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The BIG gene is required for auxin-mediated organ growth in Arabidopsis

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Abstract

Control of organ size by cell expansion and cell proliferation is a fundamental process during development, but the importance of BIG in this process is still poorly understood. Here, we report the isolation and characterization of a new allele mutant of BIG in Arabidopsis: big-j588. The mutant displayed small aerial organs that were characterized by reduced cell size in the epidermis and short roots with decreased cell numbers. The big-j588 axr1 double and big-j588 arf7 arf19 triple mutants displayed more severe defects in leaf expansion and root elongation than their parents, implying BIG is involved in auxin-dependent organ growth. Genetic analysis suggests that BIG may act synergistically with PIN1 to affect leaf growth. The PIN1 protein level decreased in both the root cells and the tips of leaf pavement cell lobes of big-j588. Further analysis showed that the auxin maxima in the roots and the leaves of big-j588 decreased. Therefore, we concluded that the small leaves and the short roots of big-j588 were associated with reduction of auxin maxima. Overall, our study suggested that BIG is required for Arabidopsis organ growth via auxin action.

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Abbreviations

IAA:

Indole-3-acetic acid

NAA:

1-Naphthaleneacetic acid

NPA:

N-1-Naphthylphthalamic acid

PI:

Propidium iodide

X-Gluc:

5-Bromo-4-chloro-3-indolyl β-d-glucuronide

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Acknowledgments

We are grateful to Liping Guan for confocal images, P. Doerner for the CycB1-GUS, T. Guilfoyle for DR5-GUS and J. Friml for DR5-GFP and PIN1-GFP seeds. This work was supported by the Ministry of Agriculture of the People’s Republic of China (Grant NO. 2013ZX08009-003-002, 2009ZX08009-029B), the National Natural Science Foundation of China (NSFC) (Grant NO. 31070247, 91017002, 31271460).

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  1. MOE Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou, 730000, China

    Xiaola Guo, Wenwen Lu, Yurong Ma, Qianqian Qin & Suiwen Hou

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  1. Xiaola Guo
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Correspondence to Suiwen Hou.

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Guo, X., Lu, W., Ma, Y. et al. The BIG gene is required for auxin-mediated organ growth in Arabidopsis. Planta 237, 1135–1147 (2013). https://doi.org/10.1007/s00425-012-1834-4

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