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Biophysical consequences of remodeling the neutral side chains of rhamnogalacturonan I in tubers of transgenic potatoes

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Abstract

Two lines of transgenic potato (Solanum tuberosum L.) plants modified in their cell wall structure were characterized and compared to wild type with regard to biomechanical properties in order to assign functional roles to the particular cell wall polysaccharides that were targeted by the genetic changes. The targeted polymer was rhamnogalacturonan I (RG-I), a complex pectic polysaccharide comprised of mainly neutral oligosaccharide side chains attached to a backbone of alternating rhamnosyl and galacturonosyl units. Tuber rhamnogalacturonan I molecules from the two transformed lines are reduced in linear galactans and branched arabinans, respectively. The transformed tuber tissues were found to be more brittle when subjected to uniaxial compression and the side-chain truncation was found to be correlated with the physical properties of the tissue. Interpretation of the force–deflection curves was aided by a mathematical model that describes the contribution of the cellulose microfibrils, and the results lead to the proposition that the pectic matrix plays a role in transmitting stresses to the load-bearing cellulose microfibrils and that even small changes to the rheological properties of the matrix have consequences for the biophysical properties of the wall.

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Abbreviations

RG-I (II) :

Rhamnogalacturonan I (II)

T13.1 :

Transformant with truncated galactan side chains of RG-I

T7.2 :

Transformant with truncated arabinan side chains of RG-I

WT :

Wild type

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Acknowledgements

Vibeke Strange, Morten Laessøe Stephensen and Dorthe Christiansen are acknowledged for skillful technical assistance. Dr. Peter M. Ray is acknowledged for sharing his insights in cell wall biology and inspiring discussions while this manuscript was prepared. Søren Balling Engelsen is acknowledged for introducing us to the Mettler Moisture Analyzer. This investigation was supported by the Danish National Research Foundation. The Danish Research Agency is acknowledged for funding Michael Skjøt, and the work at Silsoe Research Institute was funded by SRI’s competitive strategic grant from the UK Biotechnology and Biological Sciences Research Council.

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Authors and Affiliations

  1. Biotechnology Group, Danish Institute of Agricultural Sciences, Thorvaldsensvej 40, 1871, Frederiksberg C, Denmark

    Peter Ulvskov, Bodil Jørgensen, Michael Skjøt & Bernhard Borkhardt

  2. Department of Dairy and Food Science and Centre for Advanced Food Studies, The Royal Veterinary and Agricultural University, Rolighedsvej 30, 1958, Frederiksberg C, Denmark

    Helle Wium & Karsten Bruun Qvist

  3. Biophysics Group, Silsoe Research Institute, Wrest Park, Silsoe, Bedford, MK45 4HS, UK

    David Bruce & David Hepworth

  4. Plant Biochemistry Laboratory, Department of Plant Biology, Royal Veterinary & Agricultural University, Thorvaldsensvej 40, 1871, Frederiksberg C, Denmark

    Susanne Oxenbøll Sørensen

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  1. Peter Ulvskov
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  2. Helle Wium
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  3. David Bruce
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Correspondence to Peter Ulvskov.

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Ulvskov, P., Wium, H., Bruce, D. et al. Biophysical consequences of remodeling the neutral side chains of rhamnogalacturonan I in tubers of transgenic potatoes. Planta 220, 609–620 (2005). https://doi.org/10.1007/s00425-004-1373-8

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  • DOI: https://doi.org/10.1007/s00425-004-1373-8

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