Thaxtomin A-deficient endophytic Streptomyces sp. enhances plant disease resistance to pathogenic Streptomyces scabies
- First Online:
- Cite this article as:
- Lin, L., Ge, H.M., Yan, T. et al. Planta (2012) 236: 1849. doi:10.1007/s00425-012-1741-8
- 1.1k Downloads
Each plant species in nature harbors endophytes, a community of microbes living within host plants without causing any disease symptom. However, the exploitation of endophyte-based phytoprotectants is hampered by the paucity of mechanistic understandings of endophyte-plant interaction. We here reported two endophytic Streptomyces isolates IFB-A02 and IFB-A03 recovered from a stress-tolerant dicotyledonous plant Artemisia annua L. After the determination of their non-pathogenicity at the genomic level and from the toxin (thaxtomin A, TXT) level, the endophytism of both isolates was supported by their successful colonization in planta. Of the two endophytes, IFB-A03 was further studied for the mechanism of endophyte-conferred phytoprotection owing to its plant growth promotion in model eudicot Arabidopsisthaliana. Using the endophyte-Arabidopsis co-cultivation system into which pathogenic Streptomyces scabies was introduced, we demonstrated that IFB-A03 pre-inoculation could activate the salicylic acid (SA)-mediated plant defense responses upon pathogen challenge. Moreover, IFB-A03 was shown to partially rescue the defense deficiency in eds5 (enhanced disease susceptibility 5) Arabidopsis mutants, putatively acting at the upstream of SA accumulation in the defense signaling pathway associated with the systemic acquired resistance (SAR). These data suggest that endophytic Streptomyces sp. IFB-A03 could be a promising candidate for biocontrol agents against S. scabies—a causative pathogen of common scab diseases prevailing in agronomic systems.
KeywordsArtemisia annua L. Endophyte Streptomyces sp. Arabidopsis thaliana Plant defense
Enhanced disease susceptibility 5
Systemic acquired resistance
Plants in nature encounter a multitude of antagonists and beneficial organisms in their lifetime. While plant responses to stimuli from external bio-environment (such as pathogenic microbes and herbivores) have been the subject of considerable investigation, at least a category of biotic agents existing in plant internal niches has been nearly neglected for decades; however, it is emerging as one of the hottest topics in the field of plant–microbe interaction, which is the so-called endophyte. As a community of harmless microorganisms colonizing inside plant tissues and present in much less amounts than pathogens, endophytes are ubiquitous in terrestrial plants (Tan and Zou 2001; Arnold et al. 2003; Thomas and Soly 2009). Some of them are believed to confer beneficial effects by promoting plant growth and enhancing plant adaptability to environmental stresses during long-time coevolution, which are exemplified by the grass-inhabiting fungal endophyte Neotyphodium sp. (Tan and Zou 2001), rice-residing endophytic bacterium Pantoea agglomerans (Feng et al. 2006), and some N2-fixative bacteria such as Gluconacetobacter diazotrophicus and Serratia marcescens endophytic to the sugarcane and rice, respectively (Pan and Vessey 2001; Gyaneshwar et al. 2001). Despite increasingly growing evidence for fungal and bacterial endophytes, no endophytic actinobacteria were reported until the first Streptomyces sp. was isolated from a grass Lolium perenne (Guerny and Mantle 1993). Subsequent discoveries of endophytic Streptomyces spp. indicate that they can be novel sources of bioactive metabolites including antibiotics, as underpinned by a series of elaborate work from Strobel’s group (Castillo et al. 2002, 2003; Ezra et al. 2004). In contrast to soil-habitating actinobacteria, some of which develop incompact relationships with plant roots (known as rhizospheric species), a portion of actinobacteria may colonize inside the plant tissues and thus establish intimate associations with the host, which are termed endophytic actinobacteria, as exemplified by Frankia spp. distinctly forming nitrogen-fixative nodules in roots (Siciliano et al. 1998).
