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Effects of whole-body heat acclimation on cell injury and cytokine responses in peripheral blood mononuclear cells

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Abstract

To test the hypothesis that whole-body heat acclimation (HA) would increase peripheral blood mononuclear cells’ (PBMC) tolerance to heat shock (HS) and/or alter the release of cytokines (IL-1β, IL-6, IL-10 and TNF-α) to bacterial lipopolysaccharide (LPS), we heat acclimated nine subjects by exercising them for 100 min in a hot environment for 10 days. The subjects’ PBMC were separated and cultured on days 1 and 10 of HA pre- and post-exercise. Pre-exercise PBMC were allocated to three treatments: control (PRE, 37°C), HS (42.5°C for 2 h), or LPS (1 ng mL−1 for 24 h). Post-exercise samples were incubated at 37°C. PBMC lactate dehydrogenase release increased (p < 0.05) after HS but it was not different (p > 0.05) between days 1 and 10 (0.100 ± 0.012 and 0.102 ± 0.16 abs., respectively). LPS treatment induced an increased (p < 0.05) release of cytokines but HA did not alter this response (p > 0.05). Pre-exercise intracellular heat shock protein 72 (Hsp72) was higher (p < 0.05) on day 10 compared to day 1 of HA (13 ± 5 and 8 ± 5 ng mL−1, respectively). HS treatment caused a greater increase (p < 0.05) in Hsp72 than the exercise sessions on HA days 1 and 10. In addition, after HA, the Hsp72 response to HS was reduced (day 1, 129 ± 46; day 10, 80 ± 32 ng mL−1, p < 0.05). In conclusion, HA increases PBMC Hsp72 but it does not reduce cellular damage to HS or alter cytokine response to LPS. We speculate that the stress applied during HA is not sufficient to modify the PBMC response.

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Acknowledgments

We thank the volunteers for their participation. We acknowledge technical assistance of Lou Stevenson, Carol Morris, Walker Wharton, Karol Dokladny and Rebecca Lobb. The first author gratefully acknowledges the financial support by the Brazilian Government under CAPES-scholarship. This study was partially funded by National Institute of Arthritis and Musculoskeletal and Skin Diseases Grant AR40771 and by the Gatorade Student Award to Fabiano Amorim. The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Exercise Physiology Laboratory, Department of Health Exercise and Sports Sciences, University of New Mexico, MSC 04 2610, Albuquerque, NM, 87131-0001, USA

    Fabiano Amorim, Paulette Yamada, Robert Robergs & Suzanne Schneider

  2. Department of Internal Medicine, University of New Mexico, Albuquerque, NM, 87131, USA

    Pope Moseley

Authors
  1. Fabiano Amorim
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  2. Paulette Yamada
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  3. Robert Robergs
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  4. Suzanne Schneider
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  5. Pope Moseley
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Correspondence to Fabiano Amorim.

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Communicated by Susan Ward.

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Amorim, F., Yamada, P., Robergs, R. et al. Effects of whole-body heat acclimation on cell injury and cytokine responses in peripheral blood mononuclear cells. Eur J Appl Physiol 111, 1609–1618 (2011). https://doi.org/10.1007/s00421-010-1780-4

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  • DOI: https://doi.org/10.1007/s00421-010-1780-4

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