International Archives of Occupational and Environmental Health

, Volume 83, Issue 2, pp 217–223

Nasal blown secretion evaluation in specific occupational nasal challenges

Authors

    • Allergy and Immunology Unit and ISPESL Occupational Immunology and Allergy Laboratory, Fondazione ‘Salvatore Maugeri’Institute of Research and Care, Scientific Institute of Pavia
  • Gianni Pala
    • Allergy and Immunology Unit and ISPESL Occupational Immunology and Allergy Laboratory, Fondazione ‘Salvatore Maugeri’Institute of Research and Care, Scientific Institute of Pavia
  • Marta Pisati
    • Allergy and Immunology Unit and ISPESL Occupational Immunology and Allergy Laboratory, Fondazione ‘Salvatore Maugeri’Institute of Research and Care, Scientific Institute of Pavia
  • Luca Perfetti
    • Allergy and Immunology Unit and ISPESL Occupational Immunology and Allergy Laboratory, Fondazione ‘Salvatore Maugeri’Institute of Research and Care, Scientific Institute of Pavia
  • Gabriella Banchieri
    • Allergy and Immunology Unit and ISPESL Occupational Immunology and Allergy Laboratory, Fondazione ‘Salvatore Maugeri’Institute of Research and Care, Scientific Institute of Pavia
  • Gianna Moscato
    • Allergy and Immunology Unit and ISPESL Occupational Immunology and Allergy Laboratory, Fondazione ‘Salvatore Maugeri’Institute of Research and Care, Scientific Institute of Pavia
Original Article

DOI: 10.1007/s00420-009-0459-9

Cite this article as:
Pignatti, P., Pala, G., Pisati, M. et al. Int Arch Occup Environ Health (2010) 83: 217. doi:10.1007/s00420-009-0459-9

Abstract

Purpose

To investigate the usefulness of nasal blown secretion evaluation during specific nasal provocation test (sNPT) in diagnosing occupational rhinitis (OR).

Methods

To validate the method, nasal blown secretions from 103 healthy subjects and 30 allergic rhinitis patients were analyzed. Furthermore, we analyzed nasal blown secretions, collected before and after sNPT, of 29 subjects with work-related rhinitis symptoms (WRRS) who underwent the diagnostic pathway for OR. Rhinoscopy and nasal symptom score were used to define a positive sNPT.

Results

A total of 89.6% WRRS subjects provided suitable nasal secretions. Eosinophils significantly increased after positive sNPTs compared to negative ones (P = 0.006). Four percent and/or 1 × 104 eosinophils/ml was the cut-off for a significant post-sNPT eosinophil increase. A total of 4/13 (33%) WRRS subjects with negative sNPT, assessed by rhinoscopy and nasal symptom score, presented a significant post-sNPT nasal eosinophil increase, and were identified as possible OR.

Conclusion

Eosinophil evaluation in nasal blown secretions is an important tool in monitoring the response to occupational specific nasal challenges.

Keywords

Eosinophils Nasal provocation test Nasal blown secretions Occupational rhinitis Reference values

Abbreviations

sNPT

Specific nasal provocation test/challenge

OR

Occupational rhinitis

WRRS

Work-related rhinitis symptoms

AR

Allergic rhinitis

SD

Standard deviation

DTT

Dithithreitol

HMW

High molecular weight

LMW

Low molecular weight

Copyright information

© Springer-Verlag 2009