On the other hand, phytopathogenic actinobacteria, though constituting only a minority of plant-associated actinobacteria, may result in damage to plant cultivation and crop yields (Lerat et al. 2009). The representative pathogenic species are known to be Streptomyces scabies, S. acidiscabies, and S. turgidiscabies, causing common scab diseases in potato and some vegetables (King et al. 1991; Loria et al. 1995, 1997). Their pathogenicity has been ascribed to the biosynthesis and secretion of thaxtomin A (TXT), a phytotoxin which may result in dramatic cell swelling, reduced seedling growth, and inhibition of cellulose synthesis in plants as exemplified in Arabidopsis thaliana (Scheible et al. 2003). The TXT biosynthesis genes including nos gene are reported to reside on a pathogenicity island (PAI), which is conserved and horizontally transmissible among different species of the Genus Streptomyces, thereby accounting for the emergence of new pathogenic Streptomyces spp. in agronomic cultivation systems (Healy et al. 1999; Bukhalid et al. 1998). In addition to the well-known virulence factor gene nec1 (Bukhalid et al. 1998; Loria et al. 1995), the nos gene, which encodes nitric oxide synthase (NOS) essential to the ultimate nitration during TXT biosynthesis, represents another key PAI determinant in pathogenic streptomycetes (Kers et al. 2004).
Early in 1929, McKinney proposed a resistance phenomenon defined later as “cross-protection” based on the observation that tobacco plants pre-inoculated with mild virus strains might protect those plants from attack by a virulent strain of tobacco mosaic virus (TMV) (MacKenzie and Tremaine 1990). Despite the promising potential, the application of “cross-protection” became a dilemma primarily owing to the suspicion that mild virus strains protective for one plant species may cause serious diseases on the other(s) (Fulton 1986; Jackson and Talor 1996). The plant systemic acquired resistance (SAR) was subsequently discovered as indicated by an enhanced resistance to secondary pathogenic challenge in distal (systemic) tissues of tobacco after pre-inoculation with TMV (Durrant and Dong 2004). The scope of SAR has been broadened because it can be activated by necrosis-causing pathogens and some non-pathogenic rhizobacteria, and the evoked resistance is long-term and effective in systemic tissue against wide-spectrum pathogens including viruses, bacteria, and fungi (Conrath et al. 2002; Durrant and Dong 2004). The establishment of plant SAR requires an elevation in endogenous salicylic acid (SA) level, and its onset is associated with the expression of many pathogenesis-related (PR) genes such as PR-1, and PR-5 (Durrant and Dong 2004).
In view of a paucity in the reports regarding endophytic actinobacteria other than Frankia spp., and the necessity to investigate the interaction between plant and Streptomyces spp. as pathogenic streptomycetes are threatening worldwide crop/vegetable productions (Lerat et al. 2009), this study initiates from the isolation and characterization of endophytic streptomycetes from a highly stress-tolerant herb Artemisia annua L.. The selection of A. annua for isolating actinobacterial endophytes is based on two reasons: (1) it has been ethnobotanically used as a widely accepted folk medicine for its antimalaria, anti-schistosomiasis, antitussis, expectoration, and antiasthma activities in Asia (Tan et al. 1998; Bhakuni et al. 2001); (2) some of the plant stress-tolerant and/or medicinal properties are believed to result from the endophytes habitating inside (Castillo et al. 2002; Kuffner et al. 2010; Strobel and Daisy 2003), which can also explain the well-known notion that the low stress tolerance of axenic plants is ascribed, at least in part, to the absence of endophytic microbes (Hallmann et al. 1997). Historically, endophytes have been considered as weakly virulent or “latent” pathogens to plants (Hallmann et al. 1997). However, recent studies have demonstrated that at least some of them play beneficial roles in plant development and health, such as plant growth promotion and alleviation of disease symptoms caused by plant pathogens (Hallmann et al. 1997; Hasegawa et al. 2006). Since most plant-associated actinobacteria are non-pathogenic (Lerat et al. 2009), the protection of plants from pathogenic Streptomyces sp. could be expected from either endophytic Streptomyces-produced antimicrobials and/or the pre-colonization of the endophyte (Tokala et al. 2002; Cao et al. 2005; Hasegawa et al. 2006).
In this study, two culturable endophytic streptomycetes were isolated from the aerial tissues of healthy Artemisia annua L., which possesses the stress tolerance to chilling, drought, and pest attacks. Our attention was paid to actinobacterial endophytes because (1) studies regarding actinomycetes endophytic to dicotyledonous plants (particularly A. annua) are spare, and the work afforded by Strobel’s group and Zhao et al. (2010) were focused on antibiotics production and microbial taxonomic status, respectively, rather than the mechanism of the endophyte interacting with host plant defense responses; (2) neither IFB-A02 nor IFB-A03 possesses pathogenicity when re-introduced into A. annua (original host) in the greenhouse (Fig. S2); and (3) they can enter and colonize into the alternative host Arabidopsis thaliana.
