangmin Xia
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  6. Shuwei Liu
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Correspondence to Shuwei Liu.

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Communicated by: Sureshkumar Balasubramanian

Lina Kong and Yu Liang contributed equally to this paper.

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Kong, L., Liang, Y., Qin, L. et al. Characterization of high molecular weight glutenin subunit genes from the Ns genome of Psathyrostachys juncea . Dev Genes Evol 224, 189–196 (2014). https://doi.org/10.1007/s00427-014-0477-9

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  • DOI: https://doi.org/10.1007/s00427-014-0477-9

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