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Antimycin A effect on the electron transport in chloroplasts of two Chlamydomonas reinhardtii strains

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Abstract

The effects of antimycin A on the redox state of plastoquinone and on electron donation to photosystem I (PS I) were studied in sulfur-deprived Chlamydomonas reinhardtii cells of the strains cc406 and 137c. We found that this reagent suppresses cyclic electron flow around PS I in the cc406 strain, whereas this inhibitory effect was completely absent in the 137c strain. In the latter strain, antimycin A induced rapid reduction of plastoquinone in the dark and considerably enhanced the rate of electron donation to P700 + in the dark. Importantly, neither myxothiazol, an inhibitor of mitochondrial respiration, FCCP, a protonophore, nor propyl gallate, an inhibitor of the plastid terminal oxidase, induced such a strong effect like antimycin A. The results indicate that in the chloroplast of the 137c strain, antimycin A has a site of action outside of the machinery of cyclic electron flow.

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Abbreviations

AA:

Antimycin A

DCMU3:

3-(3,4-dichlorophenyl)-1,1-dimethylurea

CEF:

Cyclic electron flow around PS I

FQR:

Ferredoxin-quinone reductase

Chl:

Chlorophyll

ETC:

Electron transport chain

FCCP:

Carbonylcyanide-p-trifluoromethoxyphenyl-hydrazone

Fd:

Ferredoxin

FNR:

Ferredoxin-NADP reductase

Mxt:

Myxothyazol

MV:

Methyl viologen

NDH:

NADH dehydrogenase

PPFD:

Photosynthetic photon flux density

PQ:

Plastoquinon

Pc:

Plastocyanin

PTOX:

Plastid terminal oxidase

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Acknowledgments

This study was supported by the Russian Federal Target Programme (8077) (TA, GK, TK). ET thanks European Regional Development Fund (Bio Refine Tech program) for financial support.

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Authors and Affiliations

  1. Biological Faculty, Moscow State University, Vorobyevi Gory, 119992, Moscow, Russia

    Taras K. Antal, Galina P. Kukarskikh, Alexander A. Bulychev & Tatyana Krendeleva

  2. Department of Biochemistry and Food Chemistry Molecular Plant Biology, University of Turku, 20014, Turku, Finland

    Esa Tyystjärvi

Authors
  1. Taras K. Antal
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  2. Galina P. Kukarskikh
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  3. Alexander A. Bulychev
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  4. Esa Tyystjärvi
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  5. Tatyana Krendeleva
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Correspondence to Taras K. Antal.

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Antal, T.K., Kukarskikh, G.P., Bulychev, A.A. et al. Antimycin A effect on the electron transport in chloroplasts of two Chlamydomonas reinhardtii strains. Planta 237, 1241–1250 (2013). https://doi.org/10.1007/s00425-013-1843-y

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