Given that S. scabies is a primary phytopathogen which is the potential PAI donor species among the genus Streptomyces in nature (Lerat et al. 2009), and also encouraged by Franco’s previous work relating to wheat endophytes (Conn et al. 2008), we subsequently investigated the presumable phytoprotective effects of endophytic Streptomyces using model plant Arabidopsis thaliana, a member of dicotyledonous plants which Artemisia annua L. belongs to. We further elucidated the molecular mechanism underlying endophyte-plant interaction in response to S. scabies attack with an additional intention to determine whether plant defensive hormones SA and/or jasmonic acid (JA) participate in the main signaling pathways associated with enhanced plant resistance. The results herein demonstrate that pre-inoculation of endophytic Streptomyces sp. IFB-A03 may activate the SA-mediated defense responses in Arabidopsis thaliana, putatively acting toward the upstream of SA accumulation in the SA signaling pathway leading eventually to SAR.
Materials and methods
Isolation of endophytic actinobacteria
The plants of Artemisia annua L. were collected from the fields of Nanjing suburb (Sept. 2007) and Tianmu Lake (Mar. 2008), Jiangsu Province, P. R. China. The plant aerial tissues (stems and leaves) were thoroughly cleaned, surface sterilized as described in Coombs and Franco (2003), and immediately ground in 5 ml of sterile physiologic saline (pH 7.0), followed by the serial dilution plating onto the modified Gause No. 1 synthetic media supplemented with kanamycin (25 μg ml−1) and nystatin (25 μg ml−1) (referred to as the isolation media). All plates were incubated at 27–28 °C for up to 4 weeks. Actinomycete colonies were examined using a stereoscope (LED RING Illuminator JSZ6D, China) and picked based on morphological features and colors of pigmentation.
To validate the efficacy of the surface sterilization protocol, the last-run rinsing water was plated and incubated onto the same isolation media as did each plant specimen, and surface-sterilized plant tissue fragments were rolled onto the surface of isolation plates and incubated equally.
Genomic DNA was extracted from the 48-h-cultured mycelia of each endophytic isolate as described in Wang et al. (1996). The 16S rDNAs of two isolates were PCR amplified using actinobacterial universal primers (Table S1) and sequenced. Phylogenetic analyses based on 16S rDNA sequences were done by searching Genbank by means of BLAST and then confirmed by performing the multiple sequence alignment, and the tree was constructed by means of the neighbor-joining (NJ) method in Mega 4.0 (Tamura et al. 2007). The stability of tree topology was evaluated by means of BOOTSTRAP based on 1,000 pseudoreplications. The 16S rRNA gene sequences for the two endophytic actinobacterial isolates IFB-A02 and IFB-A03 have been deposited in the GenBank database (accession no. HQ317204-317205).
Microscopic examination of endophytes in planta
The roots and stems of 6- or 7-week-old endophyte-pre-inoculated Arabidopsis plantlets were subjected to sample preparation (See Supplementary Material). Ultra-thin sections of 60–70 nm were examined under an H-7560 transmission electron microscope (TEM, Hitachi, Japan) operating at 80 kV.
Pre-treatment of Arabidopsis aseptic seedlings with endophyte
The endophyte-Arabidopsis co-cultivation system was established at the seed phase or early seedling stage. To optimize its establishment, surface-sterilized seeds were inoculated comparatively by (1) treating with the spore suspension (2 × 108 spores ml−1) for 1.5–2 h followed by uniform sowing on MS agar, and (2) sowing on the sterile MS agar to germinate into 4–6 leafed seedlings that were transferred to the sterile jars with the pre-loaded MS agars containing 200 μl of spore suspension (2 × 108 spores ml−1). The seedlings were cultivated until 5-weeks old as described in the “Cultivation of the sterile Arabidopsis seedlings” of Supplementary Material. The two protocols gave similar efficacy (Fig. S4).
SA application of Arabidopsis
Six-week-old Arabidopsis plants were sprayed with 2 mM SA at two lower leaves 3 days before challenge of pathogen, Streptomycesscabies ACCC 41024 (a phytopathogen preserved at Agricultural Culture Collection of China, Beijing, referred to as S. scabies hereafter unless otherwise stated), and the systemic leaves were harvested at 48 h post challenge. Control-treated plants were applied with sterile distilled H2O.
