European Journal of Applied Physiology

, Volume 113, Issue 10, pp 2473–2486

Swimming exercise training-induced left ventricular hypertrophy involves microRNAs and synergistic regulation of the PI3K/AKT/mTOR signaling pathway

Authors

  • Zhichao Ma
    • Physical Education CollegeYangzhou University
  • Jie Qi
    • Physical Education CollegeYangzhou University
  • Shuai Meng
    • Physical Education CollegeYangzhou University
  • Baoju Wen
    • Physical Education CollegeYangzhou University
    • Physical Education CollegeYangzhou University
Original Article

DOI: 10.1007/s00421-013-2685-9

Cite this article as:
Ma, Z., Qi, J., Meng, S. et al. Eur J Appl Physiol (2013) 113: 2473. doi:10.1007/s00421-013-2685-9

Abstract

Purpose

Swimming exercise leads to a nonpathological, physiological left ventricular hypertrophy. However, the potential molecular mechanisms are unknown. We investigated the role of microRNAs (miRNA) regulating the cardiac signal cascades were studied in exercised rats.

Methods

Female Wistar rats were assigned into two groups: (1) sedentary control (SC), (2) swimming exercise (SE). The rats in the SE group completed a 1-h swimming exercise, 5 times/week/8-week with 5 % body overload. miRNA, phosphoinositide-3-kinase catalytic alpha polypeptide (PIK3α), phosphatase and tensin homolog (PTEN) and tuberous sclerosis complex 2 (TSC2) gene expression analysis were performed by real-time PCR in heart muscle. Moreover, we assessed cardiac protein expression of ERK1/2, PI3K/AKT/mTOR, PTEN and TSC2.

Results

Cardiac phosphoser473-AKT and phosphoSer2448-mTOR were, respectively, increased by 46 and 38 % in the SE group when compared with SC group. miRNAs-21, 144, and 145 were, respectively, up-regulated in the SE group (152 %, 128, and 101 % relative increases), but miRNA-124 was decreased by 38 %. In SE group, PIK3α (targeted by miRNA-124) gene expression increased by 213 %, and Pten (targeted by miRNAs-21 and 144), and TSC2 (targeted by miRNA-145) were, respectively, decreased by 51 and 55 %. In addition, the swimming exercise increased protein levels of PIK3α (36 %) and phosphoThr1462-TSC2 (48 %), while it decreased PTEN (37 %) and TSC2 (22 %), which induced activation of PI3K/AKT/mTOR signaling pathway.

Conclusion

These findings are consistent with a model in which exercise may induce left ventricular hypertrophy, at least in part, changing the expression of specific miRNAs targeting the PIK3/AKT/mTOR signaling pathway and its negative regulators.

Keywords

SwimmingHeartHypertrophymicroRNAPI3KERK

Abbreviations

ANP

Atrial natriuretic polypeptide

LVH

Left ventricular hypertrophy

mTOR

Mammalian target of rapamycin

miRNA

microRNAs

PIK3α

Phosphoinositide-3-kinase catalytic alpha polypeptide

PIP2

Phosphatidylinositol-4,5-bisphosphate

PIP3

Phosphatidylinositol-3,4,5-trisphosphate

PTEN

Phosphatase and tensin homolog

PKC

Protein kinase C

TSC2

Tuberous sclerosis complex 2

α-Actin

Skeletal muscle α-actin

α-MHC

α-Myosin heavy chain

β-MHC

β-Myosin heavy chain

Copyright information

© Springer-Verlag Berlin Heidelberg 2013