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Regional adaptation of collagen in skeletal muscle to repeated bouts of strenuous eccentric exercise

  • Muscle physiology
  • Published:
Pflügers Archiv - European Journal of Physiology Aims and scope Submit manuscript

Abstract

This study investigated the injured region-specific alterations of factors related to the “repeated bout effect” (RBE), i.e., when the first bout of eccentric exercise generates resistance to injuries from the second bout of the same exercise. Wistar rats were divided into single injury (SI) and repeated injury (RI) groups. The right gastrocnemius muscle was subjected to a bout of eccentric contractions (ECs) at the age of 14 weeks in the SI group and 10 and 14 weeks in the RI group. The number of injured fibers after the last bout of ECs was lower in RI than in SI. In the SI group, injured fibers after ECs were mainly located in the superficial region of muscle and expressed myosin heavy chain (MHC) IIx and IIb. Prior to the second bout of ECs, the fiber-type composition in the RI group showed decreased MHC IIx and IIb fibers and increased MHC IIa fibers compared with those in the SI group. However, most regenerating fibers showed either MHC IIx or IIb expression. Heat shock protein 72 and total collagen contents in whole muscle were higher in the RI group than in the SI group; however, only the collagen expression in the RI group was more intense than that in the SI group in the superficial region of muscle. These findings suggest that increased collagen may play a more important role in the injured region of muscle than the other factors in RBE.

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Acknowledgments

This study was supported by a Grant-in-Aid for Scientific Research (B) from the Japan Society for the Promotion of Science (no. 15H03078). The authors declare no conflicts of interest and that the results of this study are presented clearly, honestly, and without fabrication, falsification, or inappropriate data manipulation. The results of the present study do not constitute endorsement by the Federation of European Physiological Societies.

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Authors and Affiliations

  1. Department of Life Sciences, The University of Tokyo, 3-8-1 Komaba, Meguro-ku, 153-8902, Japan

    Ryo Takagi & Naokata Ishii

  2. Department of Life and Materials Engineering, Nagoya Institute of Technology, Aichi, Japan

    Riki Ogasawara

  3. Graduate School of Health and Sport Science, Nippon Sport Science University, Tokyo, Japan

    Arata Tsutaki & Koichi Nakazato

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  1. Ryo Takagi
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  2. Riki Ogasawara
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  3. Arata Tsutaki
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  4. Koichi Nakazato
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  5. Naokata Ishii
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Correspondence to Ryo Takagi.

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This study was approved by the ethical committee of the Nippon Sport Science University (no. 014-A03).

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Takagi, R., Ogasawara, R., Tsutaki, A. et al. Regional adaptation of collagen in skeletal muscle to repeated bouts of strenuous eccentric exercise. Pflugers Arch - Eur J Physiol 468, 1565–1572 (2016). https://doi.org/10.1007/s00424-016-1860-3

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  • DOI: https://doi.org/10.1007/s00424-016-1860-3

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