Pathogen challenge of endophyte-preinoculated Arabidopsis
Following initial pre-inoculation with the endophyte, pathogenic infection was performed with S. scabies using vertical petri dish system as described in Lehr et al. (2008) with some modifications. One half of petri dish was cast by adding the carbon source-free ISP4 agar media (15–20 mL, pH 5.8), while leaving the other half empty. The 5-week-old seedlings pre-inoculated with IFB-A02 and IFB-A03 were transferred onto ISP4 plates. After 6 days, the roots of endophyte-inoculated and -uninoculated Arabidopsis seedlings were challenged with 200 μl of S. scabies spore suspension (1 × 108 spores ml−1), while an equal volume of sterile distilled water was supplied to the control. Leaves were harvested 5 days post challenge and immediately frozen in liquid nitrogen for followup RNA and/or protein extractions.
Semi-quantitative RT-PCR analyses of defensive gene
The extracted RNAs (as detailed in Supplementary Material) from the plant materials tested were converted into first-strand cDNA using PrimeScript™ Reverse Transcriptase kit (Takara Bio., Dalian, China) following the manufacturer’s protocol. One microliter of cDNA was amplified for PR-1, PR-5, and/or PDF1.2 gene using SYBR®Premix ExTaq™ (Takara Bio., Dalian, China) with specific primers outlined in Table S1. Arabidopsis housekeeping gene Actin was used as an internal control. PCR products were subjected to gel electrophoresis and images recorded. Representative results from two independent experiments are shown.
Protein expression analyses
The protein extracts (20 μg) were separated by SDS-PAGE and transferred to a nitrocellulose membrane (Millipore, USA). For immunoblotting, the membranes were incubated with a rabbit polyclonal anti-PR1 antibody followed by incubation with horseradish peroxidase (HRP)-conjugated anti-IgG antibody raised in goat (KeyGEN, Nanjing, China). Specific protein bands were detected using ECL western-blotting kit (Amersham Biosci., UK) and images recorded.
Assays for defensive traits of Arabidopsis
Peroxidase activity was examined as described in “Supplementary Material.” The in-planta release of reactive oxygen species (ROS), primarily superoxide (O2−.), was detected via nitroblue tetrazolium (NBT) method (Tanaka et al. 2006). The leaves of endophytic spore-inoculated and -uninoculated Arabidopsis were collected at 2 h post pathogen challenge and trimmed, followed by vacuum infiltration and incubation (1 h) in the sodium phosphate buffer (0.05 M, pH 7.5) containing 0.05 % NBT and finally immersed in ethanol. The NBT-stained specimens were imaged by a compound microscope (Zeiss, Germany).
Measurement of plant endogenous salicylic acid (SA)
Fresh systemic leaves were collected from 5-week-old IFB-A03-pretreated and -untreated eds5 Arabidopsis mutants after the pathogenic S. scabies attack, and immediately liquid nitrogen frozen with each specimen consisting of 300 mg (fresh weight) of leaf tissues. Sample preparation followed the described protocol with slight modifications (Engelberth et al. 2003). After dissolved in 1 ml of anhydrous methanol, the SA in the residue derived from each sample was determined as reported (Tamaoki et al. 2008) with its authentic material at 0.5 μg ml−1 as a standard by LC–MS on the instrument (see above) using aqueous methanol (10 % → 100 % within 12 min) as the mobile phase. The data were compared in Fig. 7.
Others including microbial culture, cultivation of the sterile Arabidopsis seedlings, assay of key PAI determinants (nos and nec1), bioassay of phytotoxin TXT, sample preparation for transmission electron microscopy (TEM), peroxidase activity assay, 1H-NMR analysis of microbial culture supernatant of endophyte, RNA extraction, and primers for PCR are detailed in “Supplementary Material.”
Results and discussion
Isolation and identification of Streptomyces spp. IFB-A02 and IFB-A03
Endophytism of IFB-A02 and IFB-A03
As for the two isolates IFB-A02 and -A03, their genomic absence of nec1 and inability to produce TXT are in accord with the results by Coombs and Franco (2003) concerning endophytes from wheat roots. In contrast to the thin-layer-chromatography (TLC) technique in Coombs and Franco (2003), this study used LC–ESI–MS analysis with more accuracy for detecting toxin TXT at trace level. Notwithstanding the plant sources of endophytic isolation and phylogenetic position of endophytes streptomycetes, the lack of nec1 in the genome and of TXT production at the chemical level may be served as in vitro markers for differentiating endophytic streptomycetes from pathogenic ones, of which S. scabies is the representative. Therefore, successful in-planta colonization of IFB-A02 and -A03 as aforedescribed, along with the results of in vitro assays, has provided corroborant evidence for their endophytism.
