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Function of a novel GDSL-type pepper lipase gene, CaGLIP1, in disease susceptibility and abiotic stress tolerance

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Abstract

GDSL-type lipase is a hydrolytic enzyme whose amino acid sequence contains a pentapeptide motif (Gly-X-Ser-X-Gly) with active serine (Ser). Pepper GDSL-type lipase (CaGLIP1) gene was isolated and functionally characterized from pepper leaf tissues infected by Xanthomonas campestris pv. vesicatoria (Xcv). The CaGLIP1 protein was located in the vascular tissues of Arabidopsis root. The CaGLIP1 gene was preferentially expressed in pepper leaves during the compatible interaction with Xcv. Treatment with salicylic acid, ethylene and methyl jasmonate induced CaGLIP1 gene expression in pepper leaves. Sodium nitroprusside, methyl viologen, high salt, mannitol-mediated dehydration and wounding also induced early and transient CaGLIP1 expression in pepper leaf tissues. Virus-induced gene silencing of CaGLIP1 in pepper conferred enhanced resistance to Xcv, accompanied by the suppressed expression of basic PR1 (CaBPR1) and defensin (CaDEF1) genes. The CaGLIP1 lipase produced in Escherichia coli hydrolyzed the substrates of short and long chain nitrophenyl esters. The CaGLIP1-overexpressing Arabidopsis exhibited enhanced hydrolytic activity toward short and long chain nitrophenyl ester, as well as enhanced susceptibility to the bacterial pathogen Pseudomonas syringae pv. tomato and the biotrophic oomycete Hyaloperonospora parasitica. SA-induced expression of AtPR1 and AtGST1, also was delayed in CaGLIP1-overexpressing plants by SA application. During seed germination and plant growth, the CaGLIP1 transgenic plants showed drought tolerance and differential expression of drought- and abscisic acid (ABA)-inducible genes AtRD29A, AtADH and AtRab18. ABA treatment differentially regulated seed germination and gene expression in wild-type and CaGLIP1 transgenic Arabidopsis. Overexpression of CaGLIP1 also regulated glucose- and oxidative stress signaling. Together, these results indicate that CaGLIP1 modulates disease susceptibility and abiotic stress tolerance.

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Abbreviations

ABA:

Abscisic acid

CaMV:

Cauliflower mosaic virus

MeJA:

Methyl jasmonate

MV:

Methyl viologen

SA:

Salicylic acid

SNP:

Sodium nitroprusside

VIGS:

Virus-induced gene silencing

Xcv :

Xanthomonas campestris pv. vesicatoria

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Acknowledgments

This research was supported by a grant (CG1133) from the Crop Functional Genomics Center of the twenty-first Century, the Frontier Research Program funded by the Ministry of Science and Technology of Republic of Korea, a grant from the Center for Plant Molecular Genetics and Breeding Research, Seoul National University, Korea and a grant (20070401034028) from the BioGreen 21 Program, Rural Development Administration, Korea. We thank Dr. S. P. Dinesh-Kumar for the pTRV1 and pTRV2 vectors, and Dr. Kay Gillian for critically reading the manuscript.

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  1. Jeum Kyu Hong

    Present address: Bio-Evaluation Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Ochang, Chungbuk, 363-883, South Korea

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  1. Laboratory of Molecular Plant Pathology, College of Life Sciences and Biotechnology, Korea University, Anam-dong, Sungbuk-ku, Seoul, 136-713, South Korea

    Jeum Kyu Hong, Hyong Woo Choi, In Sun Hwang, Dae Sung Kim, Nak Hyun Kim, Du Seok Choi, Young Jin Kim & Byung Kook Hwang

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Additional information

The nucleotide sequence data reported here has been deposited in the GenBank database under the accession number AY775336.

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Hong, J.K., Choi, H.W., Hwang, I.S. et al. Function of a novel GDSL-type pepper lipase gene, CaGLIP1, in disease susceptibility and abiotic stress tolerance. Planta 227, 539–558 (2008). https://doi.org/10.1007/s00425-007-0637-5

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