Histochemistry and Cell Biology

, Volume 110, Issue 5, pp 535–540

Localisation of gelatinase activity in epidermal hoof lamellae by in situ zymography

Authors

  • B. A. Mungall
    • Department of Companion Animal Sciences, School of Veterinary Science, The University of Queensland, Brisbane, Queensland 4072 Australia Tel. +61 7 3365 2350; Fax +61 7 3365 1899
  • Christopher C. Pollitt
    • Department of Companion Animal Sciences, School of Veterinary Science, The University of Queensland, Brisbane, Queensland 4072 Australia Tel. +61 7 3365 2350; Fax +61 7 3365 1899
  • Rita Collins
    • Vision, Touch and Hearing Research Centre, Department of Physiology and Pharmacology, The University of Queensland, Brisbane, Queensland 4072 Australia
ORIGINAL PAPER

DOI: 10.1007/s004180050315

Cite this article as:
Mungall, B., Pollitt, C. & Collins, R. Histochemistry (1998) 110: 535. doi:10.1007/s004180050315

Abstract

 In situ gelatin zymography is a technique, which utilises a gelatin-based emulsion overlay to detect and, more importantly, localise the gelatinase activity in underlying tissue. Gelatinase A [matrix metalloproteinase-2 (MMP-2)] and gelatinase B [matrix metalloproteinase-9 (MMP-9)] are present in equine hoof homogenates and supernatants from cultured hoof explants by SDS-PAGE gelatin zymography, and it has been assumed that the enzymes are derived solely from matrix and epithelia and not from other sources such as leucocytes. Using in situ zymography, gelatinases are shown to be localised within the equine epidermal hoof lamellae and, more specifically, are apparently produced by epidermal basal and/or parabasal cells. The pattern of expression correlates with that expected based on the progression of pathological changes observed during the onset of laminitis, thus providing further evidence that laminitis pathology probably arises as a result of inadequate local MMP regulation.

Copyright information

© Springer-Verlag Berlin Heidelberg 1998