The endophytic IFB-A02 and IFB-A03 were tested for their effects on plant using model eudicot Arabidopsis thaliana. Our preliminary work showed that the culture filtrates of both endophytes, when applied to the seeds, could promote the seedling growth and plant disease resistance (Fig. S1). The in vivo investigation concerning how IFB-A02 and IFB-A03 affect plant physiological traits was conducted by co-cultivation of the respective strain with Arabidopsis from the seed phase. To our surprise, co-cultivation of Arabidopsis with IFB-A03 was advantageous over that of IFB-A02 in terms of the germinating vigor, first leaf emergence and biomass of mature plants (Fig. S1). This could be stemmed from the differential affinity to the alternative host of the two phylogenetically different endophytes (Fig. 1c), which underpinned as well their difference in generating ROS and modulating peroxidase activity as stated below.
Improved defensive capacity of Arabidopsis by endophytic pre-inoculations
Following the disclosure of endophytism and differential benefits of IFB-A02 and IFB-A03 to the alternative host, whether endophytic pre-inoculation may affect defense responses of Arabidopsis thaliana to pathogenic S. scabies was investigated. The plant defense responses are manifold including structural enhancements like cell wall reinforcement, callose deposition and epidermal trichome induction, and metabolic/cellular regulations such as production of phytoalexins, oxidative burst, modulation in peroxidase activity and as well as transcriptional/expressional changes in defensive genes associated with defense signaling pathways that are mediated by phytohormone SA and/or JA (van Loon et al. 2006; Lin and Tan 2011).
This was assayed in endophyte-preinoculated and -uninoculated intact Arabidopsis leaves challenged equally by S. scabies. As shown in Fig. S5, pre-inoculation of Arabidopsis with IFB-A02 evoked more increases in peroxidase activity in leaves than that with IFB-A03 as compared to un-inoculated control (2.3-folds vs. 1.8-folds). Peroxidase plays an important role in regulating the ROS level during plant defense responses and conferring resistance to wide-spectrum pathogens (Bindschedler et al. 2006; Wojtaszek 1997). The discerned increment in peroxidase activity suggested that the endophyte pre-inoculation may potentiate the alternative host’s resistance to invasive pathogen by activating on the defense-related enzyme.
While a microburst of ROS contributes to the SAR state by activating defense responses at a low level throughout the plant (Durrant and Dong 2004), an elevated ROS burst to a relatively greater extent (exceeding the threshold) may be harmful owing to the resultant oxidative damage to DNA, lipids, and proteins (Rodriguez et al. 2008), thereby driving the cells to programed death. The distinct difference between the two endophytes in affecting peroxidase activity and ROS release was in accord with phenotypic observations that the IFB-A02-preinoculated seedlings was inferior in growth to those treated with IFB-A03. Accordingly, our further investigation was performed principally with IFB-A03 in view of its acceptable phytoprotective effects for host. Moreover, its spore-bearing feature would add to its application potential as biopesticides.
Pre-inoculation with IFB-A03 induces SAR-related gene transcripts in Arabidopsis
SA involvement in IFB-A03-activated defense responses in Arabidopsis
To further elucidate the effects of IFB-A03 inoculation on Arabidopsiseds5 mutants in response to pathogen, transcripts of defensive genes PR-1, PR-5, and PDF1.2 were analyzed in the systemic leaves of the IFB-A03-inoculated seedlings of both wild-type (WT) and eds5-mutated Arabidopsis in comparison to their un-inoculated counterparts. As a result, IFB-A03-inoculated eds5 exhibited upregulated transcripts of SAR-related genes PR-1 and PR-5 (Fig. 6c) though to a lesser extent than those observed with the IFB-A03-treated WT. Endophyte-inoculated eds5 also displayed the induced transcript of PDF1.2—a marker gene in JA signaling pathway (Fig. 6c). These observations for IFB-A03-inoculated eds5, which stand as opposed to those documented for eds5 mutants, suggest that IFB-A03 pre-inoculation may partially “rescue” SA-deficient defense pathway in eds5 mutants of Arabidopsis. In addition, the induction of PDF1.2 transcripts implies that JA signaling pathway may be operative to offset the defense responses that are “partially complemented” by the interactions of IFB-A03 inoculants with eds5 Arabidopsis. As suggested by Conn et al. (2008), the streptomycetes may be perceived by plant as “minor” pathogens to trigger an array of defense responses.
