Abstract
Main conclusion
29 Moso bamboo VQ proteins were genome-wide identified for the first time, and bioinformatics analysis was performed to investigate phylogenetic relationships and evolutionary divergence. The qRT-PCR data show that PeVQ genes response to different stress treatments.
Accumulating evidence suggests that VQ motif-containing proteins in rice (Oryza sativa), Arabidopsis (Arabidopsis thaliana), and maize (Zea mays) play fundamental roles in response to various biotic and abiotic stresses. However, little is known about the functions of VQ family proteins in Moso bamboo (Phyllostachys edulis). In this study, we performed a genome-wide bioinformatic analysis and expression profiling of PeVQ genes. A total of 29 VQ genes was identified and divided into seven subgroups (I–VII) based on phylogenetic analysis. Gene structure and conserved motif analysis revealed that 25 of 29 VQ genes contained no introns. Multiple sequence alignment showed that Moso bamboo VQ motif-containing proteins contained five variations of the conserved motif. The time of duplication and divergence of Moso bamboo from rice and maize was calculated using K s analysis. A heat map was generated using microarray data from 29 Moso bamboo VQ genes suggesting that these genes were expressed in different tissues or developmental stages. Quantitative real-time PCR (qRT-PCR) and promoter analysis indicated that PeVQ genes were differentially regulated following treatment with polyethylene glycol, abscisic acid and salicylic acid. Our results provide a solid foundation for further research of the specific functions of VQ motif-containing proteins in Moso bamboo.
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Abbreviations
- K a :
-
Number of non-synonymous substitutions per non-synonymous site
- K s :
-
Number of synonymous substitutions per synonymous site
- MYA:
-
Million years ago
- SA:
-
Salicylic acid
- SIB1(2):
-
Sigma factor binding protein 1(2)
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We thank the members of the Laboratory of Modern Biotechnology for their assistance in this study.
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The national natural science foundation of China (31670672) and National Science and Technology Support Program (2015BAD04B03).
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425_2017_2693_MOESM1_ESM.tif
Fig. S1 Expression patterns of 29 VQ genes in Moso bamboo following PEG treatment as determined by qRT-PCR. Mean values and standard deviations (SDs) were obtained from three biological and three technical replicates. The error bars indicate standard deviation. * indicates that the level of expression is significantly different from the value of the control (*P < 0.05, **P < 0.01) (TIFF 188 kb)
425_2017_2693_MOESM2_ESM.tif
Fig. S2 Expression patterns of 29 VQ genes in Moso bamboo following ABA treatment as determined by qRT-PCR. Mean values and standard deviations (SDs) were obtained from three biological and three technical replicates. The error bars indicate standard deviation. * indicates that the level of expression is significantly different from the value of the control (*P < 0.05, **P < 0.01) (TIFF 200 kb)
425_2017_2693_MOESM3_ESM.tif
Fig. S3 Expression patterns of 29 PeVQ genes in response to SA treatment as determined by qRT-PCR. Mean values and standard deviations (SDs) were obtained from three biological and three technical replicates. The error bars indicate standard deviation. * indicates that the level of expression is significantly different from the value of the control (*P < 0.05, **P < 0.01) (TIFF 202 kb)
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Wang, Y., Liu, H., Zhu, D. et al. Genome-wide analysis of VQ motif-containing proteins in Moso bamboo (Phyllostachys edulis). Planta 246, 165–181 (2017). https://doi.org/10.1007/s00425-017-2693-9
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DOI: https://doi.org/10.1007/s00425-017-2693-9