Planta

, Volume 237, Issue 3, pp 771–781

Comparative analysis of barley leaf proteome as affected by drought stress

  • Ahmed Ashoub
  • Tobias Beckhaus
  • Thomas Berberich
  • Michael Karas
  • Wolfgang Brüggemann
Original Article

DOI: 10.1007/s00425-012-1798-4

Cite this article as:
Ashoub, A., Beckhaus, T., Berberich, T. et al. Planta (2013) 237: 771. doi:10.1007/s00425-012-1798-4

Abstract

The adaptive response of Egyptian barley land races to drought stress was analyzed using difference gel electrophoresis (DIGE). Physiological measurements and proteome alterations of accession number 15141, drought tolerant, and accession number 15163, drought sensitive, were compared. Differentially expressed proteins were subjected to MALDI-TOF-MS analysis. Alterations in proteins related to the energy balance and chaperons were the most characteristic features to explain the differences between the drought-tolerant and the drought-sensitive accessions. Further alterations in the levels of proteins involved in metabolism, transcription and protein synthesis are also indicated.

Keywords

Barley Difference gel electrophoresis (DIGE) Drought stress Fv/Fm MALDI-TOF-MS Performance index Proteome analysis 

Abbreviations

2D-PAGE

Two-dimensional polyacrylamide gel electrophoresis

DIGE

Difference gel electrophoresis

Hsp

Heat shock protein

IEF

Isoelectric focusing

RWC

Leaf relative water content

MALDI-TOF-MS

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

PEA

Plant efficiency analyzer

PI

Performance index

PDI

Protein disulfide isomerase

Copyright information

© Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  • Ahmed Ashoub
    • 1
    • 2
    • 4
  • Tobias Beckhaus
    • 3
  • Thomas Berberich
    • 1
  • Michael Karas
    • 3
  • Wolfgang Brüggemann
    • 1
    • 4
  1. 1.Biodiversity and Climate Research Centre (BiK-F)Frankfurt am MainGermany
  2. 2.Agricultural Genetic Engineering Research Institute (AGERI)ARCGizaEgypt
  3. 3.Institute of Pharmaceutical ChemistryJohann Wolfgang Goethe-University FrankfurtFrankfurt am MainGermany
  4. 4.Institute of Ecology, Evolution, and DiversityJohann Wolfgang Goethe-University FrankfurtFrankfurt am MainGermany

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