Endophytic IFB-A03 acts toward upstream of SA accumulation
As reviewed by Schrey and Tarkka (2008), plants enhance their resistance against pathogen ingress through a versatile array of local or systemic defense responses, involving (1) local defenses by structural enhancement of plant cell walls, (2) streptomyte-induced systemic plant resistance by activating SA and JA/ethylene signaling networks, and (3) plant growth promotion correlated with its suppression of pests. Our results indicate that at least two mechanisms, (2) and (3) as mentioned above, may be implicated in endophytic streptomycete-conferred phytoprotection. Mechanism (2) is distinct in light of our data regarding SA accumulation and upregulation of PR transcripts while mechanism (3) acts indirectly to promote plant growth through enhancing the disease resistance against pests, as shown in Figs. S1 and S2. However, the plant-streptomycetes interaction mechanisms may vary with different plant and Streptomyces species. It has been found that the alteration in spruce root architecture is one of mechanism underlying the enhanced disease resistance following streptomycete inoculation (Lehr et al. 2008), referring to mechanism (1) (Schrey and Tarkka 2008).
This study addressed or at least suggested a key event ahead of SA signal accumulation during the SAR pathway in the model plant (Fig. S8). Desired is full revelation of the plant defense signaling component (gene or gene regulator) with which endophytic IFB-A03 interacts to gain more insights into endophyte-plant mutualism.
Streptomycetes are abundant producers of various secondary metabolites such as plant growth regulators, antimicrobials, and siderophores (Conn et al. 2008). From a morphological aspect, they possess such specialized structures as spores which facilitate their survival in nature, as well as in-planta colonization, leading to an advantageous mode of colonization over sessile bacteria. These features of streptomycetes add to their applicative values for biologic control. Previous studies have showed that inoculation of wheat with the endophytic Streptomyces sp. strain EN27 may promote growth and enhance disease resistance both in vitro and in-planta (Coombs 2002).
Previous studies (Conn et al. 2008) demonstrated the involvement of SA in the plant resistance endowed by Streptomyces sp. EN27 in response to pathogenic E. carotovora subsp. Carotovora, operating via an NPR1-independent signaling pathway, based on the data that strain EN27-treated npr1-1 mutants infected with E. carotovora subsp. Carotovora displayed the induction of PR-1, PDF1.2, and the plants showed little disease symptom. Our study indicated that endophytic Streptomyces sp. IFB-A03 may activate the SA-dependent signaling in Arabidopsis thaliana, thereby conferring plant resistance against pathogenic S. scabies. Further investigation is needed to address whether the IFB-A03-activated signaling is NPR1 dependent or not.
In summary, we show here the validation of the ethnobotanical path for recovering the plant defensive microbes such as the newly recognized endophytic Streptomyces sp. IFB-A03 sourced from the stress-adaptive herb Artemisia annua L. The disease resistance endowed by this endophyte is reflected unequivocally by the pre-inoculation test to display, in the alternative host Arabidopsis thaliana, its strengthening effect to retard subsequent infection of pathogenic S. scabies. Concerning its mode of action, IFB-A03 may stimulate plant defenses by acting upstream of SA accumulation in the SA-dependent signaling pathway. While adding renewed information to the feasibility of endophytism-based “cross-protection,” the present work addresses collectively that the endophytic Streptomyces sp. IFB-A03 is a promising candidate for eco-friendly biocontrol agents. As strengtheners of plant defenses, the endophytic streptomycetes merit further consideration as the missing force in the disease resistance against pathogenic Streptomyces sp.
We thank Profs S. Lu (Nanjing Univ.) and D. T. Ren (China Agric. Univ.) for providing seeds of wild-type Col-0 and eds5 mutant, NahG transgenic Arabidopsis thaliana, respectively, and Prof. Z. Hong (Nanjing Univ.) for helpful suggestions on the manuscript. We are also grateful to the two anonymous referees for their comments and advice for the improvement of manuscript. This work was co-financed by grants from NSFC (30821006 & 90813036) and MOST (2009ZX09501-013).
Conflict of interest
The authors declare that they have no conflict of interest.