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Genome-wide fungal stress responsive miRNA expression in wheat

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Abstract

MicroRNAs (miRNAs) are small non-coding class of RNAs. They were identified in many plants with their diverse regulatory roles in several cellular and metabolic processes. A number of miRNAs were involved in biotic and abiotic stress responses. Here, fungal stress responsive wheat miRNAs were analyzed by using miRNA-microarray strategy. Two different fungi (Fusarium culmorum and Bipolaris sorokiniana) were inoculated on resistant and sensitive wheat cultivars. A total of 87 differentially regulated miRNAs were detected in the 8 × 15 K array including all of the available plant miRNAs. Using bioinformatics tools, the target transcripts of responsive miRNAs were predicted, and related biological processes and mechanisms were assessed. A number of the miRNAs such as miR2592s, miR869.1, miR169b were highly differentially regulated showing more than 200-fold change upon fungal-inoculation. Some of the miRNAs were identified as fungal-inoculation responsive for the first time. The analyses showed that some of the differentially regulated miRNAs targeted resistance-related genes such as LRR, glucuronosyl transferase, peroxidase and Pto kinase. The comparison of the two miRNA-microarray analyses indicated that fungal-responsive wheat miRNAs were differentially regulated in pathogen- and cultivar-specific manners.

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Abbreviations

B:

Resistant wheat cv. Bezostaja

BC:

Bezostaja control

BI:

Bezostaja inoculated

EST:

Expressed sequence tag

M:

Susceptible wheat cv. Mizrak

MC:

Mizrak control

MI:

Mizrak inoculated

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Acknowledgments

The study was funded by TUBITAK with Grant Number: 113O546.

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Authors and Affiliations

  1. Department of Biology, Faculty of Science, Cankiri Karatekin University, 18100, Cankiri, Turkey

    Behçet Inal, Mine Türktaş, Sezer Okay & Turgay Unver

  2. Department of Biology, Faculty of Arts and Science, Mustafa Kemal University, Hatay, Turkey

    Hakan Eren, Emre Ilhan & Mustafa Erayman

  3. Department of Field Crops, Faculty of Agriculture, Mustafa Kemal University, Hatay, Turkey

    Mehmet Atak

Authors
  1. Behçet Inal
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  2. Mine Türktaş
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  3. Hakan Eren
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  4. Emre Ilhan
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  5. Sezer Okay
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  6. Mehmet Atak
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  8. Turgay Unver
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Correspondence to Turgay Unver.

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B. Inal and H. Eren authors have equally contributed.

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Inal, B., Türktaş, M., Eren, H. et al. Genome-wide fungal stress responsive miRNA expression in wheat. Planta 240, 1287–1298 (2014). https://doi.org/10.1007/s00425-014-2153-8

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  • DOI: https://doi.org/10.1007/s00425-014-2153-8

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