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An integrative analysis of four CESA isoforms specific for fiber cellulose production between Gossypium hirsutum and Gossypium barbadense

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Abstract

Cotton fiber is an excellent model system of cellulose biosynthesis; however, it has not been widely studied due to the lack of information about the cellulose synthase (CESA) family of genes in cotton. In this study, we initially identified six full-length CESA genes designated as GhCESA5–GhCESA10. Phylogenetic analysis and gene co-expression profiling revealed that CESA1, CESA2, CESA7, and CESA8 were the major isoforms for secondary cell wall biosynthesis, whereas CESA3, CESA5, CESA6, CESA9, and CESA10 should involve in primary cell wall formation for cotton fiber initiation and elongation. Using integrative analysis of gene expression patterns, CESA protein levels, and cellulose biosynthesis in vivo, we detected that CESA8 could play an enhancing role for rapid and massive cellulose accumulation in Gossypium hirsutum and Gossypium barbadense. We found that CESA2 displayed a major expression in non-fiber tissues and that CESA1, a housekeeping gene like, was predominantly expressed in all tissues. Further, a dynamic alteration was observed in cell wall composition and a significant discrepancy was observed between the cotton species during fiber elongation, suggesting that pectin accumulation and xyloglucan reduction might contribute to cell wall transition. In addition, we discussed that callose synthesis might be regulated in vivo for massive cellulose production during active secondary cell wall biosynthesis in cotton fibers.

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Abbreviations

CalS:

Callose synthase

CESA:

Cellulose synthase

CrI:

Crystalline index

CSC:

Cellulose synthase complex

DP:

Degree of polymerization

EST:

Expression sequence tag

Gb :

Gossypium barbadense

Gh :

Gossypium hirsutum

Kor:

Korrigan

RACE:

Rapid-amplification of cDNA ends

SGT :

Sterol glycosyltransferase

UBQ :

Ubiquitin

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Acknowledgments

We thank Professors Guozheng Yang and Xianda Yi for kindly providing cotton seeds. This work was supported in part by grants from the 973 Specific Pre-project (2010CB134401), the 973 project (2012CB114500), the National Transgenic Project (2009ZX08009-119B) and the 111 Project (B08032).

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Authors and Affiliations

  1. National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan, 430070, China

    Ao Li, Tao Xia, Wen Xu, Tingting Chen, Xianliang Li, Jian Fan, Ruyi Wang, Shengqiu Feng, Yanting Wang & Liangcai Peng

  2. Biomass and Bioenergy Research Centre, Huazhong Agricultural University, Wuhan, 430070, China

    Ao Li, Tao Xia, Wen Xu, Tingting Chen, Xianliang Li, Jian Fan, Ruyi Wang, Shengqiu Feng, Yanting Wang & Liangcai Peng

  3. College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China

    Ao Li, Xianliang Li, Jian Fan, Ruyi Wang, Shengqiu Feng, Yanting Wang, Bingrui Wang & Liangcai Peng

  4. College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China

    Tao Xia, Wen Xu, Tingting Chen & Liangcai Peng

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  1. Ao Li
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  2. Tao Xia
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  3. Wen Xu
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  4. Tingting Chen
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  5. Xianliang Li
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  8. Shengqiu Feng
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  9. Yanting Wang
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  10. Bingrui Wang
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  11. Liangcai Peng
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Correspondence to Liangcai Peng.

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A. Li and T. Xia contributed equally to the work.

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Li, A., Xia, T., Xu, W. et al. An integrative analysis of four CESA isoforms specific for fiber cellulose production between Gossypium hirsutum and Gossypium barbadense . Planta 237, 1585–1597 (2013). https://doi.org/10.1007/s00425-013-1868-2

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