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Functional characterisation of the YIPF protein family in mammalian cells

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Abstract

In this study, we carry out a systematic characterisation of the YIPF family of proteins with respect to their subcellular localisation profile, membrane topology and functional effects on the endomembrane system. YIPF proteins primarily localise to the Golgi complex and can be grouped into trans-Golgi-localising YIPFs (YIPF1 and YIPF2) and cis-Golgi-localising YIPFs (YIPF3, YIPF4 and YIPF5), with YIPF6 and YIPF7 showing a broader profile being distributed throughout the Golgi stack. YIPF proteins have a long soluble N-terminal region, which is orientated towards the cytosol, followed by 5 closely stacked transmembrane domains, and a C terminus, orientated towards the lumen of the Golgi. The significance of YIPF proteins for the maintenance of the morphology of the Golgi was tested by RNA interference, revealing a number of specific morphological changes to this organelle on their depletion. We propose a role for this family of proteins in regulating membrane dynamics in the endomembrane system.

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Acknowledgements

This work was partly funded by The Irish Research Council (IRC), the UCD Bioinformatics and Systems Biology PhD programme, and a Principal Investigator (PI) Grant (09/IN.1/B2604) from Science Foundation Ireland (SFI) to JCS. This work was carried out in the UCD Cell Screening Laboratory, supported by a grant from the UCD College of Science. The authors thank Maeve Long, George Galea and Mariana G. Bexiga for valuable feedback and discussion.

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  1. Tilen Kranjc

    Present address: Department of Clinical Biochemistry, Faculty of Pharmacy, University of Ljubljana, Aškerčeva 7, 1000, Ljubljana, Slovenia

Authors and Affiliations

  1. School of Biology and Environmental Science, University College Dublin, Dublin 4, Ireland

    Tilen Kranjc, Eugene Dempsey & Jeremy C. Simpson

  2. UCD Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Dublin 4, Ireland

    Tilen Kranjc, Eugene Dempsey, Gerard Cagney, Denis C. Shields & Jeremy C. Simpson

  3. School of Biomolecular and Biomedical Science, University College Dublin, Dublin 4, Ireland

    Gerard Cagney

  4. Department of Molecular Biosciences, Faculty of Life Sciences, Kyoto Sangyo University, Motoyama, Kamigamo, Kita, Kyoto, 603-8555, Japan

    Nobuhiro Nakamura

  5. School of Medicine and Medical Science, University College Dublin, Dublin 4, Ireland

    Denis C. Shields

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  1. Tilen Kranjc
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Correspondence to Jeremy C. Simpson.

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Kranjc, T., Dempsey, E., Cagney, G. et al. Functional characterisation of the YIPF protein family in mammalian cells. Histochem Cell Biol 147, 439–451 (2017). https://doi.org/10.1007/s00418-016-1527-3

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